{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T18:10:24Z","timestamp":1772647824050,"version":"3.50.1"},"reference-count":87,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T00:00:00Z","timestamp":1714348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universit\u00e0 del Piemonte Orientale","award":["DSF-FAR 2016"],"award-info":[{"award-number":["DSF-FAR 2016"]}]},{"name":"Universit\u00e0 del Piemonte Orientale","award":["DM 1062\/2021"],"award-info":[{"award-number":["DM 1062\/2021"]}]},{"name":"European Union\u2014Fondo Sociale Europeo Recovery Assistance for Cohesion","award":["DSF-FAR 2016"],"award-info":[{"award-number":["DSF-FAR 2016"]}]},{"name":"European Union\u2014Fondo Sociale Europeo Recovery Assistance for Cohesion","award":["DM 1062\/2021"],"award-info":[{"award-number":["DM 1062\/2021"]}]},{"name":"Territories of the European Union (FSE-REACT-EU), Programma Operativo Nazionale (PON), Azione IV.4","award":["DSF-FAR 2016"],"award-info":[{"award-number":["DSF-FAR 2016"]}]},{"name":"Territories of the European Union (FSE-REACT-EU), Programma Operativo Nazionale (PON), Azione IV.4","award":["DM 1062\/2021"],"award-info":[{"award-number":["DM 1062\/2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Implant therapy is a common treatment option in dentistry and orthopedics, but its application is often associated with an increased risk of microbial contamination of the implant surfaces that cause bone tissue impairment. This study aims to develop two silver-enriched platelet-rich plasma (PRP) multifunctional scaffolds active at the same time in preventing implant-associated infections and stimulating bone regeneration. Commercial silver lactate (L) and newly synthesized silver deoxycholate:\u03b2-Cyclodextrin (B), were studied in vitro. Initially, the antimicrobial activity of the two silver soluble forms and the PRP enriched with the two silver forms has been studied on microbial planktonic cells. At the same time, the biocompatibility of silver-enriched PRPs has been assessed by an MTT test on human primary osteoblasts (hOBs). Afterwards, an investigation was conducted to evaluate the activity of selected concentrations and forms of silver-enriched PRPs in inhibiting microbial biofilm formation and stimulating hOB differentiation. PRP-L (0.3 \u00b5g\/mm2) and PRP-B (0.2 \u00b5g\/mm2) counteract Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans planktonic cell growth and biofilm formation, preserving hOB viability without interfering with their differentiation capability. Overall, the results obtained suggest that L- and B-enriched PRPs represent a promising preventive strategy against biofilm-related implant infections and demonstrate a new silver formulation that, together with increasing fibrin binding protecting silver in truncated cone-shaped cyclic oligosaccharides, achieved comparable inhibitory results on prokaryotic cells at a lower concentration.<\/jats:p>","DOI":"10.3390\/ijms25094842","type":"journal-article","created":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T03:30:49Z","timestamp":1714534249000},"page":"4842","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An In Vitro Study on the Application of Silver-Doped Platelet-Rich Plasma in the Prevention of Post-Implant-Associated Infections"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9599-544X","authenticated-orcid":false,"given":"Chiara","family":"Ceresa","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale \u201cA. Avogadro\u201d, 28100 Novara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3003-989X","authenticated-orcid":false,"given":"Fabio","family":"Travagin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale \u201cA. Avogadro\u201d, 28100 Novara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8673-5769","authenticated-orcid":false,"given":"Alice","family":"Marchetti","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale \u201cA. Avogadro\u201d, 28100 Novara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4022-5602","authenticated-orcid":false,"given":"Francesco","family":"Tessarolo","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering & BIOtech, University of Trento, 38123 Trento, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3505-1919","authenticated-orcid":false,"given":"Letizia","family":"Fracchia","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale \u201cA. Avogadro\u201d, 28100 Novara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7117-0347","authenticated-orcid":false,"given":"Giovanni Battista","family":"Giovenzana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale \u201cA. Avogadro\u201d, 28100 Novara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-8702","authenticated-orcid":false,"given":"Michela","family":"Bosetti","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale \u201cA. Avogadro\u201d, 28100 Novara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Szcz\u0119sny, G., Kopec, M., Politis, D.J., Kowalewski, Z.L., \u0141azarski, A., and Szolc, T. (2022). A Review on Biomaterials for Orthopaedic Surgery and Traumatology: From Past to Present. Materials, 15.","DOI":"10.3390\/ma15103622"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mastrangelo, F. (2023). New Implant Materials. Materials, 16.","DOI":"10.3390\/ma16134525"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Al-Shalawi, F.D., Mohamed Ariff, A.H., Jung, D.W., Mohd Ariffin, M.K.A., Seng Kim, C.L., Brabazon, D., and Al-Osaimi, M.O. (2023). Biomaterials as Implants in the Orthopedic Field for Regenerative Medicine: Metal versus Synthetic Polymers. Polymers, 15.","DOI":"10.3390\/polym15122601"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Korsch, M., Marten, S.M., Stoll, D., Prechtl, C., and D\u00f6tsch, A. (2021). Microbiological findings in early and late implant loss: An observational clinical case-controlled study. BMC Oral Health, 21.","DOI":"10.1186\/s12903-021-01439-w"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S106","DOI":"10.1016\/j.injury.2020.11.050","article-title":"The rationale behind implant coatings to promote osteointegration, bone healing or regeneration","volume":"52","author":"Borcherding","year":"2021","journal-title":"Injury"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Silva, R.C.S., Agrelli, A., Andrade, A.N., Mendes-Marques, C.L., Arruda, I.R.S., Santos, L.R.L., Vasconcelos, N.F., and Machado, G. (2022). Titanium Dental Implants: An Overview of Applied Nanobiotechnology to Improve Biocompatibility and Prevent Infections. Materials, 15.","DOI":"10.3390\/ma15093150"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e01067","DOI":"10.1016\/j.heliyon.2018.e01067","article-title":"Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention","volume":"4","author":"Khatoon","year":"2018","journal-title":"Heliyon"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.3389\/fimmu.2019.01724","article-title":"Chronic Implant-Related Bone Infections-Can Immune Modulation be a Therapeutic Strategy?","volume":"10","author":"Seebach","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Merch\u00e1n, E.C., Davidson, D.J., and Liddle, A.D. (2021). Recent Strategies to Combat Infections from Biofilm-Forming Bacteria on Orthopaedic Implants. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910243"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Oliva, A., Miele, M.C., Al Ismail, D., Di Timoteo, F., De Angelis, M., Rosa, L., Cutone, A., Venditti, M., Mascellino, M.T., and Valenti, P. (2021). Challenges in the Microbiological Diagnosis of Implant-Associated Infections: A Summary of the Current Knowledge. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.750460"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Chen, X., Zhou, J., Qian, Y., and Zhao, L. (2023). Antibacterial coatings on orthopedic implants. Mater. Today Bio, 19.","DOI":"10.1016\/j.mtbio.2023.100586"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.2147\/TCRM.S139795","article-title":"Oral microbiome and peri-implant diseases: Where are we now?","volume":"13","author":"Pokrowiecki","year":"2017","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.cjtee.2020.05.009","article-title":"New perspectives on traumatic bone infections","volume":"23","author":"Tang","year":"2020","journal-title":"Chin. J. Traumatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"820843","DOI":"10.3389\/fimmu.2022.820843","article-title":"Dental and Orthopaedic Implant Loosening: Overlap in Gene Expression Regulation","volume":"13","author":"Schluessel","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.jhin.2020.04.026","article-title":"Pathogenesis of Staphylococcus epidermidis in prosthetic joint infections: Can identification of virulence genes differentiate between infecting and commensal strains?","volume":"105","author":"Benito","year":"2020","journal-title":"J. Hosp. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s40729-021-00338-7","article-title":"A systematic review on the implication of Candida in peri-implantitis","volume":"7","year":"2021","journal-title":"Int. J. Implant. Dent."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Lu, Y., Cai, W.J., Ren, Z., and Han, P. (2022). The Role of Staphylococcal Biofilm on the Surface of Implants in Orthopedic Infection. Microorganisms, 10.","DOI":"10.3390\/microorganisms10101909"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"103994","DOI":"10.1016\/j.isci.2022.103994","article-title":"Cross-kingdom microbial interactions in dental implant-related infections: Is Candida albicans a new villain?","volume":"25","author":"Souza","year":"2022","journal-title":"iScience"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.cmi.2019.08.006","article-title":"Pathogenesis and management of fracture-related infection","volume":"26","author":"Depypere","year":"2020","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00084-17","DOI":"10.1128\/CMR.00084-17","article-title":"Staphylococcal Osteomyelitis: Disease Progression, Treatment Challenges, and Future Directions","volume":"31","author":"Kavanagh","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.ajpath.2018.10.017","article-title":"Antibiotic Perturbation of Gut Microbiota Dysregulates Osteoimmune Cross Talk in Postpubertal Skeletal Development","volume":"189","author":"Steinkamp","year":"2019","journal-title":"Am. J. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1097\/BOT.0000000000001615","article-title":"Fracture-Related Infection (FRI) consensus group. Evidence-Based Recommendations for Local Antimicrobial Strategies and Dead Space Management in Fracture-Related Infection","volume":"34","author":"Metsemakers","year":"2020","journal-title":"J. Orthop. Trauma"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"80244","DOI":"10.1039\/C6RA09583F","article-title":"Biofilm formation in total hip arthroplasty: Prevention and treatment","volume":"6","author":"Davidson","year":"2016","journal-title":"Rsc Adv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e2000310","DOI":"10.1002\/adhm.202000310","article-title":"Biomaterials against Bone Infection","volume":"9","author":"Lozano","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1089\/sur.2008.9941","article-title":"History of the medical use of silver","volume":"10","author":"Alexander","year":"2009","journal-title":"Surg. Infect."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1089\/sur.2011.097","article-title":"Use of silver in the prevention and treatment of infections: Silver review","volume":"14","author":"Politano","year":"2013","journal-title":"Surg. Infect."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/S0142-9612(01)00198-3","article-title":"Silver coated materials for external fixation devices: In vitro biocompatibility and genotoxicity","volume":"23","author":"Bosetti","year":"2002","journal-title":"Biomaterials"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1517\/13543776.15.2.125","article-title":"Silver and its application as an antimicrobial agent","volume":"15","author":"Melaiye","year":"2005","journal-title":"Expert Opin. Ther. Pat."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/j.biotechadv.2018.05.004","article-title":"Silver bullets: A new lustre on an old antimicrobial agent","volume":"36","author":"Sim","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1159\/000093928","article-title":"Silver in health care: Antimicrobial effects and safety in use","volume":"33","author":"Lansdown","year":"2006","journal-title":"Curr. Probl. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Everts, P., Onishi, K., Jayaram, P., Lana, J.F., and Mautner, K. (2020). Platelet-Rich Plasma: New Performance Understandings and Therapeutic Considerations in 2020. Int. J. Mol. Sci., 21.","DOI":"10.20944\/preprints202010.0069.v1"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1517\/14712598.2016.1118458","article-title":"Platelet-rich plasma for bone healing and regeneration","volume":"16","author":"Oryan","year":"2016","journal-title":"Expert Opin. Biol. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"16036","DOI":"10.1038\/boneres.2016.36","article-title":"Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering","volume":"4","author":"Fernandes","year":"2016","journal-title":"Bone Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"153","DOI":"10.15171\/joddd.2018.024","article-title":"Comparative evaluation of platelet rich plasma in socket healing and bone regeneration after surgical removal of impacted mandibular third molars","volume":"12","author":"Bhujbal","year":"2018","journal-title":"J. Dent. Res. Dent. Clin. Dent. Prospect"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/adj.12754","article-title":"Platelet-rich plasma and regenerative dentistry","volume":"65","author":"Xu","year":"2020","journal-title":"Aust. Dent. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"151528","DOI":"10.1016\/j.aanat.2020.151528","article-title":"The use of plasma rich in growth factors (PRGF) in guided tissue regeneration and guided bone regeneration. A review of histological, immunohistochemical, histomorphometrical, radiological and clinical results in humans","volume":"231","author":"Solakoglu","year":"2020","journal-title":"Ann. Anat."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e1079","DOI":"10.3928\/01477447-20151120-04","article-title":"Platelet-Rich Plasma in the Animal Long-Bone Model: An Analysis of Basic Science Evidence","volume":"38","author":"Gianakos","year":"2015","journal-title":"Orthopedics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.joms.2011.03.028","article-title":"Comparative radiographic evaluation of alveolar bone healing associated with autologous platelet-rich plasma after impacted mandibular third molar surgery","volume":"70","year":"2012","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1002\/jor.24786","article-title":"The platelet-rich plasma and mesenchymal stem cell milieu: A review of therapeutic effects on bone healing","volume":"38","author":"Liebig","year":"2020","journal-title":"J. Orthop. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1111\/j.1600-0501.2005.01203.x","article-title":"The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts","volume":"17","author":"Graziani","year":"2006","journal-title":"Clin. Oral. Implant. Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kobayashi, E., Fujioka-Kobayashi, M., Sculean, A., Chappuis, V., Buser, D., Schaller, B., D\u0151ri, F., and Miron, R.J. (2017). Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour. BMC Oral Health, 17.","DOI":"10.1186\/s12903-017-0381-6"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.31616\/asj.2019.0048","article-title":"Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction","volume":"14","author":"Kinoshita","year":"2020","journal-title":"Asian Spine J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1089\/ten.tea.2009.0832","article-title":"The effect of platelet-rich plasma in vitro on primary cells: Rat osteoblast-like cells and human endothelial cells","volume":"16","author":"Mooren","year":"2010","journal-title":"Tissue Eng. Part A"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1054\/ijom.2003.0492","article-title":"Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study","volume":"33","author":"Arpornmaeklong","year":"2004","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"595","DOI":"10.4161\/19336918.2014.972785","article-title":"Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton","volume":"8","author":"Casati","year":"2014","journal-title":"Cell Adh. Migr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.1600-0765.2006.00892.x","article-title":"Platelet-rich plasma\/osteoblasts complex induces bone formation via osteoblastic differentiation following subcutaneous transplantation","volume":"41","author":"Goto","year":"2006","journal-title":"J. Periodontal. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1159\/000492415","article-title":"Platelet-Rich Plasma in Tissue Engineering: Hype and Hope","volume":"59","author":"Lang","year":"2018","journal-title":"Eur. Surg. Res."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Roffi, A., Filardo, G., Kon, E., and Marcacci, M. (2013). Does PRP enhance bone integration with grafts, graft substitutes, or implants? A systematic review. BMC Musculoskelet. Disord., 14.","DOI":"10.1186\/1471-2474-14-330"},{"key":"ref_49","first-page":"ZC26","article-title":"Effect of Platelet-Rich-Plasma (PRP) and Implant Surface Topography on Implant Stability and Bone","volume":"8","author":"Kundu","year":"2014","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1111\/clr.13532","article-title":"Effect of platelet-rich plasma on peri-implant trabecular bone volume and architecture: A preclinical micro-CT study in beagle dogs","volume":"30","author":"Huang","year":"2019","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Qu, C., Luo, F., Hong, G., and Wan, Q. (2021). Effects of platelet concentrates on implant stability and marginal bone loss: A systematic review and meta-analysis. BMC Oral Health, 21.","DOI":"10.1186\/s12903-021-01929-x"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1248\/cpb.c16-00779","article-title":"Potential Use of Cyclodextrins as Drug Carriers and Active Pharmaceutical Ingredients","volume":"65","author":"Arima","year":"2017","journal-title":"Chem. Pharm. Bull."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0378-5173(91)90229-H","article-title":"Studies on complexation between \u03b2-Ciclodextrin and bile salts","volume":"74","author":"Tan","year":"1991","journal-title":"Int. J. Pharm."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5489","DOI":"10.1021\/la9817359","article-title":"Complexation of sodium cholate and sodium deoxycholate by \u03b2-Cyclodextrin and derivatives","volume":"15","author":"Cabrer","year":"1999","journal-title":"Langmuir"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.msec.2016.10.001","article-title":"Fabrication and characterization of platelet-rich plasma scaffolds for tissue engineering applications","volume":"71","author":"Vojdani","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"257","DOI":"10.5301\/jva.5000142","article-title":"Hemodialysis catheter thrombi: Visualization and quantification of microstructures and cellular composition","volume":"14","author":"Lucas","year":"2013","journal-title":"J. Vasc. Access"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ceresa, C., Fracchia, L., Marchetti, A., Rinaldi, M., and Bosetti, M. (2019). Injectable Scaffolds Enriched with Silver to Inhibit Bacterial Invasion in Tissue Regeneration. Materials, 12.","DOI":"10.3390\/ma12121931"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1080\/09537104.2017.1293807","article-title":"Effects of an injectable platelet-rich fibrin on osteoblast behavior and bone tissue formation in comparison to platelet-rich plasma","volume":"29","author":"Wang","year":"2018","journal-title":"Platelets"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/j.1537-2995.2012.03668.x","article-title":"Antimicrobial activity of platelet (PLT)-poor plasma, PLT-rich plasma, PLT gel, and solvent\/detergent-treated PLT lysate biomaterials against wound bacteria","volume":"53","author":"Burnouf","year":"2013","journal-title":"Transfusion"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1016\/j.jcyt.2014.06.003","article-title":"Platelet-rich plasma affects bacterial growth in vitro","volume":"16","author":"Mariani","year":"2014","journal-title":"Cytotherapy"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00068-018-0957-0","article-title":"Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: In vitro antibacterial activity study","volume":"45","author":"Yenilmez","year":"2019","journal-title":"Eur. J. Trauma Emerg. Surg."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Yang, S.C., Lin, C.F., Alshetaili, A., Aljuffali, I.A., Chien, M.Y., and Fang, J.Y. (2023). Combining the dual antibacterial and regenerative activities of platelet-rich plasma with \u03b2-lactams to mitigate MRSA-infected skin wounds. Biomed. Pharmacother., 165.","DOI":"10.1016\/j.biopha.2023.115017"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.chroma.2015.12.033","article-title":"Capillary electrophoresis coupled with inductively coupled mass spectrometry as an alternative to cloud point extraction-based methods for rapid quantification of silver ions and surface coated silver nanoparticles","volume":"1429","author":"Qu","year":"2016","journal-title":"J. Chromatogr. A."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"De Mori, A., Hafidh, M., Mele, N., Yusuf, R., Cerri, G., Gavini, E., Tozzi, G., Barbu, E., Conconi, M., and Draheim, R.R. (2019). Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications. Pharmaceutics, 11.","DOI":"10.20944\/preprints201902.0157.v1"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/jopr.12316","article-title":"Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD-Coupled Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for Peri-Implantitis","volume":"25","author":"Diniz","year":"2016","journal-title":"J. Prosthodont."},{"key":"ref_66","first-page":"13","article-title":"Tissue Reaction and Biocompatibility of Implanted Mineral Trioxide Aggregate with Silver Nanoparticles in a Rat Model","volume":"11","author":"Zand","year":"2016","journal-title":"Iran Endod. J."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Castiglioni, S., Cazzaniga, A., Locatelli, L., and Maier, J.A.M. (2017). Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells. Nanomaterials, 7.","DOI":"10.3390\/nano7060124"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1080\/01480545.2021.1950167","article-title":"Distribution, metabolism, excretion, and toxicity of implanted silver: A review","volume":"45","author":"Hadrup","year":"2022","journal-title":"Drug Chem. Toxicol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1080\/21691401.2018.1552594","article-title":"Effect of exposure of osteoblast-like cells to low-dose silver nanoparticles: Uptake, retention and osteogenic activity","volume":"47","author":"Xie","year":"2019","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_70","first-page":"729","article-title":"Platelet-rich plasma inhibits RANKL-induced osteoclast differentiation through activation of Wnt pathway during bone remodeling","volume":"41","author":"Wang","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1111\/1469-0691.12651","article-title":"Antimicrobial susceptibility testing in biofilm-growing bacteria","volume":"20","author":"Oliver","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13756-019-0533-3","article-title":"Antibiotics versus biofilm: An emerging battleground in microbial communities","volume":"8","author":"Sharma","year":"2019","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.jgar.2019.11.012","article-title":"Non-antibiotic antimicrobial agents to combat biofilm-forming bacteria","volume":"21","author":"Cao","year":"2020","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Lichtenberg, M., Coenye, T., Parsek, M.R., Bjarnsholt, T., and Jakobsen, T.H. (2023). What\u2019s in a name? Characteristics of clinical biofilms. FEMS Microbiol. Rev., 47.","DOI":"10.1093\/femsre\/fuad050"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2556","DOI":"10.1093\/jac\/dkr350","article-title":"Combinatorial activities of ionic silver and sodium hexametaphosphate against microorganisms associated with chronic wounds","volume":"66","author":"Humphreys","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Sharma, B.K., Saha, A., Rahaman, L., Bhattacharjee, S., and Tribedi, P. (2015). Silver inhibits the biofilm formation of Pseudomonas aeruginosa. Adv. Microbiol., 5.","DOI":"10.4236\/aim.2015.510070"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.ijantimicag.2014.09.008","article-title":"Ag5IO6: Novel antibiofilm activity of a silver compound with application to medical devices","volume":"45","author":"Incani","year":"2015","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1021\/acschembio.7b01000","article-title":"Quorum Sensing Signals Form Complexes with Ag+ and Cu2+ Cations","volume":"13","author":"McGivney","year":"2018","journal-title":"ACS Chem. Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3985","DOI":"10.1021\/acsabm.0c01485","article-title":"Release strategies of silver ions from materials for bacterial killing","volume":"4","author":"Xu","year":"2021","journal-title":"ACS Appl. Bio. Mater."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1080\/08927014.2021.1939019","article-title":"Molecular docking, quorum quenching effect, antibiofilm activity and safety profile of silver-complexed sulfonamide on Pseudomonas aeruginosa","volume":"37","author":"Siqueira","year":"2021","journal-title":"Biofouling"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.dental.2015.01.007","article-title":"Cytotoxicity of dimethyl sulfoxide (DMSO) in direct contact with odontoblast-like cells","volume":"31","author":"Hebling","year":"2015","journal-title":"Dent. Mater."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"347","DOI":"10.3109\/10520298309066811","article-title":"A New Method Using Hexamethyldisilazane for Preparation of Soft Insect Tissues for Scanning Electron Microscopy","volume":"58","author":"Nation","year":"1983","journal-title":"Stain. Technol."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Bosetti, M., Boffano, P., Marchetti, A., Leigheb, M., Colli, M., and Brucoli, M. (2019). The Number of Platelets in Patient\u2019s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010139"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Ceresa, C., Tessarolo, F., Maniglio, D., Tambone, E., Carmagnola, I., Fedeli, E., Caola, I., Nollo, G., Chiono, V., and Allegrone, G. (2019). Medical-Grade Silicone Coated with Rhamnolipid R89 Is Effective against Staphylococcus spp. Biofilms. Molecules, 24.","DOI":"10.3390\/molecules24213843"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Ceresa, C., Rinaldi, M., Tessarolo, F., Maniglio, D., Fedeli, E., Tambone, E., Caciagli, P., Banat, I.M., Diaz De Rienzo, M.A., and Fracchia, L. (2021). Inhibitory Effects of Lipopeptides and Glycolipids on C. albicans-Staphylococcus spp. Dual-Species Biofilms. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.545654"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"7572","DOI":"10.1016\/j.biomaterials.2005.05.033","article-title":"Effects of phosphatidylserine coatings on titanium on inflammatory cells and cell-induced mineralisation in vitro","volume":"26","author":"Bosetti","year":"2005","journal-title":"Biomaterials"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s10856-007-2309-1","article-title":"Cell behaviour on phospholipids-coated surfaces","volume":"18","author":"Bosetti","year":"2007","journal-title":"J. Mater. Sci. Mater. Med."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/9\/4842\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:35:54Z","timestamp":1760106954000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/9\/4842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,29]]},"references-count":87,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["ijms25094842"],"URL":"https:\/\/doi.org\/10.3390\/ijms25094842","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,29]]}}}