{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T14:23:56Z","timestamp":1779373436111,"version":"3.53.1"},"reference-count":53,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,14]],"date-time":"2024-04-14T00:00:00Z","timestamp":1713052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["81973510"],"award-info":[{"award-number":["81973510"]}]},{"name":"National Natural Science Foundation of China","award":["KJ2021A1227"],"award-info":[{"award-number":["KJ2021A1227"]}]},{"name":"National Natural Science Foundation of China","award":["2023AH050842"],"award-info":[{"award-number":["2023AH050842"]}]},{"name":"National Natural Science Foundation of China","award":["gxbjZD04"],"award-info":[{"award-number":["gxbjZD04"]}]},{"name":"Key Natural Science Research Projects of Anhui Universities","award":["81973510"],"award-info":[{"award-number":["81973510"]}]},{"name":"Key Natural Science Research Projects of Anhui Universities","award":["KJ2021A1227"],"award-info":[{"award-number":["KJ2021A1227"]}]},{"name":"Key Natural Science Research Projects of Anhui Universities","award":["2023AH050842"],"award-info":[{"award-number":["2023AH050842"]}]},{"name":"Key Natural Science Research Projects of Anhui Universities","award":["gxbjZD04"],"award-info":[{"award-number":["gxbjZD04"]}]},{"name":"Key academic funding project for top talents in disciplines (majors) in Anhui Province\u2019s universities","award":["81973510"],"award-info":[{"award-number":["81973510"]}]},{"name":"Key academic funding project for top talents in disciplines (majors) in Anhui Province\u2019s universities","award":["KJ2021A1227"],"award-info":[{"award-number":["KJ2021A1227"]}]},{"name":"Key academic funding project for top talents in disciplines (majors) in Anhui Province\u2019s universities","award":["2023AH050842"],"award-info":[{"award-number":["2023AH050842"]}]},{"name":"Key academic funding project for top talents in disciplines (majors) in Anhui Province\u2019s universities","award":["gxbjZD04"],"award-info":[{"award-number":["gxbjZD04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The protective effects of hydrogen sulfide (H2S) against ischemic brain injury and its role in promoting angiogenesis have been established. However, the specific mechanism underlying these effects remains unclear. This study is designed to investigate the regulatory impact and mechanism of H2S on VEGFR2 phosphorylation. Following expression and purification, the recombinant His-VEGFR2 protein was subjected to LC-PRM\/MS analysis to identify the phosphorylation sites of VEGFR2 upon NaHS treatment. Adenovirus infection was used to transfect primary rat brain artery endothelial cells (BAECs) with the Ad-VEGFR2WT, Ad-VEGFR2Y797F, and Ad-VEGFR2S799A plasmids. The expression of VEGFR2 and recombinant Flag-VEGFR2, along with Akt phosphorylation, cell proliferation, and LDH levels, was assessed. The migratory capacity and tube-forming potential of BAECs were assessed using wound healing, transwell, and tube formation assays. NaHS notably enhanced the phosphorylation of VEGFR2 at Tyr797 and Ser799 sites. These phosphorylation sites were identified as crucial for mediating the protective effects of NaHS against hypoxia\u2013reoxygenation (H\/R) injury. NaHS significantly enhanced the Akt phosphorylation, migratory capacity, and tube formation of BAECs and upregulated the expression of VEGFR2 and recombinant proteins. These findings suggest that Tyr797 and Ser799 sites of VEGFR2 serve as crucial mediators of H2S-induced pro-angiogenic effects and protection against H\/R injury.<\/jats:p>","DOI":"10.3390\/ijms25084340","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T10:24:26Z","timestamp":1713176666000},"page":"4340","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hydrogen Sulfide Exerted a Pro-Angiogenic Role by Promoting the Phosphorylation of VEGFR2 at Tyr797 and Ser799 Sites in Hypoxia\u2013Reoxygenation Injury"],"prefix":"10.3390","volume":"25","author":[{"given":"Sen","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfeng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Clinical Medical College, Anhui Medical University, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yining","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyue","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1177\/17474930211065917","article-title":"World Stroke Organization (WSO): Global Stroke Fact Sheet 2022","volume":"17","author":"Feigin","year":"2022","journal-title":"Int. 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