{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:26:18Z","timestamp":1790753178596,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["FRM FDT201904007918"],"award-info":[{"award-number":["FRM FDT201904007918"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["ANR-17-CE14-0032"],"award-info":[{"award-number":["ANR-17-CE14-0032"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["FRM FDT201904007918"],"award-info":[{"award-number":["FRM FDT201904007918"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-17-CE14-0032"],"award-info":[{"award-number":["ANR-17-CE14-0032"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fili\u00e8res Maladies Rares OSCAR and ORKID","award":["FRM FDT201904007918"],"award-info":[{"award-number":["FRM FDT201904007918"]}]},{"name":"Fili\u00e8res Maladies Rares OSCAR and ORKID","award":["ANR-17-CE14-0032"],"award-info":[{"award-number":["ANR-17-CE14-0032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Many genomic, anatomical and functional differences exist between the medullary (MTAL) and the cortical thick ascending limb of the loop of Henle (CTAL), including a higher expression of claudin-10 (CLDN10) in the MTAL than in the CTAL. Therefore, we assessed to what extent the Cldn10 gene expression is a determinant of differential gene expression between MTAL and CTAL. RNAs extracted from CTAL and MTAL microdissected from wild type (WT) and Cldn10 knock out mice (cKO) were analyzed by RNAseq. Differential and enrichment analyses (GSEA) were performed with interactive R Shiny software. Between WT and cKO MTAL, 637 genes were differentially expressed, whereas only 76 were differentially expressed between WT and cKO CTAL. Gene expression patterns and GSEA analyses in all replicates showed that WT MTAL did not cluster with the other replicates; no hierarchical clustering could be found between WT CTAL, cKO CTAL and cKO MTAL. Compared to WT replicates, cKO replicates were enriched in Cldn16, Cldn19, Pth1r, (parathyroid hormone receptor type 1), Casr (calcium sensing receptor) and Vdr (Vitamin D Receptor) mRNA in both the cortex and medulla. Cldn10 is associated with gene expression patterns, including genes specifically involved in divalent cations reabsorption in the TAL.<\/jats:p>","DOI":"10.3390\/ijms25074008","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T11:01:41Z","timestamp":1712142101000},"page":"4008","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0282-8640","authenticated-orcid":false,"given":"Gaelle","family":"Brideau","sequence":"first","affiliation":[{"name":"Centre de Recherche des Cordeliers, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"},{"name":"Centre National de la Recherche Scientifique, Equipe Mixte de Recherche 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8228-Laboratoire de Physiologie R\u00e9nale et Tubulopathies, F-75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7065-9124","authenticated-orcid":false,"given":"Gilles","family":"Crambert","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"},{"name":"Centre National de la Recherche Scientifique, Equipe Mixte de Recherche 8228-Laboratoire de Physiologie R\u00e9nale et Tubulopathies, F-75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3478-1576","authenticated-orcid":false,"given":"Dominik","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Gastroenterology, Nephrology and Metabolic Diseases, Charit\u00e9-Universit\u00e4tsmedizin Berlin, DE-13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovana","family":"Bro\u0107i\u0107","sequence":"additional","affiliation":[{"name":"Paris Brain Institute (ICM), H\u00f4pital Piti\u00e9-Salp\u00eatri\u00e8re, Inserm U 1127, CNRS UMR 7225, Sorbonne Universit\u00e9, Data Analysis Core Platform, F-75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4140-180X","authenticated-orcid":false,"given":"Emeline","family":"Cherchame","sequence":"additional","affiliation":[{"name":"Paris Brain Institute (ICM), H\u00f4pital Piti\u00e9-Salp\u00eatri\u00e8re, Inserm U 1127, CNRS UMR 7225, Sorbonne Universit\u00e9, Data Analysis Core Platform, F-75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Houillier","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"},{"name":"Centre National de la Recherche Scientifique, Equipe Mixte de Recherche 8228-Laboratoire de Physiologie R\u00e9nale et Tubulopathies, F-75006 Paris, France"},{"name":"Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Europ\u00e9en Georges Pompidou, Service de Physiologie, F-75015 Paris, France"},{"name":"Centre de R\u00e9f\u00e9rence des Maladies R\u00e9nales H\u00e9r\u00e9ditaires de l\u2019Enfant et de l\u2019Adulte (MARHEA), The European Rare Kidney Disease Reference Network (ERKNet), F-75015 Paris, France"},{"name":"Centre de R\u00e9f\u00e9rence des Maladies Rares du Calcium et du Phosphate, The European Reference Network on Rare Endocrine Conditions (Endo-ERN), F-75015 Paris, France"},{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, 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