{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:27:47Z","timestamp":1792542467704,"version":"4.5.0"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,4,7]],"date-time":"2024-04-07T00:00:00Z","timestamp":1712448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China","award":["SKL-KF202322"],"award-info":[{"award-number":["SKL-KF202322"]}]},{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China","award":["SKL-KF202207"],"award-info":[{"award-number":["SKL-KF202207"]}]},{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China","award":["2021YFH0110"],"award-info":[{"award-number":["2021YFH0110"]}]},{"name":"International Cooperation Program of Science and Technology Department of Sichuan Province","award":["SKL-KF202322"],"award-info":[{"award-number":["SKL-KF202322"]}]},{"name":"International Cooperation Program of Science and Technology Department of Sichuan Province","award":["SKL-KF202207"],"award-info":[{"award-number":["SKL-KF202207"]}]},{"name":"International Cooperation Program of Science and Technology Department of Sichuan Province","award":["2021YFH0110"],"award-info":[{"award-number":["2021YFH0110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Leaf rolling is regarded as an important morphological trait in wheat breeding. Moderate leaf rolling is helpful to keep leaves upright and improve the photosynthesis of plants, leading to increased yield. However, studies on the identification of genomic regions\/genes associated with rolling leaf have been reported less frequently in wheat. In this study, a rolling leaf mutant, T73, which has paired spikelets, dwarfism, and delayed heading traits, was obtained from a common wheat landrace through ethyl methanesulfonate mutagenesis. The rlT73 mutation caused an increase in the number of epidermal cells on the abaxial side and the shrinkage of bulliform cells on the adaxial side, leading to an adaxially rolling leaf phenotype. Genetic analysis showed that the rolling leaf phenotype was controlled by a single recessive gene. Further Wheat55K single nucleotide polymorphism array-based bulked segregant analysis and molecular marker mapping delimited rlT73 to a physical interval of 300.29\u2013318.33 Mb on the chromosome arm 1BL in the Chinese Spring genome. We show that a point mutation at the miRNA165\/166 binding site of the HD zipper class III transcription factor on 1BL altered its transcriptional level, which may be responsible for the rolling leaf phenotype. Our results suggest the important role of rlT73 in regulating wheat leaf development and the potential of miRNA-based gene regulation for crop trait improvement.<\/jats:p>","DOI":"10.3390\/ijms25074103","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T10:11:55Z","timestamp":1712571115000},"page":"4103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterization and Mapping of a Rolling Leaf Mutant Allele rlT73 on Chromosome 1BL of Wheat"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4753-3683","authenticated-orcid":false,"given":"Lin","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijuan","family":"Gan","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhuo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Hu","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilin","family":"Du","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqin","family":"Li","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanghui","family":"Zeng","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Wu","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Hao","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6167-6723","authenticated-orcid":false,"given":"Shunzong","family":"Ning","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongwei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9005-8705","authenticated-orcid":false,"given":"Lianquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bihua","family":"Wu","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengcai","family":"Liu","sequence":"additional","affiliation":[{"name":"Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1111\/tpj.13728","article-title":"CLD1\/SRL1 modulates leaf rolling by affecting cell wall formation, epidermis integrity and water homeostasis in rice","volume":"92","author":"Li","year":"2017","journal-title":"Plant J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1104\/pp.112.199968","article-title":"SEMI-ROLLED LEAF1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells","volume":"159","author":"Xiang","year":"2012","journal-title":"Plant Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"897623","DOI":"10.3389\/fpls.2022.897623","article-title":"A transcription factor TaMYB5 modulates leaf rolling in 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