{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:20:12Z","timestamp":1785367212424,"version":"3.55.0"},"reference-count":46,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Romania\u2019s National Recovery and Resilience Plan","award":["PNRR-III-C9-2022- I8"],"award-info":[{"award-number":["PNRR-III-C9-2022- I8"]}]},{"name":"Romania\u2019s National Recovery and Resilience Plan","award":["CF 93\/15.11.2022"],"award-info":[{"award-number":["CF 93\/15.11.2022"]}]},{"name":"Romania\u2019s National Recovery and Resilience Plan","award":["760063\/23.05.2023"],"award-info":[{"award-number":["760063\/23.05.2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Despite neutrophil involvement in inflammation and tissue repair, little is understood about their inflammatory status in acute coronary syndrome (ACS) patients with poor outcomes. Hence, we investigated the potential correlation between neutrophil inflammatory markers and the prognosis of ACS patients with\/without diabetes and explored whether neutrophils demonstrate a unique inflammatory phenotype in patients experiencing an adverse in-hospital outcome. The study enrolled 229 ACS patients with or without diabetes. Poor evolution was defined as either death, left ventricular ejection fraction (LVEF) &lt;40%, Killip Class 3\/4, ventricular arrhythmias, or mechanical complications. Univariate and multivariate analyses were employed to identify clinical and paraclinical factors associated with in-hospital outcomes. Neutrophils isolated from fresh blood were investigated using qPCR, Western blot, enzymatic assay, and immunofluorescence. Poor evolution post-myocardial infarction (MI) was associated with increased number, activity, and inflammatory status of neutrophils, as indicated by significant increase of Erythrocyte Sedimentation Rate (ESR), C-reactive protein (CRP), fibrinogen, interleukin-1\u03b2 (IL-1\u03b2), and, interleukin-6 (IL-6). Among the patients with complicated evolution, neutrophil activity had an important prognosis value for diabetics. Neutrophils from patients with unfavorable evolution revealed a pro-inflammatory phenotype with increased expression of CCL3, IL-1\u03b2, interleukin-18 (IL-18), S100A9, intracellular cell adhesion molecule-1 (ICAM-1), matrix metalloprotease (MMP-9), of molecules essential in reactive oxygen species (ROS) production p22phox and Nox2, and increased capacity to form neutrophil extracellular traps. Inflammation is associated with adverse short-term prognosis in acute ACS, and inflammatory biomarkers exhibit greater specificity in predicting short-term outcomes in diabetics. Moreover, neutrophils from patients with unfavorable evolution exhibit distinct inflammatory patterns, suggesting that alterations in the innate immune response in this subgroup may exert detrimental effects on disease progression.<\/jats:p>","DOI":"10.3390\/ijms25105107","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T12:27:11Z","timestamp":1715171231000},"page":"5107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9724-7143","authenticated-orcid":false,"given":"Elena","family":"Barbu","sequence":"first","affiliation":[{"name":"Department of Cardiology, Elias Emergency Hospital, 011461, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4709-7124","authenticated-orcid":false,"given":"Andreea Cristina","family":"Mihaila","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana-Maria","family":"Gan","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letitia","family":"Ciortan","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Razvan Daniel","family":"Macarie","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica Madalina","family":"Tucureanu","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru","family":"Filippi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 020021 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andra Ioana","family":"Stoenescu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Elias Emergency Hospital, 011461, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanita Victoria","family":"Petrea","sequence":"additional","affiliation":[{"name":"Nephrology Hospital Dr. Carol Davila, 010731 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2077-0400","authenticated-orcid":false,"given":"Maya","family":"Simionescu","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4949-3064","authenticated-orcid":false,"given":"Serban Mihai","family":"Balanescu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Elias Emergency Hospital, 011461, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5748-5641","authenticated-orcid":false,"given":"Elena","family":"Butoi","sequence":"additional","affiliation":[{"name":"Biopathology and Therapy of Inflammation, Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1016\/j.amjcard.2013.01.287","article-title":"Effects of interleukin-1 blockade with anakinra on adverse cardiac remodeling and heart failure after acute myocardial infarction [from the Virginia Commonwealth University-Anakinra Remodeling Trial (2) (VCU-ART2) pilot study]","volume":"111","author":"Abbate","year":"2013","journal-title":"Am. 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