{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:08:02Z","timestamp":1760144882248,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,26]],"date-time":"2024-05-26T00:00:00Z","timestamp":1716681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPESP","award":["2020\/07040-1","2022\/00205-0","2020\/13139-0","2016\/19391-8"],"award-info":[{"award-number":["2020\/07040-1","2022\/00205-0","2020\/13139-0","2016\/19391-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Tetanus disease, caused by C. tetani, starts with wounds or mucous layer contact. Prevented by vaccination, the lack of booster shots throughout life requires prophylactic treatment in case of accidents. The incidence of tetanus is high in underdeveloped countries, requiring the administration of antitetanus antibodies, usually derived from immunized horses or humans. Heterologous sera represent risks such as serum sickness. Human sera can carry unknown viruses. In the search for human monoclonal antibodies (mAbs) against TeNT (Tetanus Neurotoxin), we previously identified a panel of mAbs derived from B-cell sorting, selecting two nonrelated ones that binded to the C-terminal domain of TeNT (HCR\/T), inhibiting its interaction with the cellular receptor ganglioside GT1b. Here, we present the results of cellular assays and molecular docking tools. TeNT internalization in neurons is prevented by more than 50% in neonatal rat spinal cord cells, determined by quantitative analysis of immunofluorescence punctate staining of Alexa Fluor 647 conjugated to TeNT. We also confirmed the mediator role of the Synaptic Vesicle Glycoprotein II (SV2) in TeNT endocytosis. The molecular docking assays to predict potential TeNT epitopes showed the binding of both antibodies to the HCR\/T domain. A higher incidence was found between N1153 and W1297 when evaluating candidate residues for conformational epitope.<\/jats:p>","DOI":"10.3390\/ijms25115788","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T10:14:07Z","timestamp":1716804847000},"page":"5788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Dual Strategy\u2014In Vitro and In Silico\u2014To Evaluate Human Antitetanus mAbs Addressing Their Potential Protective Action on TeNT Endocytosis in Primary Rat Neuronal Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7945-8070","authenticated-orcid":false,"given":"Cau\u00e3 Pacheco","family":"Lima","sequence":"first","affiliation":[{"name":"Laboratory of Biopharmaceuticals, Butantan Institute, Sao Paulo 05503-900, Brazil"},{"name":"Interunits Graduate Program in Biotechnology, University of Sao Paulo, Sao Paulo 05508-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1406-2456","authenticated-orcid":false,"given":"Gabriela Massaro","family":"Barreiros","sequence":"additional","affiliation":[{"name":"Laboratory of Biopharmaceuticals, Butantan Institute, Sao Paulo 05503-900, Brazil"},{"name":"Interunits Graduate Program in Biotechnology, University of Sao Paulo, Sao Paulo 05508-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4798-7752","authenticated-orcid":false,"given":"Adriele Silva Alves","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Biopharmaceuticals, Butantan Institute, Sao Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Medina","family":"de Souza","sequence":"additional","affiliation":[{"name":"CENTD\u2014Centre of Excellence in New Target Discovery, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1152-7425","authenticated-orcid":false,"given":"Tania Maria","family":"Manieri","sequence":"additional","affiliation":[{"name":"Laboratory of Biopharmaceuticals, Butantan Institute, Sao Paulo 05503-900, Brazil"},{"name":"CeRDI\u2014Center for Research and Development in Immunobiologicals, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0650-7764","authenticated-orcid":false,"given":"Ana Maria","family":"Moro","sequence":"additional","affiliation":[{"name":"Laboratory of Biopharmaceuticals, Butantan Institute, Sao Paulo 05503-900, Brazil"},{"name":"CeRDI\u2014Center for Research and Development in Immunobiologicals, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,26]]},"reference":[{"key":"ref_1","first-page":"e01007","article-title":"Advances and challenges in therapeutic monoclonal antibodies drug development","volume":"54","author":"Quintilio","year":"2018","journal-title":"Braz. 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Model."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/11\/5788\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:49:00Z","timestamp":1760107740000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/11\/5788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,26]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["ijms25115788"],"URL":"https:\/\/doi.org\/10.3390\/ijms25115788","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,5,26]]}}}