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Neat PLA and PLA\/PMMA hybrid scaffolds were developed successfully through a (CF\/DMF) solvent system, obtaining a random fiber deposition that generated a porous structure with pore interconnectivity. However, with the solvent system used, it was not possible to generate fibers in the case of the neat PMMA sample. With the increase in the amount of PMMA in PLA\/PMMA ratios, the fiber diameter of hybrid scaffolds decreases, and the defects (beads) in the fiber structure increase; these beads are associated with a nanoparticle agglomeration, that could be related to a low interaction between n-BG and the polymer matrix. The Young\u2019s modulus of PLA\/PMMA\/n-BG decreases by 34 and 80%, indicating more flexible behavior compared to neat PLA. The PLA\/PMMA\/n-BG scaffolds showed a bioactive property related to the presence of hydroxyapatite crystals in the fiber surface after 28 days of immersion in a Simulated Body Fluids solution (SBF). In addition, the hydrolytic degradation process of PLA\/PMMA\/n-BG, analyzed after 35 days of immersion in a phosphate-buffered saline solution (PBS), was less than that of the pure PLA. The in vitro analysis using an HBOF-1.19 cell line indicated that the PLA\/PMMA\/n-BG scaffold showed good cell viability and was able to promote cell proliferation after 7 days. On the other hand, the in vivo biocompatibility evaluated via a subdermal model in BALC male mice corroborated the good behavior of the scaffolds in avoiding the generation of a cytotoxic effect and being able to enhance the healing process, suggesting that the materials are suitable for potential applications in tissue engineering.<\/jats:p>","DOI":"10.3390\/ijms25136843","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T11:10:28Z","timestamp":1718968228000},"page":"6843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development of Bioactive Hybrid Poly(lactic acid)\/Poly(methyl methacrylate) (PLA\/PMMA) Electrospun Fibers Functionalized with Bioglass Nanoparticles for Bone Tissue Engineering Applications"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2506-4623","authenticated-orcid":false,"given":"Fabi\u00e1n","family":"\u00c1lvarez-Carrasco","sequence":"first","affiliation":[{"name":"Laboratorio de Biomec\u00e1nica y Biomateriales, Departamento de Ingenier\u00eda Mec\u00e1nica, Facultad de Ingenier\u00eda, Universidad de Santiago de Chile, Santiago 9160000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Varela","sequence":"additional","affiliation":[{"name":"Laboratorio de Biomec\u00e1nica y Biomateriales, Departamento de Ingenier\u00eda Mec\u00e1nica, Facultad de Ingenier\u00eda, Universidad de Santiago de Chile, Santiago 9160000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio A.","family":"Sarabia-Vallejos","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda, Arquitectura y Dise\u00f1o, Universidad San Sebasti\u00e1n, Santiago 8420524, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1283-8551","authenticated-orcid":false,"given":"Claudio","family":"Garc\u00eda-Herrera","sequence":"additional","affiliation":[{"name":"Laboratorio de Biomec\u00e1nica y Biomateriales, Departamento de Ingenier\u00eda Mec\u00e1nica, Facultad de Ingenier\u00eda, Universidad de Santiago de Chile, Santiago 9160000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Grupo Pol\u00edmeros, Departamento de Ciencias del Ambiente, Facultad de Qu\u00edmica y Biolog\u00eda, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago 9160000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3361-6226","authenticated-orcid":false,"given":"Paula A.","family":"Zapata","sequence":"additional","affiliation":[{"name":"Grupo Pol\u00edmeros, Departamento de Ciencias del Ambiente, Facultad de Qu\u00edmica y Biolog\u00eda, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago 9160000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0852-8948","authenticated-orcid":false,"given":"Diana","family":"Z\u00e1rate-Trivi\u00f1o","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda y Virolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Jos\u00e9","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Centro de Ingenier\u00eda y Desarrollo Industrial, Av. 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Manuf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s40204-019-00125-z","article-title":"Bone tissue regeneration: Biology, strategies and interface studies","volume":"8","author":"Ansari","year":"2019","journal-title":"Prog. Biomater."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Battafarano, G., Rossi, M., De Martino, V., Marampon, F., Borro, L., Secinaro, A., and Del Fattore, A. (2021). Strategies for bone regeneration: From graft to tissue engineering. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031128"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chocholata, P., Kulda, V., and Babuska, V. (2019). Fabrication of scaffolds for bone-tissue regeneration. Materials, 12.","DOI":"10.3390\/ma12040568"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1515\/biolog-2016-0056","article-title":"Scaffolds from biomaterials: Advantages and limitations in bone and tissue engineering","volume":"71","author":"Alaribe","year":"2016","journal-title":"Biologia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1039\/C4BM00291A","article-title":"Bone tissue regeneration: The role of scaffold geometry","volume":"3","author":"Zadpoor","year":"2014","journal-title":"Biomater. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1093\/rb\/rby001","article-title":"Effect of microporosity on scaffolds for bone tissue engineering","volume":"5","author":"Zhang","year":"2018","journal-title":"Regen. Biomater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/2041731412443530","article-title":"A short review: Recent advances in electrospinning for bone tissue regeneration","volume":"3","author":"Shin","year":"2012","journal-title":"J. Tissue Eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1515\/epoly-2020-0068","article-title":"Structural design toward functional materials by electrospinning: A review","volume":"20","author":"Yang","year":"2020","journal-title":"e-Polymers"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1080\/15583724.2019.1688830","article-title":"Fabrication of porous fibers via electrospinning: Strategies and applications","volume":"60","author":"Huang","year":"2020","journal-title":"Polym. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.progpolymsci.2014.12.001","article-title":"Electrospinning of polymer nanofibers for tissue regeneration","volume":"46","author":"Jiang","year":"2015","journal-title":"Prog. Polym. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.eurpolymj.2017.12.025","article-title":"Fabricating porous poly(lactic acid) fibres via electrospinning","volume":"99","author":"Huang","year":"2018","journal-title":"Eur. Polym. J."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Peranidze, K., Safronova, T.V., and Kildeeva, N.R. (2022). Fibrous polymer-based composites obtained by electrospinning for bone tissue engineering. Polymers, 14.","DOI":"10.3390\/polym14010096"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1080\/00914037.2021.1944140","article-title":"Poly lactic acid (PLA) polymers: From properties to biomedical applications","volume":"71","author":"Ebrahimi","year":"2021","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.biotechadv.2011.06.019","article-title":"Poly-lactic acid synthesis for application in biomedical devices\u2014A review","volume":"30","author":"Lasprilla","year":"2012","journal-title":"Biotechnol. Adv."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.ijbiomac.2022.05.047","article-title":"Electrospun fibers of poly (lactic acid) containing bioactive glass and magnesium oxide nanoparticles for bone tissue regeneration","volume":"210","author":"Canales","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","first-page":"213578","article-title":"Preparation and characterization of novel poly (lactic acid)\/calcium oxide nanocomposites by electrospinning as a potential bone tissue scaffold","volume":"153","author":"Canales","year":"2023","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"19935","DOI":"10.1038\/s41598-022-24275-6","article-title":"Melt electrowriting of PLA, PCL, and composite PLA\/PCL scaffolds for tissue engineering application","volume":"12","author":"Shahverdi","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9246802","DOI":"10.1155\/2017\/9246802","article-title":"PLA-Based Hybrid and Composite Electrospun Fibrous Scaffolds as Potential Materials for Tissue Engineering","volume":"2017","author":"Magiera","year":"2017","journal-title":"J. Nanomater."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lv, S., Zhao, X., Shi, L., Zhang, G., Wang, S., Kang, W., and Zhuang, X. (2018). Preparation and properties of sc-PLA\/PMMA transparent nanofiber air filter. Polymers, 10.","DOI":"10.3390\/polym10090996"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1080\/15583724.2015.1031377","article-title":"A Review of the Properties and Applications of Poly (Methyl Methacrylate) (PMMA)","volume":"55","author":"Ali","year":"2015","journal-title":"Polym. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"110378","DOI":"10.1016\/j.msec.2019.110378","article-title":"Polymethyl methacrylate (PMMA) grafted collagen scaffold reinforced by PdO\u2013TiO2 nanocomposites","volume":"108","author":"Vedhanayagam","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ramanathan, S., Lin, Y.-C., Thirumurugan, S., Hu, C.-C., Duann, Y.-F., and Chung, R.-J. (2024). Poly(methyl methacrylate) in Orthopedics: Strategies, Challenges, and Prospects in Bone Tissue Engineering. Polymers, 16.","DOI":"10.3390\/polym16030367"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"24677","DOI":"10.1002\/app.42677","article-title":"Melt blending of polylactide and poly(methyl methacrylate): Thermal and mechanical properties and phase morphology characterization","volume":"132","author":"Anakabe","year":"2015","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"015009","DOI":"10.1088\/1468-6996\/14\/1\/015009","article-title":"In vitro and in vivo evaluation of electrospun PCL\/PMMA fibrous scaffolds for bone regeneration","volume":"14","author":"Son","year":"2013","journal-title":"Sci. Technol. Adv. Mater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.actbio.2019.11.024","article-title":"Bioactive materials: In vitro investigation of different mechanisms of hydroxyapatite precipitation","volume":"102","author":"Ferraris","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/0272-8842(95)00126-3","article-title":"Bioactive materials","volume":"22","author":"Cao","year":"1996","journal-title":"Ceram. Int."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sch\u00e4tzlein, E., Kicker, C., S\u00f6hling, N., Ritz, U., Neijhoft, J., Henrich, D., Frank, J., Marzi, I., and Blaeser, A. (2022). 3D-Printed PLA-Bioglass Scaffolds with Controllable Calcium Release and MSC Adhesion for Bone Tissue Engineering. Polymers, 14.","DOI":"10.3390\/polym14122389"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1002\/jctb.2283","article-title":"Fabrication and characterization of electrospun poly-DL-lactide (PDLLA) fibrous coatings on 45S5 Bioglass\u00ae substrates for bone tissue engineering applications","volume":"85","author":"Yunos","year":"2010","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1002\/jbm.a.36963","article-title":"Effect of bioglass nanoparticles on the properties and bioactivity of poly(lactic acid) films","volume":"108","author":"Canales","year":"2020","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.ijbiomac.2022.12.195","article-title":"Fabrication and assessment of bifunctional electrospun poly(l-lactic acid) scaffolds with bioglass and zinc oxide nanoparticles for bone tissue engineering","volume":"228","author":"Canales","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.msec.2016.09.008","article-title":"A comprehensive study on the fabrication and properties of biocomposites of poly(lactic acid)\/ceramics for bone tissue engineering","volume":"70","author":"Tajbakhsh","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.matlet.2010.01.014","article-title":"Composite nanofiber of bioactive glass nanofiller incorporated poly(lactic acid) for bone regeneration","volume":"64","author":"Noh","year":"2010","journal-title":"Mater. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1900287","DOI":"10.1002\/adem.201900287","article-title":"Bioact\u0131ve Glass-Polymer Nanocomposites for Bone T\u0131ssue Regeneration Applicat\u0131ons: A Rev\u0131ew","volume":"21","author":"Unalan","year":"2019","journal-title":"Adv. Eng. Mater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12716","DOI":"10.1021\/acsomega.0c00180","article-title":"Bioactive Glass Nanoparticles for Tissue Regeneration","volume":"5","author":"Chatzistavrou","year":"2020","journal-title":"ACS Omega"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Serio, F., Miola, M., Vern\u00e8, E., Pisignano, D., Boccaccini, A.R., and Liverani, L. (2019). Electrospun filaments embedding bioactive glass particles with ion release and enhanced mineralization. Nanomaterials, 9.","DOI":"10.3390\/nano9020182"},{"key":"ref_37","first-page":"55","article-title":"Incorporation of bioactive glass nanoparticles in electrospun PCL\/chitosan fibers by using benign solvents","volume":"3","author":"Liverani","year":"2018","journal-title":"Bioact. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s12221-015-0245-0","article-title":"Enhanced bioactivity of osteoblast-like cells on poly(lactic acid)\/poly(methyl methacrylate)\/nano-hydroxyapatite scaffolds for bone tissue engineering","volume":"16","author":"Rong","year":"2015","journal-title":"Fibers Polym."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"494014","DOI":"10.1088\/0957-4484\/22\/49\/494014","article-title":"Preparation and characterization of bioactive glass nanoparticles prepared by sol\u2013gel for biomedical applications","volume":"22","author":"Luz","year":"2011","journal-title":"Nanotechnology"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Duta, L., Popescu, A., Zgura, I., Preda, N., and Mihailescu, I. (2015). Wettability of Nanostructured Surfaces. Wetting and Wettability, InTech.","DOI":"10.5772\/60808"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"100075","DOI":"10.1016\/j.mtsust.2021.100075","article-title":"Improvement of hydrophilicity and cell attachment of polycaprolactone scaffolds using green synthesized carbon dots","volume":"13","author":"Ehtesabi","year":"2021","journal-title":"Mater. Today Sustain."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"101140","DOI":"10.1016\/j.surfin.2021.101140","article-title":"Electrospun Nanofibers as Effective Superhydrophobic Surfaces: A Brief review","volume":"24","author":"Raman","year":"2021","journal-title":"Surf. Interfaces"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5583","DOI":"10.1016\/j.apsusc.2005.12.142","article-title":"PMMA nanofibers production by electrospinning","volume":"252","author":"Piperno","year":"2006","journal-title":"Appl. Surf. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Liao, G.Y., Zhou, X.P., Xie, X.L., and Mai, Y.W. (2016). Electrospun polymer scaffolds: Their biomedical and mechanical properties. Springer Series in Biomaterials Science and Engineering, Springer Science and Business Media, LLC.","DOI":"10.1007\/978-3-662-53574-5_8"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2100153","DOI":"10.1002\/adem.202100153","article-title":"Mechanical Properties of Electrospun Fibers\u2014A Critical Review","volume":"23","author":"Rashid","year":"2021","journal-title":"Adv. Eng. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Salimbeigi, G., Cahill, P., and McGuinness, G. (2022). Solvent system effects on the physical and mechanical properties of electrospun Poly(\u03b5-caprolactone) scaffolds for in vitro lung models. J. Mech. Behav. Biomed. Mater., 136.","DOI":"10.1016\/j.jmbbm.2022.105493"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"107119","DOI":"10.1016\/j.polymertesting.2021.107119","article-title":"Effect of electrospinning parameters on the mechanical and morphological characteristics of small diameter PCL tissue engineered blood vessel scaffolds having distinct micro and nano fibre populations\u2014A DOE approach","volume":"96","author":"Cahill","year":"2021","journal-title":"Polym. Test."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Dolgin, J., Hanumantharao, S.N., Farias, S., Simon, C.G., and Rao, S. (2023). Mechanical properties and morphological alterations in fiber-based scaffolds affecting tissue engineering outcomes. Fibers, 11.","DOI":"10.3390\/fib11050039"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"105773","DOI":"10.1016\/j.mtcomm.2023.105773","article-title":"The effect of molecular weight and fiber diameter on the mechanical properties of single, electrospun PCL nanofibers","volume":"35","author":"Alharbi","year":"2023","journal-title":"Mater. Today Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4080","DOI":"10.3390\/ma8074080","article-title":"Fabrication and characterization of electrospun PCL-MgO-keratin-based composite nanofibers for biomedical applications","volume":"8","author":"Boakye","year":"2015","journal-title":"Materials"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Luginina, M., Schuhladen, K., Orr\u00fa, R., Cao, G., Boccaccini, A.R., and Liverani, L. (2020). Electrospun PCL\/PGS composite fibers incorporating bioactive glass particles for soft tissue engineering applications. Nanomaterials, 10.","DOI":"10.3390\/nano10050978"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.matchemphys.2009.08.011","article-title":"Electrospun nanofibrous biodegradable polyester coatings on Bioglass\u00ae-based glass-ceramics for tissue engineering","volume":"118","author":"Bretcanu","year":"2009","journal-title":"Mater. Chem. Phys."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Filip, D.G., Surdu, V.-A., Paduraru, A.V., and Andronescu, E. (2022). Current Development in Biomaterials\u2014Hydroxyapatite and Bioglass for Applications in Biomedical Field: A Review. J. Funct. Biomater., 13.","DOI":"10.3390\/jfb13040248"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Bi, H., Feng, T., Li, B., and Han, Y. (2020). In vitro and in vivo comparison study of electrospun pla and pla\/pva\/sa fiber membranes for wound healing. Polymers, 12.","DOI":"10.3390\/polym12040839"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1002\/jbm.b.34606","article-title":"Mechanical and degradation properties of poly(methyl methacrylate) cement\/borate bioactive glass composites","volume":"108","author":"Cole","year":"2020","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Chelu, M., and Musuc, A.M. (2023). Advanced Biomedical Applications of Multifunctional Natural and Synthetic Biomaterials. Processes, 11.","DOI":"10.3390\/pr11092696"},{"key":"ref_57","first-page":"117","article-title":"Evaluation of biocompatibility using in vitro methods: Interpretation and limitations","volume":"126","author":"Bruinink","year":"2012","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1016\/j.eurpolymj.2008.01.009","article-title":"Supercritical CO2 antisolvent precipitation of polymer networks of l-PLA, PMMA and PMMA\/PCL blends for biomedical applications","volume":"44","author":"Domingo","year":"2008","journal-title":"Eur. Polym. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1163\/156856212X623526","article-title":"Enhanced osteoblast responses to poly(methyl methacrylate)\/hydroxyapatite electrospun nanocomposites for bone tissue engineering","volume":"24","author":"Xing","year":"2013","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zaszczy\u0144ska, A., Ko\u0142buk, D., Gradys, A., and Sajkiewicz, P. (2024). Development of Poly (methyl methacrylate)\/nano-hydroxyapatite (PMMA\/nHA) Nanofibers for Tissue Engineering Regeneration Using an Electrospinning Technique. Polymers, 16.","DOI":"10.3390\/polym16040531"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1021\/acsbiomaterials.8b00252","article-title":"The Foreign Body Response Demystified","volume":"5","author":"Chandorkar","year":"2019","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"20161057","DOI":"10.1098\/rsif.2016.1057","article-title":"Enhanced osteointegration of poly(methylmethacrylate) bone cements by incorporating strontium-containing borate bioactive glass","volume":"14","author":"Cui","year":"2017","journal-title":"J. R. Soc. Interface"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Sharifi, E., Sadati, S.A., Yousefiasl, S., Sartorius, R., Zafari, M., Rezakhani, L., Alizadeh, M., Zare, E.N., Omidghaemi, S., and Ghanavatinejad, F. (2022). Cell loaded hydrogel containing Ag-doped bioactive glass\u2013ceramic nanoparticles as skin substitute: Antibacterial properties, immune response, and scarless cutaneous wound regeneration. Bioeng. Transl. Med., 7.","DOI":"10.1002\/btm2.10386"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.21873\/invivo.12941","article-title":"In Vivo Analysis of the Immune Response to Strontium- and Copper-doped Bioglass","volume":"36","author":"Barbeck","year":"2022","journal-title":"In Vivo"},{"key":"ref_65","unstructured":"(2018). American Society for Testing and Materials. Standard Test Method for Tensile Properties of Thin Plastic Sheeting (Standard No. D882-12)."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1016\/j.biomaterials.2006.01.017","article-title":"How useful is SBF in predicting in vivo bone bioactivity?","volume":"27","author":"Kokubo","year":"2006","journal-title":"Biomaterials"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1080\/09205063.2015.1088125","article-title":"Preparation of hydrophilic poly(lactic acid) tissue engineering scaffold via (PLA)-(PLA-b-PEG)-(PEG) solution casting and thermal-induced surface structural transformation","volume":"26","author":"Zhu","year":"2015","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_68","unstructured":"(2016). Biological Evaluation of Medical Devices\u2014Part 6: Tests for Local Effects after Implantation (Standard No. ISO 10993-6:2016)."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/6843\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:02:33Z","timestamp":1760108553000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/6843"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,21]]},"references-count":68,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ijms25136843"],"URL":"https:\/\/doi.org\/10.3390\/ijms25136843","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,21]]}}}