{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:33:02Z","timestamp":1790371982846,"version":"4.1.0"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Temas Libres","award":["930\/17"],"award-info":[{"award-number":["930\/17"]}]},{"name":"Temas Libres","award":["1181699"],"award-info":[{"award-number":["1181699"]}]},{"name":"Temas Libres","award":["1231596"],"award-info":[{"award-number":["1231596"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Metabolic dysfunction-associated steatotic liver disease (MASLD) is a complex disorder whose prevalence is rapidly growing in South America. The disturbances in the microbiota\u2013gut\u2013liver axis impact the liver damaging processes toward fibrosis. Gut microbiota status is shaped by dietary and lifestyle factors, depending on geographic location. We aimed to identify microbial signatures in a group of Chilean MASLD patients. Forty subjects were recruited, including healthy controls (HCs), overweight\/obese subjects (Ow\/Ob), patients with MASLD without fibrosis (MASLD\/F\u2212), and MASLD with fibrosis (MASLD\/F+). Both MASLD and fibrosis were detected through elastography and\/or biopsy, and fecal microbiota were analyzed through deep sequencing. Despite no differences in \u03b1- and \u03b2-diversity among all groups, a higher abundance of Bilophila and a lower presence of Defluviitaleaceae, Lachnospiraceae ND3007, and Coprobacter was found in MASLD\/F\u2212 and MASLD\/F+, compared to HC. Ruminococcaceae UCG-013 and Sellimonas were more abundant in MASLD\/F+ than in Ow\/Ob; both significantly differed between MASLD\/F\u2212 and MASLD\/F+, compared to HC. Significant positive correlations were observed between liver stiffness and Bifidobacterium, Prevotella, Sarcina, and Acidaminococcus abundance. Our results show that MASLD is associated with changes in bacterial taxa that are known to be involved in bile acid metabolism and SCFA production, with some of them being more specifically linked to fibrosis.<\/jats:p>","DOI":"10.3390\/ijms25084387","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T09:16:33Z","timestamp":1713258993000},"page":"4387","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Alteration of Gut Microbiota Composition in the Progression of Liver Damage in Patients with Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)"],"prefix":"10.3390","volume":"25","author":[{"given":"Alejandra","family":"Zazueta","sequence":"first","affiliation":[{"name":"Microbiology and Mycology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5145-303X","authenticated-orcid":false,"given":"Luc\u00eda","family":"Valenzuela-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-8593","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"Ortiz-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araceli","family":"Pinto-Le\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Torres","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danette","family":"Gui\u00f1ez","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Aliaga","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Merino","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Covarrubias","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"P\u00e9rez de Arce","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1ximo","family":"Cattaneo","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro","family":"Urz\u00faa","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Roblero","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Poniachik","sequence":"additional","affiliation":[{"name":"Unit of Gastroenterology, Department of Medicine, Hospital Clinico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8833-6558","authenticated-orcid":false,"given":"Mart\u00edn","family":"Gotteland","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Faculty of Medicine, University of Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Magne","sequence":"additional","affiliation":[{"name":"Microbiology and Mycology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroll Jenny","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratory of Immuno-Gastroenterology, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.ecl.2023.01.001","article-title":"Pathobiology of Metabolic-Associated Fatty Liver Disease","volume":"52","author":"Fouda","year":"2023","journal-title":"Endocrinol. 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