{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:22:08Z","timestamp":1792804928200,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T00:00:00Z","timestamp":1717372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ISCIII, Co-funded by European Regional Development Fund\/European Social Fund \u201cInvesting in your future\u201d","award":["CB16-10-00238"],"award-info":[{"award-number":["CB16-10-00238"]}],"id":[{"id":"https:\/\/ror.org\/048snh465","id-type":"ROR","asserted-by":"crossref"}]},{"name":"ISCIII, Co-funded by European Regional Development Fund\/European Social Fund \u201cInvesting in your future\u201d","award":["P18-RT-698"],"award-info":[{"award-number":["P18-RT-698"]}],"id":[{"id":"https:\/\/ror.org\/048snh465","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016970","name":"Junta de Andaluc\u00eda, Consejer\u00eda de Conocimiento, Investigaci\u00f3n y Universidad","doi-asserted-by":"publisher","award":["CB16-10-00238"],"award-info":[{"award-number":["CB16-10-00238"]}],"id":[{"id":"10.13039\/100016970","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016970","name":"Junta de Andaluc\u00eda, Consejer\u00eda de Conocimiento, Investigaci\u00f3n y Universidad","doi-asserted-by":"publisher","award":["P18-RT-698"],"award-info":[{"award-number":["P18-RT-698"]}],"id":[{"id":"10.13039\/100016970","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Currently, there is an increase in the aging of the population, which represents a risk factor for many diseases, including sarcopenia. Sarcopenia involves progressive loss of mass, strength, and function of the skeletal muscle. Some mechanisms include alterations in muscle structure, reduced regenerative capacity, oxidative stress, mitochondrial dysfunction, and inflammation. The zebrafish has emerged as a new model for studying skeletal muscle aging because of its numerous advantages, including histological and molecular similarity to human skeletal muscle. In this study, we used fish of 2, 10, 30, and 60 months of age. The older fish showed a higher frailty index with a value of 0.250 \u00b1 0.000 because of reduced locomotor activity and alterations in biometric measurements. We observed changes in muscle structure with a decreased number of myocytes (0.031 myocytes\/\u03bcm2 \u00b1 0.004 at 60 months) and an increase in collagen with aging up to 15% \u00b1 1.639 in the 60-month group, corresponding to alterations in the synthesis, degradation, and differentiation pathways. These changes were accompanied by mitochondrial alterations, such as a nearly 50% reduction in the number of intermyofibrillar mitochondria, 100% mitochondrial damage, and reduced mitochondrial dynamics. Overall, we demonstrated a similarity in the aging processes of muscle aging between zebrafish and mammals.<\/jats:p>","DOI":"10.3390\/ijms25116166","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T08:43:54Z","timestamp":1717577034000},"page":"6166","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Zebrafish as a Human Muscle Model for Studying Age-Dependent Sarcopenia and Frailty"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-010X","authenticated-orcid":false,"given":"Paula","family":"Aranda-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4467-6742","authenticated-orcid":false,"given":"Ramy K. A.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5593-6733","authenticated-orcid":false,"given":"Jos\u00e9","family":"Fern\u00e1ndez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5855-0449","authenticated-orcid":false,"given":"Yolanda","family":"Ram\u00edrez-Casas","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences and Medicine, Northwest University, Xi\u2019an 710069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1256-7656","authenticated-orcid":false,"given":"Germaine","family":"Escames","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Universitario San Cecilio, 18016 Granada, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9680-1560","authenticated-orcid":false,"given":"Dar\u00edo","family":"Acu\u00f1a-Castroviejo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Medicina, Departamento de Fisiolog\u00eda, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria (Ibs. Granada), Hospital Universitario San Cecilio, 18016 Granada, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"UGC de Laboratorios Cl\u00ednicos, Hospital Universitario San Cecilio, 18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2636","DOI":"10.1016\/S0140-6736(19)31138-9","article-title":"Sarcopenia","volume":"393","author":"Sayer","year":"2019","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2016.06.001","article-title":"Frailty and Sarcopenia as the Basis for the Phenotypic Manifestation of Chronic Diseases in Older Adults","volume":"50","author":"Angulo","year":"2016","journal-title":"Mol. Asp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Rogeri, P.S., Zanella, R., Martins, G.L., Garcia, M.D.A., Leite, G., Lugaresi, R., Gasparini, S.O., Sperandio, G.A., Ferreira, L.H.B., and Souza-Junior, T.P. (2021). Strategies to Prevent Sarcopenia in the Aging Process: Role of Protein Intake and Exercise. Nutrients, 14.","DOI":"10.3390\/nu14010052"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e13223","DOI":"10.1111\/acel.13223","article-title":"Animal Models of Sarcopenia","volume":"19","author":"Christian","year":"2020","journal-title":"Aging Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.exger.2016.07.007","article-title":"Identification of Morphological Markers of Sarcopenia at Early Stage of Aging in Skeletal Muscle of Mice","volume":"83","author":"Sayed","year":"2016","journal-title":"Exp. Gerontol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1093\/gerona\/gly059","article-title":"The Protective Effect of Melatonin Against Age-Associated, Sarcopenia-Dependent Tubular Aggregate Formation, Lactate Depletion, and Mitochondrial Changes","volume":"73","author":"Sayed","year":"2018","journal-title":"J. Gerontol. Ser. A Biol. Sci. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1093\/gerona\/glz079","article-title":"Lack of NLRP3 Inflammasome Activation Reduces Age-Dependent Sarcopenia and Mitochondrial Dysfunction, Favoring the Prophylactic Effect of Melatonin","volume":"74","author":"Sayed","year":"2019","journal-title":"J. Gerontol. Ser. A Biol. Sci. Med. Sci."},{"key":"ref_8","first-page":"e12912","article-title":"IMS-Bmal1\u2212\/\u2212 Mice Show Evident Signs of Sarcopenia That Are Counteracted by Exercise and Melatonin Therapies","volume":"76","author":"Sayed","year":"2023","journal-title":"J. Pineal Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19090","DOI":"10.1073\/pnas.1014523107","article-title":"CLOCK and BMAL1 Regulate MyoD and Are Necessary for Maintenance of Skeletal Muscle Phenotype and Function","volume":"107","author":"Andrews","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1101\/gad.349066.121","article-title":"BMAL1 Drives Muscle Repair through Control of Hypoxic NAD+ Regeneration in Satellite Cells","volume":"36","author":"Zhu","year":"2022","journal-title":"Genes Dev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Aranda-mart\u00ednez, P., Fern\u00e1ndez-mart\u00ednez, J., Ram\u00edrez-casas, Y., Guerra-librero, A., Rodr\u00edguez-santana, C., Escames, G., and Acu\u00f1a-castroviejo, D. (2022). The Zebrafish, an Outstanding Model for Biomedical Research in the Field of Melatonin and Human Diseases. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137438"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/S0531-5565(02)00088-8","article-title":"Life Spans and Senescent Phenotypes in Two Strains of Zebrafish (Danio Rerio)","volume":"37","author":"Gerhard","year":"2002","journal-title":"Exp. Gerontol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1002\/dvdy.22113","article-title":"Normal table of postembryonic zebrafish development: Staging by externally visible anatomy of the living fish","volume":"238","author":"Parichy","year":"2009","journal-title":"Dev. Dyn."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1016\/j.exger.2003.10.022","article-title":"Comparative aspects of zebrafish (Danio rerio) as a model for aging research","volume":"38","author":"Gerhard","year":"2003","journal-title":"Exp. Gerontol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/bdrc.20217","article-title":"The search for evolutionary developmental origins of aging in zebrafish: A novel intersection of developmental and senescence biology in the zebrafish model system","volume":"93","author":"Kishi","year":"2011","journal-title":"Birth Defects Res. C Embryo Today"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1046\/j.1474-9728.2002.00012.x","article-title":"A call to fins! Zebrafish as a gerontological model","volume":"1","author":"Gerhard","year":"2002","journal-title":"Aging Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1111\/febs.16637","article-title":"Exercise Intervention Mitigates Zebrafish Age-Related Sarcopenia via Alleviating Mitochondrial Dysfunction","volume":"290","author":"Sun","year":"2023","journal-title":"FEBS J."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Rutkove, S.B., Chen, Z.Z., Pandeya, S., Callegari, S., Mourey, T., Nagy, J.A., and Nath, A.K. (2023). Surface Electrical Impedance Myography Detects Skeletal Muscle Atrophy in Aged Wildtype Zebrafish and Aged gpr27 Knockout Zebrafish. Biomedicines, 11.","DOI":"10.3390\/biomedicines11071938"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.exger.2013.11.013","article-title":"Zebrafish (Danio rerio) as a model for the study of aging and exercise: Physical ability and trainability decrease with age","volume":"50","author":"Gilbert","year":"2014","journal-title":"Exp. Gerontol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"dmm042689","DOI":"10.1242\/dmm.042689","article-title":"Zebrafish Models of Sarcopenia","volume":"13","author":"Daya","year":"2020","journal-title":"Dis. Models Mech."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"112265","DOI":"10.1016\/j.exger.2023.112265","article-title":"Aerobic Exercise Enhances Mitochondrial Homeostasis to Counteract D-Galactose-Induced Sarcopenia in Zebrafish","volume":"180","author":"Chen","year":"2023","journal-title":"Exp. Gerontol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1093\/gerona\/gly064","article-title":"Frailty Quantified by the \u201cValencia Score\u201d as a Potential Predictor of Lifespan in Mice","volume":"73","author":"Garrido","year":"2018","journal-title":"J. Gerontol. Ser. A Biol. Sci. Med. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1096\/fj.04-2640fje","article-title":"Anabolic Signaling Deficits Underlie Amino Acid Resistance of Wasting, Aging Muscle","volume":"19","author":"Cuthbertson","year":"2005","journal-title":"FASEB J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.mad.2010.01.008","article-title":"Effects of Aging and Gender on Muscle Mass and Regulation of Akt-MTOR-P70s6k Related Signaling in the F344BN Rat Model","volume":"131","author":"Paturi","year":"2010","journal-title":"Mech. Ageing Dev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1038\/ncb1101-1014","article-title":"Akt\/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo","volume":"3","author":"Bodine","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7554\/eLife.60445","article-title":"Myogenin Is an Essential Regulator of Adult Myofibre Growth and Muscle Stem Cell Homeostasis","volume":"9","author":"Ganassi","year":"2020","journal-title":"Elife"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.mod.2013.06.001","article-title":"Control of Muscle Fibre-Type Diversity during Embryonic Development: The Zebrafish Paradigm","volume":"130","author":"Jackson","year":"2013","journal-title":"Mech. Dev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13395-014-0026-2","article-title":"The Role of Sox6 in Zebrafish Muscle Fiber Type Specification","volume":"5","author":"Jackson","year":"2015","journal-title":"Skelet Muscle"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5618","DOI":"10.1073\/pnas.0501559102","article-title":"Decline in skeletal muscle mitochondrial function with aging in humans","volume":"102","author":"Short","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/s41392-023-01547-9","article-title":"Mitochondrial dynamics in health and disease: Mechanisms and potential targets","volume":"8","author":"Chen","year":"2023","journal-title":"Signal Transduct Target. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1159\/000228892","article-title":"Zebrafish as a Genetic Model in Biological and Behavioral Gerontology: Where Development Meets Aging in Vertebrates-A Mini-Review","volume":"55","author":"Kishi","year":"2009","journal-title":"Gerontology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1111\/jgs.14952","article-title":"Weight and Body Mass Index in Old Age: Do They Still Matter?","volume":"6","author":"Harris","year":"2017","journal-title":"J. Am. Geriatr. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Buchman, A.S., Leurgans, S.E., Wang, T., Schnaider-Beeri, M., Agarwal, P., Dawe, R.J., Delbono, O., and Bennett, D.A. (2021). Motor function is the primary driver of the associations of sarcopenia and physical frailty with adverse health outcomes in community-dwelling older adults. PLoS. ONE, 16.","DOI":"10.1371\/journal.pone.0245680"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.afos.2017.08.098","article-title":"Molecular Mechanisms and Therapeutic Interventions in Sarcopenia","volume":"3","author":"Park","year":"2017","journal-title":"Osteoporos. Sarcopenia"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1002\/jcsm.13241","article-title":"Post-Translational Regulation of Muscle Growth, Muscle Aging and Sarcopenia","volume":"14","author":"Zhong","year":"2023","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Yoshida, T., and Delafontaine, P. (2020). Mechanisms of IGF-1-Mediated Regulation of Skeletal Muscle Hypertrophy and Atrophy. Cells, 9.","DOI":"10.3390\/cells9091970"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.maturitas.2016.11.006","article-title":"Inflammation and Sarcopenia: A Systematic Review and Meta-Analysis","volume":"96","author":"Bano","year":"2017","journal-title":"Maturitas"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"111544","DOI":"10.1016\/j.exger.2021.111544","article-title":"Inflammation and Sarcopenia: A Focus on Circulating Inflammatory Cytokines","volume":"154","author":"Pan","year":"2021","journal-title":"Exp. Gerontol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"111931","DOI":"10.1016\/j.exger.2022.111931","article-title":"Inflammaging: The Ground for Sarcopenia?","volume":"168","author":"Liang","year":"2022","journal-title":"Exp. Gerontol."},{"key":"ref_40","first-page":"1","article-title":"Dynamics of Myogenic Differentiation Using a Novel Myogenin Knock-in Reporter Mouse","volume":"11","author":"Comai","year":"2021","journal-title":"Skelet. Muscle"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.1242\/jeb.00635","article-title":"Analysis of myostatin gene structure, expression and function in zebrafish","volume":"206","author":"Xu","year":"2003","journal-title":"J. Exp. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1249\/JES.0000000000000012","article-title":"Fiber Typing in Aging Muscle","volume":"42","author":"Sgarioto","year":"2014","journal-title":"Exerc. Sport Sci. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/embor.2008.73","article-title":"Prdm1- and Sox6-Mediated Transcriptional Repression Specifies Muscle Fibre Type in the Zebrafish Embryo","volume":"9","author":"Elworthy","year":"2008","journal-title":"EMBO. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Bellanti, F., Buglio, A.L., and Vendemiale, G. (2021). Mitochondrial Impairment in Sarcopenia. Biology, 10.","DOI":"10.3390\/biology10010031"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Acuna-Castroviejo, D. (2007). Melatonin Role in the Mitochondrial Function. Front. Biosci., 12.","DOI":"10.2741\/2116"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ichii, S., Matsuoka, I., Okazaki, F., and Shimada, Y. (2022). Zebrafish Models for Skeletal Muscle Senescence: Lessons from Cell Cultures and Rodent Models. Molecules, 27.","DOI":"10.3390\/molecules27238625"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"331","DOI":"10.2144\/000112909","article-title":"Real-Time Imaging of Mitochondria in Transgenic Zebrafish Expressing Mitochondrially Targeted GFP","volume":"45","author":"Kim","year":"2008","journal-title":"Biotechniques"},{"key":"ref_48","unstructured":"Westerfield, M. (2007). The Zebrafish Book, A Guide for the Laboratory Use of Zebrafish (Danio Rerio), Univ Oregon Press."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/11\/6166\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:53:14Z","timestamp":1760107994000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/11\/6166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,3]]},"references-count":48,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["ijms25116166"],"URL":"https:\/\/doi.org\/10.3390\/ijms25116166","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,3]]}}}