{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T08:47:10Z","timestamp":1767084430791,"version":"build-2065373602"},"reference-count":82,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Abnormalities in sperm nuclei and chromatin can interfere with normal fertilization, embryonic development, implantation, and pregnancy. We aimed to study the impact of H2BFWT gene variants in sperm DNA on ICSI outcomes in couples undergoing ART treatment. One hundred and nineteen partners were divided into pregnant (G1) and non-pregnant (G2) groups. After semen analysis, complete DNA was extracted from purified sperm samples. The sequence of the H2BFWT gene was amplified by PCR and then subjected to Sanger sequencing. The results showed that there are three mutations in this gene: rs7885967, rs553509, and rs578953. Significant differences were shown in the distribution of alternative and reference alleles between G1 and G2 (p = 0.0004 and p = 0.0020, respectively) for rs553509 and rs578953. However, there was no association between these SNPs and the studied parameters. This study is the first to shed light on the connection between H2BFWT gene variants in sperm DNA and pregnancy after ICSI therapy. This is a pilot study, so further investigations about these gene variants at the transcriptional and translational levels will help to determine its functional consequences and to clarify the mechanism of how pregnancy can be affected by sperm DNA.<\/jats:p>","DOI":"10.3390\/ijms25116048","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T03:46:49Z","timestamp":1717127209000},"page":"6048","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["H2BFWT Variations in Sperm DNA and Its Correlation to Pregnancy"],"prefix":"10.3390","volume":"25","author":[{"given":"Houda","family":"Amor","sequence":"first","affiliation":[{"name":"Departement of Obstetrics and Gynecology, IVF Laboratory, Saarland University Clinic, 66421 Homburg, Germany"},{"name":"Departement of Obstertics and Gynecology, IVF Laboratory, Freiburg University Clinic, 79106 Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingolf","family":"Juhasz-B\u00f6ss","sequence":"additional","affiliation":[{"name":"Departement of Obstertics and Gynecology, IVF Laboratory, Freiburg University Clinic, 79106 Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5265-3626","authenticated-orcid":false,"given":"Riffat","family":"Bibi","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad 45320, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad Eid","family":"Hammadeh","sequence":"additional","affiliation":[{"name":"Departement of Obstetrics and Gynecology, IVF Laboratory, Saarland University Clinic, 66421 Homburg, Germany"},{"name":"Departement of Obstertics and Gynecology, IVF Laboratory, Freiburg University Clinic, 79106 Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter Michael","family":"Jankowski","sequence":"additional","affiliation":[{"name":"Departement of Obstetrics and Gynecology, IVF Laboratory, Saarland University Clinic, 66421 Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vavouri, T., and Lehner, B. 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