{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:27:14Z","timestamp":1790011634861,"version":"4.0.1"},"reference-count":50,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Salerno","award":["ORSA22727"],"award-info":[{"award-number":["ORSA22727"]}]},{"name":"University of Salerno","award":["P2022CKMPW"],"award-info":[{"award-number":["P2022CKMPW"]}]},{"name":"Ministero dell\u2019Universit\u00e0 e della Ricerca (MUR)","award":["ORSA22727"],"award-info":[{"award-number":["ORSA22727"]}]},{"name":"Ministero dell\u2019Universit\u00e0 e della Ricerca (MUR)","award":["P2022CKMPW"],"award-info":[{"award-number":["P2022CKMPW"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This research focuses on the target deconvolution of the natural compound myrianthic acid, a triterpenoid characterized by an ursane skeleton isolated from the roots of Myrianthus arboreus and from Oenothera maritima Nutt. (Onagraceae), using MS-based chemical proteomic techniques. Application of drug affinity responsive target stability (DARTS) and targeted-limited proteolysis coupled to mass spectrometry (t-LiP-MS) led to the identification of the enzyme fatty acid synthase (FAS) as an interesting macromolecular counterpart of myrianthic acid. This result, confirmed by comparison with the natural ursolic acid, was thoroughly investigated and validated in silico by molecular docking, which gave a precise picture of the interactions in the MA\/FAS complex. Moreover, biological assays showcased the inhibitory activity of myrianthic acid against the FAS enzyme, most likely related to its antiproliferative activity towards tumor cells. Given the significance of FAS in specific pathologies, especially cancer, the myrianthic acid structural moieties could serve as a promising reference point to start the potential development of innovative approaches in therapy.<\/jats:p>","DOI":"10.3390\/ijms25115918","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T03:38:30Z","timestamp":1716953910000},"page":"5918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Fatty Acid Synthase as Interacting Anticancer Target of the Terpenoid Myrianthic Acid Disclosed by MS-Based Proteomics Approaches"],"prefix":"10.3390","volume":"25","author":[{"given":"Alessandra","family":"Capuano","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"},{"name":"PhD Program in Drug Discovery and Development, Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4407-2781","authenticated-orcid":false,"given":"Gilda","family":"D\u2019Urso","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Gazzillo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"},{"name":"PhD Program in Drug Discovery and Development, Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5065-9717","authenticated-orcid":false,"given":"Gianluigi","family":"Lauro","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Giovanna","family":"Chini","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Territory, University of Molise, C.da Fonte Lappone, 86090 Pesche, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Valeria","family":"D\u2019Auria","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples \u201cFederico II\u201d, Via Domenico Montesano 49, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3022-1205","authenticated-orcid":false,"given":"Maria Grazia","family":"Ferraro","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Iazzetti","sequence":"additional","affiliation":[{"name":"Biochem Lab, Department of Pharmacy, School of Medicine and Surgery, University of Naples \u201cFederico II\u201d, Via Domenico Montesano 49, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5172-4573","authenticated-orcid":false,"given":"Carlo","family":"Irace","sequence":"additional","affiliation":[{"name":"Biochem Lab, Department of Pharmacy, School of Medicine and Surgery, University of Naples \u201cFederico II\u201d, Via Domenico Montesano 49, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Bifulco","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2310-9426","authenticated-orcid":false,"given":"Agostino","family":"Casapullo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1038\/nrd2410","article-title":"Target deconvolution strategies in drug discovery","volume":"6","author":"Terstappen","year":"2007","journal-title":"Nat. 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