{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T09:13:44Z","timestamp":1777626824932,"version":"3.51.4"},"reference-count":60,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French Ministry of Agriculture and Food Sovereignty"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The emerging heteropathotype shigatoxigenic (STEC) and extra-intestinal pathogenic Escherichia coli (ExPEC) O80:H2 has been the second leading cause of pediatric HUS in France since the mid-2010s. In contrast with other highly pathogenic STEC serotypes, for which ruminants have clearly been identified as the main human infection source, this heteropathotype\u2019s reservoir remains unknown. In this context, we describe for the first time the isolation of seven STEC O80:H2 strains from healthy cattle on a single cattle farm in France. This study aimed at (i) characterizing the genome and (ii) investigating the phylogenetic positions of these O80:H2 STEC strains. The virulomes, resistomes, and phylogenetic positions of the seven bovine isolates were investigated using in silico typing tools, antimicrobial susceptibility testing and cgMLST analysis after short-read whole genome sequencing (WGS). One representative isolate (A13P112V1) was also subjected to long-read sequencing. The seven isolates possessed ExPEC-related virulence genes on a pR444_A-like mosaic plasmid, previously described in strain RDEx444 and known to confer multi-drug resistance. All isolates were clonally related and clustered with human clinical strains from France and Switzerland with a range of locus differences of only one to five. In conclusion, our findings suggest that healthy cattle in France could potentially act as a reservoir of the STEC-ExPEC O80:H2 pathotype.<\/jats:p>","DOI":"10.3390\/ijms25105428","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T06:44:31Z","timestamp":1715841871000},"page":"5428","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["First Isolation of the Heteropathotype Shiga Toxin-Producing and Extra-Intestinal Pathogenic (STEC-ExPEC) E. coli O80:H2 in French Healthy Cattle: Genomic Characterization and Phylogenetic Position"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4494-7011","authenticated-orcid":false,"given":"Nathan","family":"Soleau","sequence":"first","affiliation":[{"name":"\u2018Bacterial Opportunistic Pathogens and Environment\u2019 (BPOE) Research Team, UMR5557 Ecologie Microbienne Lyon, CNRS (National Center of Scientific Research), VetAgro Sup, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Ganet","sequence":"additional","affiliation":[{"name":"\u2018Bacterial Opportunistic Pathogens and Environment\u2019 (BPOE) Research Team, UMR5557 Ecologie Microbienne Lyon, CNRS (National Center of Scientific Research), VetAgro Sup, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"},{"name":"Laboratoire d\u2019\u00c9tude des Microorganismes Alimentaires Pathog\u00e8nes\u2013French National Reference Laboratory for <i>Escherichia coli<\/i> Including STEC (NRL-STEC), VetAgro Sup\u2013Campus V\u00e9t\u00e9rinaire, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phanie","family":"Werlen","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019\u00c9tude des Microorganismes Alimentaires Pathog\u00e8nes\u2013French National Reference Laboratory for <i>Escherichia coli<\/i> Including STEC (NRL-STEC), VetAgro Sup\u2013Campus V\u00e9t\u00e9rinaire, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lia","family":"Collignon","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019\u00c9tude des Microorganismes Alimentaires Pathog\u00e8nes\u2013French National Reference Laboratory for <i>Escherichia coli<\/i> Including STEC (NRL-STEC), VetAgro Sup\u2013Campus V\u00e9t\u00e9rinaire, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6484-0696","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Cointe","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Centre National de R\u00e9f\u00e9rence <i>Escherichia coli<\/i>, AP-HP, H\u00f4pital Robert-Debr\u00e9, Universit\u00e9 Paris-Cit\u00e9, IAME, UMR 1137, INSERM, 75018 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6447-6874","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bonacorsi","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Centre National de R\u00e9f\u00e9rence <i>Escherichia coli<\/i>, AP-HP, H\u00f4pital Robert-Debr\u00e9, Universit\u00e9 Paris-Cit\u00e9, IAME, UMR 1137, INSERM, 75018 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"Sergentet","sequence":"additional","affiliation":[{"name":"\u2018Bacterial Opportunistic Pathogens and Environment\u2019 (BPOE) Research Team, UMR5557 Ecologie Microbienne Lyon, CNRS (National Center of Scientific Research), VetAgro Sup, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"},{"name":"Laboratoire d\u2019\u00c9tude des Microorganismes Alimentaires Pathog\u00e8nes\u2013French National Reference Laboratory for <i>Escherichia coli<\/i> Including STEC (NRL-STEC), VetAgro Sup\u2013Campus V\u00e9t\u00e9rinaire, Universit\u00e9 de Lyon, Marcy-l\u2019\u00c9toile, 69280 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1128\/IAI.01182-10","article-title":"Shiga Toxin Subtypes Display Dramatic Differences in Potency","volume":"79","author":"Fuller","year":"2011","journal-title":"Infect. 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