{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:49:58Z","timestamp":1790621398487,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI23\/00260"],"award-info":[{"award-number":["PI23\/00260"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI17\/01397"],"award-info":[{"award-number":["PI17\/01397"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CIBERCV"],"award-info":[{"award-number":["CIBERCV"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Spanish Society of Cardiology (Proyectos de la FEC para Investigaci\u00f3n B\u00e1sica en Cardiolog\u00eda 2018, Sociedad Espa\u00f1ola de Cardiolog\u00eda)","award":["PI23\/00260"],"award-info":[{"award-number":["PI23\/00260"]}]},{"name":"Spanish Society of Cardiology (Proyectos de la FEC para Investigaci\u00f3n B\u00e1sica en Cardiolog\u00eda 2018, Sociedad Espa\u00f1ola de Cardiolog\u00eda)","award":["PI17\/01397"],"award-info":[{"award-number":["PI17\/01397"]}]},{"name":"Spanish Society of Cardiology (Proyectos de la FEC para Investigaci\u00f3n B\u00e1sica en Cardiolog\u00eda 2018, Sociedad Espa\u00f1ola de Cardiolog\u00eda)","award":["CIBERCV"],"award-info":[{"award-number":["CIBERCV"]}]},{"name":"European Regional Development Fund","award":["PI23\/00260"],"award-info":[{"award-number":["PI23\/00260"]}]},{"name":"European Regional Development Fund","award":["PI17\/01397"],"award-info":[{"award-number":["PI17\/01397"]}]},{"name":"European Regional Development Fund","award":["CIBERCV"],"award-info":[{"award-number":["CIBERCV"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Succinate dehydrogenase inhibition with malonate during initial reperfusion reduces myocardial infarct size in both isolated mouse hearts subjected to global ischemia and in in situ pig hearts subjected to transient coronary ligature. However, the long-term effects of acute malonate treatment are unknown. Here, we investigated whether the protective effects of succinate dehydrogenase inhibition extend to a reduction in scar size and adverse left ventricular remodeling 28 days after myocardial infarction. Initially, ten wild-type mice were subjected to 45 min of left anterior descending coronary artery (LAD) occlusion, followed by 24 h of reperfusion, and were infused during the first 15 min of reperfusion with saline with or without disodium malonate (10 mg\/kg\/min, 120 \u03bcL\/kg\/min). Malonate-treated mice depicted a significant reduction in infarct size (15.47 \u00b1 3.40% of area at risk vs. 29.34 \u00b1 4.44% in control animals, p &lt; 0.05), assessed using triphenyltetrazolium chloride. Additional animals were then subjected to a 45 min LAD ligature, followed by 28 days of reperfusion. Treatment with a single dose of malonate during the first 15 min of reperfusion induced a significant reduction in scar area, measured using Picrosirius Red staining (11.94 \u00b1 1.70% of left ventricular area (n = 5) vs. 23.25 \u00b1 2.67% (n = 9), p &lt; 0.05), an effect associated with improved ejection fraction 28 days after infarction, as determined using echocardiography, and an attenuated enhancement in expression of the pro-inflammatory and fibrotic markers NF-\u03baB and Smad2\/3 in remote myocardium. In conclusion, a reversible inhibition of succinate dehydrogenase with a single dose of malonate at the onset of reperfusion has long-term protective effects in mice subjected to transient coronary occlusion.<\/jats:p>","DOI":"10.3390\/ijms25084366","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T11:53:53Z","timestamp":1713182033000},"page":"4366","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Long-Term Protective Effects of Succinate Dehydrogenase Inhibition during Reperfusion with Malonate on Post-Infarction Left Ventricular Scar and Remodeling in Mice"],"prefix":"10.3390","volume":"25","author":[{"given":"Laura","family":"Valls-Lacalle","sequence":"first","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9201-9117","authenticated-orcid":false,"given":"Marta","family":"Consegal","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freddy G.","family":"Ganse","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Y\u00e1\u00f1ez-Bisbe","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Pastor","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisol","family":"Ruiz-Meana","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4736-5010","authenticated-orcid":false,"given":"Javier","family":"Inserte","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bego\u00f1a","family":"Benito","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Cardiology Department, Hospital Universitari Vall d\u2019Hebron, 08035 Barcelona, Spain"},{"name":"Department of Medicine, Universitat Aut\u00f2noma de Barcelona, 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Ferreira-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Cardiology Department, Hospital Universitari Vall d\u2019Hebron, 08035 Barcelona, Spain"},{"name":"Department of Medicine, Universitat Aut\u00f2noma de Barcelona, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2930-8773","authenticated-orcid":false,"given":"Antonio","family":"Rodr\u00edguez-Sinovas","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Vall d\u2019Hebron University Hospital and Research Institute, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, 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