{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:26:27Z","timestamp":1791390387565,"version":"4.3.3"},"reference-count":39,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T00:00:00Z","timestamp":1718150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["JP24659357"],"award-info":[{"award-number":["JP24659357"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>It has been reported that Mizoribine is an immunosuppressant used to suppress rejection in renal transplantation, nephrotic syndrome, lupus nephritis, and rheumatoid arthritis. The molecular chaperone HSP60 alone induces inflammatory cytokine IL-6 and the co-chaperone HSP10 alone inhibits IL-6 induction. HSP60 and HSP10 form a complex in the presence of ATP. We analyzed the effects of Mizoribine, which is structurally similar to ATP, on the structure and physiological functions of HSP60\u2013HSP10 using Native\/PAGE and transmission electron microscopy. At low concentrations of Mizoribine, no complex formation of HSP60\u2013HSP10 was observed, nor was the expression of IL-6 affected. On the other hand, high concentrations of Mizoribine promoted HSP60\u2013HSP10 complex formation and consequently suppressed IL-6 expression. Here, we propose a novel mechanism of immunosuppressive action of Mizoribine.<\/jats:p>","DOI":"10.3390\/ijms25126452","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T04:15:27Z","timestamp":1718165727000},"page":"6452","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mizoribine Promotes Molecular Chaperone HSP60\/HSP10 Complex Formation"],"prefix":"10.3390","volume":"25","author":[{"given":"Atsuko","family":"Miura","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Akita University Graduate School of Medicine, Akita 010-8543, Japan"},{"name":"Department of Life Science, Akita Cerebrospinal and Cardiovascular Center, Akita 010-0874, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiko","family":"Narita","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Akita University Graduate School of Medicine, Akita 010-8543, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9610-7464","authenticated-orcid":false,"given":"Taku","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Department of Life Science, Akita Cerebrospinal and Cardiovascular Center, Akita 010-0874, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5738-1332","authenticated-orcid":false,"given":"Hiroaki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Akita University Graduate School of Medicine, Akita 010-8543, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1007\/BF01922237","article-title":"Anti-arthritic activity of bredinin, an immunosuppressive agent","volume":"33","author":"Iwata","year":"1977","journal-title":"Experientia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1016\/0006-2952(83)90301-5","article-title":"Genetic and biochemical studies on the activation and cytotoxic mechanism of bredinin, a potent inhibitor of purine biosynthesis in mammalian cells","volume":"32","author":"Koyama","year":"1983","journal-title":"Biochem. 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