{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T23:53:37Z","timestamp":1785282817047,"version":"3.55.0"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D program of China","award":["2023YFD1801100"],"award-info":[{"award-number":["2023YFD1801100"]}]},{"name":"National Key R&amp;D program of China","award":["31873034"],"award-info":[{"award-number":["31873034"]}]},{"name":"National Key R&amp;D program of China","award":["88658300"],"award-info":[{"award-number":["88658300"]}]},{"name":"National Key R&amp;D program of China","award":["54970612"],"award-info":[{"award-number":["54970612"]}]},{"name":"National Natural Science Foundation of China","award":["2023YFD1801100"],"award-info":[{"award-number":["2023YFD1801100"]}]},{"name":"National Natural Science Foundation of China","award":["31873034"],"award-info":[{"award-number":["31873034"]}]},{"name":"National Natural Science Foundation of China","award":["88658300"],"award-info":[{"award-number":["88658300"]}]},{"name":"National Natural Science Foundation of China","award":["54970612"],"award-info":[{"award-number":["54970612"]}]},{"name":"Beijing Dairy Industry Innovation Team, the program for Postdoctoral of Heilongjiang Province","award":["2023YFD1801100"],"award-info":[{"award-number":["2023YFD1801100"]}]},{"name":"Beijing Dairy Industry Innovation Team, the program for Postdoctoral of Heilongjiang Province","award":["31873034"],"award-info":[{"award-number":["31873034"]}]},{"name":"Beijing Dairy Industry Innovation Team, the program for Postdoctoral of Heilongjiang Province","award":["88658300"],"award-info":[{"award-number":["88658300"]}]},{"name":"Beijing Dairy Industry Innovation Team, the program for Postdoctoral of Heilongjiang Province","award":["54970612"],"award-info":[{"award-number":["54970612"]}]},{"name":"Northeast Agricultural University Young Talents","award":["2023YFD1801100"],"award-info":[{"award-number":["2023YFD1801100"]}]},{"name":"Northeast Agricultural University Young Talents","award":["31873034"],"award-info":[{"award-number":["31873034"]}]},{"name":"Northeast Agricultural University Young Talents","award":["88658300"],"award-info":[{"award-number":["88658300"]}]},{"name":"Northeast Agricultural University Young Talents","award":["54970612"],"award-info":[{"award-number":["54970612"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Trueperella pyogenes is an important opportunistic pathogenic bacterium widely distributed in the environment. Pyolysin (PLO) is a primary virulence factor of T. pyogenes and capable of lysing many different cells. PLO is a member of the cholesterol-dependent cytolysin (CDC) family of which the primary structure only presents a low level of homology with other members from 31% to 45%. By deeply studying PLO, we can understand the overall pathogenic mechanism of CDC family proteins. This study established a mouse muscle tissue model infected with recombinant PLO (rPLO) and its single-point mutations, rPLO N139K and rPLO F240A, and explored its mechanism of causing inflammatory damage. The inflammatory injury abilities of rPLO N139K and rPLO F240A are significantly reduced compared to rPLO. This study elaborated on the inflammatory mechanism of PLO by examining its unit point mutations in detail. Our data also provide a theoretical basis and practical significance for future research on toxins and bacteria.<\/jats:p>","DOI":"10.3390\/ijms25126703","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T03:46:29Z","timestamp":1718855189000},"page":"6703","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Revealing the Mechanism of NLRP3 Inflammatory Pathway Activation through K+ Efflux Induced by PLO via Signal Point Mutations"],"prefix":"10.3390","volume":"25","author":[{"given":"Qiang","family":"Shan","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"},{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bolin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0183-5611","authenticated-orcid":false,"given":"Jiufeng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaohong","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"},{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100093, China"},{"name":"Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, M.L., Liu, M.C., Xu, J., An, L.G., Wang, J.F., and Zhu, Y.H. 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