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Envenomation by Bothrops genus snakes leads to severe manifestations due to proteolytic enzymes. While the antibothropic serum produced by the Butantan Institute saves lives, its efficacy is limited as it fails to neutralize certain serine proteases. Hence, developing new-generation antivenoms, like monoclonal antibodies, is crucial. This study aimed to explore the inhibitory potential of synthetic peptides homologous to the CDR3 regions of a monoclonal antibody targeting a snake venom thrombin-like enzyme (SVTLE) from B. atrox venom. Five synthetic peptides were studied, all stable against hydrolysis by venoms and serine proteases. Impressively, four peptides demonstrated uncompetitive SVTLE inhibition, with Ki values ranging from 10\u22126 to 10\u22127 M. These findings underscore the potential of short peptides homologous to CDR3 regions in blocking snake venom toxins, suggesting their promise as the basis for new-generation antivenoms. Thus, this study offers potential advancements in combatting snakebites, addressing a critical public health challenge in tropical and subtropical regions.<\/jats:p>","DOI":"10.3390\/ijms25105181","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T03:21:04Z","timestamp":1715311264000},"page":"5181","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of the Inhibitory Potential of Synthetic Peptides Homologous to CDR3 Regions of a Monoclonal Antibody against Bothropic Venom Serine Proteases"],"prefix":"10.3390","volume":"25","author":[{"given":"Lucas Yuri","family":"Saladini","sequence":"first","affiliation":[{"name":"Laboratory of Structure and Function of Biomolecules, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Jorge","family":"Magalh\u00e3es-Junior","sequence":"additional","affiliation":[{"name":"Laboratory of Immunochemistry, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiane Castilho Fernandes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Structure and Function of Biomolecules, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila Gon\u00e7alves Coutinho","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Structure and Function of Biomolecules, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0565-9018","authenticated-orcid":false,"given":"Roberto Tadashi","family":"Kodama","sequence":"additional","affiliation":[{"name":"Laboratory of Structure and Function of Biomolecules, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9961-2642","authenticated-orcid":false,"given":"Lais","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory of Structure and Function of Biomolecules, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2410-0562","authenticated-orcid":false,"given":"Milton Yutaka","family":"Nishiyama-Jr","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick Jack","family":"Spencer","sequence":"additional","affiliation":[{"name":"Biotechnology Center, Nuclear and Energy Research Institute (IPEN\/CNEN\/SP), S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilmar Dias","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Immunochemistry, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9582-4699","authenticated-orcid":false,"given":"Fernanda Calheta Vieira","family":"Portaro","sequence":"additional","affiliation":[{"name":"Laboratory of Structure and Function of Biomolecules, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2021, October 12). 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