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(IMII)","award":["152220002"],"award-info":[{"award-number":["152220002"]}]},{"name":"Millenium Institute in Immunology and Immunotherapy, Santiago, Chile (IMII)","award":["03-2022"],"award-info":[{"award-number":["03-2022"]}]},{"name":"Millenium Institute in Immunology and Immunotherapy, Santiago, Chile (IMII)","award":["ICN09_016\/ICN2021_45"],"award-info":[{"award-number":["ICN09_016\/ICN2021_45"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Long non-coding RNAs (lncRNAs) are nucleotide sequences that participate in different biological processes and are associated with different pathologies, including cancer. Long intergenic non-protein-coding RNA 662 (LINC00662) has been reported to be involved in different cancers, including colorectal, prostate, and breast cancer. However, its role in gallbladder cancer has not yet been described. In this article, we hypothesize that LINC00662 has an important role in the acquisition of aggressiveness traits such as a stem-like phenotype, invasion, and chemoresistance in gallbladder cancer. Here, we show that LINC00662 is associated with larger tumor size and lymph node metastasis in patients with gallbladder cancer. Furthermore, we show that the overexpression of LINC00662 promotes an increase in CD133+\/CD44+ cell populations and the expression of stemness-associated genes. LINC00662 promotes greater invasive capacity and the expression of genes associated with epithelial\u2013mesenchymal transition. In addition, the expression of LINC00662 promotes resistance to cisplatin and 5-fluorouracil, associated with increased expression of chemoresistance-related ATP-binding cassette (ABC) transporters in gallbladder cancer (GBC) cell lines. Finally, we show that the mechanism by which LINC00662 exerts its function is through a decrease in microRNA 335-5p (miR-335-5p) and an increase in octamer-binding transcription factor 4 (OCT4) in GBC cells. Thus, our data allow us to propose LINC00662 as a biomarker of poor prognosis and a potential therapeutic target for patients with GBC.<\/jats:p>","DOI":"10.3390\/ijms25126740","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T03:46:29Z","timestamp":1718855189000},"page":"6740","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["LINC00662 Promotes Aggressive Traits by Modulating OCT4 Expression through miR-335-5p in Gallbladder Cancer Cells"],"prefix":"10.3390","volume":"25","author":[{"given":"Pablo","family":"P\u00e9rez-Moreno","sequence":"first","affiliation":[{"name":"Programa de Comunicaci\u00f3n Celular en C\u00e1ncer, Facultad de Medicina Cl\u00ednica Alemana, Universidad del Desarrollo, Santiago 7780272, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9545-3971","authenticated-orcid":false,"given":"Ismael","family":"Riquelme","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Faculty of Health Sciences, Universidad Aut\u00f3noma de Chile, Temuco 4810101, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Bizama","sequence":"additional","affiliation":[{"name":"Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevenci\u00f3n y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Vergara-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Translational Medicine (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Biomedicine and Translational Research Lab, Universidad de la Frontera, Temuco 4810296, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1678-2708","authenticated-orcid":false,"given":"Julio C.","family":"Tapia","sequence":"additional","affiliation":[{"name":"Programa de Biolog\u00eda Celular y Molecular, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscilla","family":"Brebi","sequence":"additional","affiliation":[{"name":"Laboratory of Integrative Biology (LIBi), Millennium Institute on Immunology and Immunotherapy (MIII), Center for Excellence in Translational Medicine\u2014Scientific and Technological Bioresource Nucleus (CEMT-BIOREN), Universidad de La Frontera, Temuco 4810296, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3817-4896","authenticated-orcid":false,"given":"Patricia","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevenci\u00f3n y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Roa","sequence":"additional","affiliation":[{"name":"Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevenci\u00f3n y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","first-page":"99","article-title":"Gallbladder cancer: Epidemiology and outcome","volume":"6","author":"Hundal","year":"2014","journal-title":"Clin. Epidemiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/j.cell.2019.10.017","article-title":"The Role of Non-coding RNAs in Oncology","volume":"179","author":"Slack","year":"2019","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.molcel.2011.08.018","article-title":"Molecular mechanisms of long noncoding RNAs","volume":"43","author":"Wang","year":"2011","journal-title":"Mol. Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"598817","DOI":"10.3389\/fonc.2020.598817","article-title":"Long Non-Coding RNAs: The Regulatory Mechanisms, Research Strategies, and Future Directions in Cancers","volume":"10","author":"Gao","year":"2020","journal-title":"Front. 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Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.canlet.2016.06.019","article-title":"Long non-coding RNA MINCR promotes gallbladder cancer progression through stimulating EZH2 expression","volume":"380","author":"Wang","year":"2016","journal-title":"Cancer Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e1583","DOI":"10.1038\/cddis.2014.541","article-title":"Long non-coding RNA CCAT1 promotes gallbladder cancer development via negative modulation of miRNA-218-5p","volume":"6","author":"Ma","year":"2015","journal-title":"Cell Death Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.gene.2018.12.086","article-title":"Long non-coding RNA DILC promotes the progression of gallbladder carcinoma","volume":"694","author":"Liang","year":"2019","journal-title":"Gene"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"He, Y., Xu, Y., Yu, X., Sun, Z., and Guo, W. (2021). The Vital Roles of LINC00662 in Human Cancers. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.711352"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nrc3184","article-title":"Cancer stem cells: An evolving concept","volume":"12","author":"Nguyen","year":"2012","journal-title":"Nat. Rev. Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.4252\/wjsc.v7.i9.1150","article-title":"Common stemness regulators of embryonic and cancer stem cells","volume":"7","author":"Hadjimichael","year":"2015","journal-title":"World J. Stem Cells"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Begicevic, R.R., and Falasca, M. (2017). ABC Transporters in Cancer Stem Cells: Beyond Chemoresistance. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112362"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1172\/JCI39104","article-title":"The basics of epithelial-mesenchymal transition","volume":"119","author":"Kalluri","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3335","DOI":"10.2147\/OTT.S305098","article-title":"Functions and Targets of miR-335 in Cancer","volume":"14","author":"Ye","year":"2021","journal-title":"Onco Targets Ther."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s12943-019-0947-9","article-title":"Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143\/HK2 axis in gallbladder cancer","volume":"18","author":"Chen","year":"2019","journal-title":"Mol. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.ncrna.2022.02.004","article-title":"Long noncoding RNAs as promising biomarkers in cancer","volume":"7","author":"Beylerli","year":"2022","journal-title":"Noncoding RNA Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1139\/bcb-2019-0396","article-title":"Silencing linc00662 inhibits cell proliferation and colony formation of lung cancer cells via regulating the miR-145-5p-PAFAH1B2 axis","volume":"99","author":"Xu","year":"2021","journal-title":"Biochem. Cell Biol."},{"key":"ref_19","first-page":"3688","article-title":"Long noncoding RNA LINC00662 functions as miRNA sponge to promote the prostate cancer tumorigenesis through targeting miR-34a","volume":"23","author":"Li","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_20","first-page":"229","article-title":"Long non-coding RNA LINC00662 promotes proliferation and migration of breast cancer cells via regulating the miR-497-5p\/EglN2 axis","volume":"67","author":"Cheng","year":"2020","journal-title":"Acta Biochim. Pol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Moreno, P., Riquelme, I., Garc\u00eda, P., Brebi, P., and Roa, J.C. (2022). Environmental and Lifestyle Risk Factors in the Carcinogenesis of Gallbladder Cancer. J. Pers. Med., 12.","DOI":"10.3390\/jpm12020234"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/nm.4409","article-title":"Cancer stem cells revisited","volume":"23","author":"Batlle","year":"2017","journal-title":"Nat. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1056\/NEJMra061808","article-title":"Cancer stem cells","volume":"355","author":"Jordan","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/s12935-022-02576-0","article-title":"LINC00662 enhances cell progression and stemness in breast cancer by MiR-144-3p\/SOX2 axis","volume":"22","author":"An","year":"2022","journal-title":"Cancer Cell Int."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nunes, T., Hamdan, D., Leboeuf, C., El Bouchtaoui, M., Gapihan, G., Nguyen, T.T., Meles, S., Angeli, E., Ratajczak, P., and Lu, H. (2018). Targeting Cancer Stem Cells to Overcome Chemoresistance. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19124036"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/s41586-019-1730-1","article-title":"A view on drug resistance in cancer","volume":"575","author":"Vasan","year":"2019","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.canlet.2009.05.020","article-title":"Side population and cancer stem cells: Therapeutic implications","volume":"288","author":"Moserle","year":"2010","journal-title":"Cancer Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"14228","DOI":"10.1073\/pnas.0400067101","article-title":"A distinct \u201cside population\u201d of cells with high drug efflux capacity in human tumor cells","volume":"101","author":"Foster","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4336","DOI":"10.1111\/febs.16046","article-title":"The gate to metastasis: Key players in cancer cell intravasation","volume":"289","author":"Sznurkowska","year":"2022","journal-title":"FEBS J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/jb\/mvy078","article-title":"Long non-coding RNA LINC00662 promotes cell invasion and contributes to cancer stem cell-like phenotypes in lung cancer cells","volume":"164","author":"Gong","year":"2018","journal-title":"J. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"640","DOI":"10.3349\/ymj.2021.62.7.640","article-title":"LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p\/EZH2 Axis","volume":"62","author":"Yao","year":"2021","journal-title":"Yonsei Med. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.semcancer.2023.10.001","article-title":"Transcriptional regulation of EMT transcription factors in cancer","volume":"97","author":"Saitoh","year":"2023","journal-title":"Semin. Cancer Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3389\/fonc.2018.00381","article-title":"EMT and Stemness in Tumor Dormancy and Outgrowth: Are They Intertwined Processes?","volume":"8","author":"Weidenfeld","year":"2018","journal-title":"Front. Oncol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8381","DOI":"10.1002\/jcp.27740","article-title":"Cancer stem cells (CSCs) in cancer progression and therapy","volume":"234","author":"Najafi","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1038\/s41388-018-0432-8","article-title":"Long noncoding RNA PVT1-214 promotes proliferation and invasion of colorectal cancer by stabilizing Lin28 and interacting with miR-128","volume":"38","author":"He","year":"2019","journal-title":"Oncogene"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.2147\/OTT.S191740","article-title":"lncRNA SLCO4A1-AS1 promotes growth and invasion of bladder cancer through sponging miR-335-5p to upregulate OCT4","volume":"12","author":"Yang","year":"2019","journal-title":"Onco Targets Ther."},{"key":"ref_37","first-page":"1853","article-title":"MiR-335-5p inhibits proliferation of Huh-7 liver cancer cells via targeting the OCT4\/Akt pathway","volume":"25","author":"Ji","year":"2021","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s12935-017-0398-6","article-title":"miR-335 negatively regulates osteosarcoma stem cell-like properties by targeting POU5F1","volume":"17","author":"Guo","year":"2017","journal-title":"Cancer Cell Int."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8309","DOI":"10.1007\/s13277-014-2092-9","article-title":"MiR-335 functions as a tumor suppressor in pancreatic cancer by targeting OCT4","volume":"35","author":"Gao","year":"2014","journal-title":"Tumour Biol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhang, X., Wang, W., Zhu, W., Dong, J., Cheng, Y., Yin, Z., and Shen, F. (2019). Mechanisms and Functions of Long Non-Coding RNAs at Multiple Regulatory Levels. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20225573"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1093\/nar\/gks596","article-title":"Primer3\u2013new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/12\/6740\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:01:02Z","timestamp":1760108462000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/12\/6740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,19]]},"references-count":41,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["ijms25126740"],"URL":"https:\/\/doi.org\/10.3390\/ijms25126740","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,19]]}}}