{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T06:28:32Z","timestamp":1781591312888,"version":"3.54.5"},"reference-count":30,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T00:00:00Z","timestamp":1716508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI17\/00123"],"award-info":[{"award-number":["PI17\/00123"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI20\/01819"],"award-info":[{"award-number":["PI20\/01819"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CB06\/02\/0073"],"award-info":[{"award-number":["CB06\/02\/0073"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["2021SGR01029"],"award-info":[{"award-number":["2021SGR01029"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["2021SGR1354"],"award-info":[{"award-number":["2021SGR1354"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI18\/00244"],"award-info":[{"award-number":["FI18\/00244"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FPU2020-02907"],"award-info":[{"award-number":["FPU2020-02907"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CIBERESP","award":["PI17\/00123"],"award-info":[{"award-number":["PI17\/00123"]}]},{"name":"CIBERESP","award":["PI20\/01819"],"award-info":[{"award-number":["PI20\/01819"]}]},{"name":"CIBERESP","award":["CB06\/02\/0073"],"award-info":[{"award-number":["CB06\/02\/0073"]}]},{"name":"CIBERESP","award":["2021SGR01029"],"award-info":[{"award-number":["2021SGR01029"]}]},{"name":"CIBERESP","award":["2021SGR1354"],"award-info":[{"award-number":["2021SGR1354"]}]},{"name":"CIBERESP","award":["FI18\/00244"],"award-info":[{"award-number":["FI18\/00244"]}]},{"name":"CIBERESP","award":["FPU2020-02907"],"award-info":[{"award-number":["FPU2020-02907"]}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["PI17\/00123"],"award-info":[{"award-number":["PI17\/00123"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["PI20\/01819"],"award-info":[{"award-number":["PI20\/01819"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["CB06\/02\/0073"],"award-info":[{"award-number":["CB06\/02\/0073"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["2021SGR01029"],"award-info":[{"award-number":["2021SGR01029"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["2021SGR1354"],"award-info":[{"award-number":["2021SGR1354"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["FI18\/00244"],"award-info":[{"award-number":["FI18\/00244"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"The Secretariat for Universities and Research of the Department of Business and Knowledge of the Government of Catalonia","doi-asserted-by":"publisher","award":["FPU2020-02907"],"award-info":[{"award-number":["FPU2020-02907"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI17\/00123"],"award-info":[{"award-number":["PI17\/00123"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI20\/01819"],"award-info":[{"award-number":["PI20\/01819"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CB06\/02\/0073"],"award-info":[{"award-number":["CB06\/02\/0073"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["2021SGR01029"],"award-info":[{"award-number":["2021SGR01029"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["2021SGR1354"],"award-info":[{"award-number":["2021SGR1354"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI18\/00244"],"award-info":[{"award-number":["FI18\/00244"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"the Contratos Predoctorales de Formaci\u00f3n en Investigaci\u00f3n en salud (PFIS) program from the Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FPU2020-02907"],"award-info":[{"award-number":["FPU2020-02907"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["PI17\/00123"],"award-info":[{"award-number":["PI17\/00123"]}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["PI20\/01819"],"award-info":[{"award-number":["PI20\/01819"]}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["CB06\/02\/0073"],"award-info":[{"award-number":["CB06\/02\/0073"]}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["2021SGR01029"],"award-info":[{"award-number":["2021SGR01029"]}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["2021SGR1354"],"award-info":[{"award-number":["2021SGR1354"]}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["FI18\/00244"],"award-info":[{"award-number":["FI18\/00244"]}]},{"name":"The Formaci\u00f3n de profesorado universitario (FPU) program from the Spanish Ministerio de Universidades","award":["FPU2020-02907"],"award-info":[{"award-number":["FPU2020-02907"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Recent studies have revealed the impact of human papillomavirus (HPV) infections on the cervicovaginal microbiome; however, few have explored the utility of self-collected specimens (SCS) for microbiome detection, obtained using standardised methods for HPV testing. Here, we present a proof-of-concept analysis utilising Oxford Nanopore sequencing of the 16S rRNA gene in paired samples collected either by the patient using an Evalyn Brush or collected by a physician using liquid-based cytology (LBC). We found no significant differences in the \u03b1-diversity estimates between the SCS and LBC samples. Similarly, when analysing \u03b2-diversity, we observed a close grouping of paired samples, indicating that both collection methods detected the same microbiome features. The identification of genera and Lactobacillus species in each sample allowed for their classification into community state types (CSTs). Notably, paired samples had the same CST, while HPV-positive and -negative samples belonged to distinct CSTs. As previously described in other studies, HPV-positive samples exhibited heightened bacterial diversity, reduced Lactobacillus abundance, and an increase in genera like Sneathia or Dialister. Altogether, this study showed comparable results between the SCS and LBC samples, underscoring the potential of self-sampling for analysing the microbiome composition in cervicovaginal samples initially collected for HPV testing in the context of cervical cancer screening.<\/jats:p>","DOI":"10.3390\/ijms25115736","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T11:17:52Z","timestamp":1716549472000},"page":"5736","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Proof of Concept Study: Comparability of Microbiome Diversity in Self- and Physician-Collected HPV-Positive and HPV-Negative Cervicovaginal Samples"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1772-1923","authenticated-orcid":false,"given":"Laura","family":"Asensio-Puig","sequence":"first","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology\u2014Bellvitge Biomedical Research Institute (IDIBELL), 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Programa de Doctorat en Biomedicina, Universitat de Barcelona (UB), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0432-5078","authenticated-orcid":false,"given":"\u00c1lvaro","family":"de Andr\u00e9s-Pablo","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology\u2014Bellvitge Biomedical Research Institute (IDIBELL), 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Programa de Doctorat en Biomedicina, Universitat de Barcelona (UB), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olfat","family":"Khannous-Lleiffe","sequence":"additional","affiliation":[{"name":"Barcelona Supercomputing Centre (BSC-CNS), 08034 Barcelona, Spain"},{"name":"Institute for Research in Biomedicine (IRB), 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Ib\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology\u2014Bellvitge Biomedical Research Institute (IDIBELL), 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP CB06\/02\/0073), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Acera","sequence":"additional","affiliation":[{"name":"Atenci\u00f3 a la Salut Sexual i Reproductiva (ASSIR), SAP Cerdanyola-Ripollet, Institut Catal\u00e0 de la Salut, 08291 Ripollet, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"de Sanjos\u00e9","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP CB06\/02\/0073), 28029 Madrid, Spain"},{"name":"ISGlobal, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0019-1735","authenticated-orcid":false,"given":"Toni","family":"Gabald\u00f3n","sequence":"additional","affiliation":[{"name":"Barcelona Supercomputing Centre (BSC-CNS), 08034 Barcelona, Spain"},{"name":"Institute for Research in Biomedicine (IRB), 08028 Barcelona, Spain"},{"name":"Catalan Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain"},{"name":"CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Alemany","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology\u2014Bellvitge Biomedical Research Institute (IDIBELL), 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP CB06\/02\/0073), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Bruni","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology\u2014Bellvitge Biomedical Research Institute (IDIBELL), 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP CB06\/02\/0073), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miquel \u00c0ngel","family":"Pav\u00f3n","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology\u2014Bellvitge Biomedical Research Institute (IDIBELL), 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP CB06\/02\/0073), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F","article-title":"Human papillomavirus is a necessary cause of invasive cervical cancer worldwide","volume":"189","author":"Walboomers","year":"1999","journal-title":"J. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"918","DOI":"10.15585\/mmwr.mm6733a2","article-title":"Trends in human papillomavirus\u2014Associated cancers? United States, 1999\u20132015","volume":"67","author":"Henley","year":"2018","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1002\/ijc.24010","article-title":"Guidelines for human papillomavirus DNA test requirements for primary cervical cancer screening in women 30 years and older","volume":"124","author":"Meijer","year":"2009","journal-title":"Int. J. Cancer"},{"key":"ref_4","first-page":"CD008587","article-title":"Cytology versus HPV testing for cervical cancer screening in the general population","volume":"8","author":"Koliopoulos","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_5","first-page":"20","article-title":"The possible effects on socio-economic inequalities of introducing HPV testing as primary test in cervical cancer screening programs","volume":"4","author":"Baldacchini","year":"2014","journal-title":"Front. Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"106900","DOI":"10.1016\/j.ypmed.2021.106900","article-title":"Worldwide use of HPV self-sampling for cervical cancer screening","volume":"154","author":"Serrano","year":"2022","journal-title":"Prev. Med."},{"key":"ref_7","unstructured":"WHO (2021). WHO Guideline on Self-Care Interventions for Health and Well-Being, World Health Organization."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e003743","DOI":"10.1136\/bmjgh-2020-003743","article-title":"HPV self-sampling for cervical cancer screening: A systematic review of values and preferences","volume":"6","author":"Nishimura","year":"2021","journal-title":"BMJ Glob. Health"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"L\u00e4sche, M., Urban, H., Gallwas, J., and Gr\u00fcndker, C. (2021). HPV and other microbiota; who\u2019s good and who\u2019s bad: Effects of the microbial environment on the development of cervical cancer\u2014A non-systematic review. Cells, 10.","DOI":"10.3390\/cells10030714"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4680","DOI":"10.1073\/pnas.1002611107","article-title":"Vaginal microbiome of reproductive-age women","volume":"108","author":"Ravel","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Gardella, B., Pasquali, M.F., La Verde, M., Cianci, S., Torella, M., and Dominoni, M. (2022). The complex interplay between vaginal microbiota, HPV infection, and immunological microenvironment in cervical intraepithelial neoplasia: A literature review. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137174"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Audirac-Chalifour, A., Torres-Poveda, K., Bahena-Rom\u00e1n, M., T\u00e9llez-Sosa, J., Mart\u00ednez-Barnetche, J., Cortina-Ceballos, B., L\u00f3pez-Estrada, G., Delgado-Romero, K., Burguete-Garc\u00eda, A.I., and Cant\u00fa, D. (2016). Cervical microbiome and cytokine profile at various stages of cervical cancer: A pilot study. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0153274"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Molina, M.A., Andralojc, K.M., Huynen, M.A., Leenders, W.P., and Melchers, W.J. (2022). In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling. NPJ Biofilms Microbiomes, 8.","DOI":"10.1038\/s41522-022-00336-6"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/s00248-006-9199-5","article-title":"Genotypic microbial community profiling: A critical technical review","volume":"54","author":"Nocker","year":"2007","journal-title":"Microb. Ecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"k4823","DOI":"10.1136\/bmj.k4823","article-title":"Detecting cervical precancer and reaching underscreened women by using HPV testing on self samples: Updated meta-analyses","volume":"363","author":"Arbyn","year":"2018","journal-title":"BMJ"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kamath Mulki, A., and Withers, M. (2021). Human Papilloma Virus self-sampling performance in low-and middle-income countries. BMC Womens Health, 21.","DOI":"10.1186\/s12905-020-01158-4"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1128\/JCM.01710-09","article-title":"Comparison of self-collected and physician-collected vaginal swabs for microbiome analysis","volume":"48","author":"Forney","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107571","DOI":"10.1016\/j.ypmed.2023.107571","article-title":"HPV self-sampling among cervical cancer screening users in Spain: A randomized clinical trial of on-site training to increase the acceptability","volume":"173","author":"Roura","year":"2023","journal-title":"Prev. Med."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Turner, F., Drury, J., Hapangama, D.K., and Tempest, N. (2023). Menstrual Tampons Are Reliable and Acceptable Tools to Self-Collect Vaginal Microbiome Samples. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241814121"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wylie, K.M., Blankenship, S.A., Tuuli, M.G., Macones, G.A., and Stout, M.J. (2018). Evaluation of patient-versus provider-collected vaginal swabs for microbiome analysis during pregnancy. BMC Res. Notes, 11.","DOI":"10.1186\/s13104-018-3809-4"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fang, B., Li, Q., Wan, Z., OuYang, Z., and Zhang, Q. (2022). Exploring the association between cervical microbiota and HR-HPV infection based on 16S rRNA gene and metagenomic sequencing. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.922554"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Usyk, M., Zolnik, C.P., Castle, P.E., Porras, C., Herrero, R., Gradissimo, A., Gonzalez, P., Safaeian, M., Schiffman, M., and Burk, R.D. (2020). Cervicovaginal microbiome and natural history of HPV in a longitudinal study. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008376"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1038\/s41467-020-15856-y","article-title":"The vaginal microbiota associates with the regression of untreated cervical intraepithelial neoplasia 2 lesions","volume":"11","author":"Mitra","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_24","first-page":"e000132","article-title":"Completing bacterial genome assemblies with multiplex MinION sequencing","volume":"3","author":"Wick","year":"2017","journal-title":"Microb. Genom."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1093\/bioinformatics\/bty149","article-title":"NanoPack: Visualizing and processing long-read sequencing data","volume":"34","author":"Schultz","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_26","unstructured":"Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data, Babraham Institute."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Wood, D.E., Lu, J., and Langmead, B. (2019). Improved metagenomic analysis with Kraken 2. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1891-0"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Quast","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"France, M.T., Ma, B., Gajer, P., Brown, S., Humphrys, M.S., Holm, J.B., Waetjen, L.E., Brotman, R.M., and Ravel, J. (2020). VALENCIA: A nearest centroid classification method for vaginal microbial communities based on composition. Microbiome, 8.","DOI":"10.1186\/s40168-020-00934-6"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/11\/5736\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:48:26Z","timestamp":1760107706000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/11\/5736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,24]]},"references-count":30,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["ijms25115736"],"URL":"https:\/\/doi.org\/10.3390\/ijms25115736","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,24]]}}}