{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:49:02Z","timestamp":1787028542903,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T00:00:00Z","timestamp":1719014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Dupuytren\u2019s disease, a chronic and progressive fibroproliferative lesion of the hand, which affects the palmar fascia, has a recurrence rate after selective aponeurotomy of 20\u201340% at 5 years. This study focused, for the first time, on the microanatomical and histopathological characteristics of the longitudinal and vertical fibres (usually spared during surgery) in the aponeurosis with Dupuytren\u2019s disease, in different stages of the Tubiana\u2019s classification. Twelve human samples were collected and analysed by immunostaining, Total Collagen Assay, ELISA Immunoassay, and immunoblotting for the Von Willebrand factor, \u03b1-Sma, D2-40, CD-68, Total Collagen, Collagen-I and III, IL1\u03b2, TNF-\u03b1 to analyse the blood and lymphatic vascularization, the amount and distribution of collagen, and the inflammation. The results show a progressive increase in the arterial vascularization in the vertical fibres (from 8.8\/mm2 in the early stage to 21.4\/mm2 in stage 3\/4), and a parallel progressive decrease in the lymphatic drainage (from 6.2\/mm2 to 2.8\/mm2), correlated with a local inflammatory context (increase in IL-1\u03b2 and TNF-\u03b1 until the stage 2) in both the longitudinal and vertical fibres. The acute inflammation after stage 2 decreased, in favour of a fibrotic action, with the clear synthesis of new collagen (up to ~83 \u00b5g\/mg), especially Collagen-I. These results clearly demonstrate the involvement of the septa of Legueu and Juvara in the disease pathology and the modifications with the disease\u2019s progression. A greater understanding of the pathology becomes fundamental for staging and the adequate therapeutic timing, to obtain the best morpho-functional result and the lowest risk of complications.<\/jats:p>","DOI":"10.3390\/ijms25136865","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T08:42:03Z","timestamp":1719218523000},"page":"6865","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren\u2019s Palmar Aponeurosis and Innovative Clinical Implications"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4372-7889","authenticated-orcid":false,"given":"Caterina","family":"Fede","sequence":"first","affiliation":[{"name":"Department of Neurosciences, Institute of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovico","family":"Coldebella","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, University of Padova, Via Nicol\u00f2 Giustiniani 2, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Petrelli","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, Institute of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Bassetto","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, University of Padova, Via Nicol\u00f2 Giustiniani 2, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesare","family":"Tiengo","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, University of Padova, Via Nicol\u00f2 Giustiniani 2, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-4555","authenticated-orcid":false,"given":"Carla","family":"Stecco","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, Institute of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,22]]},"reference":[{"key":"ref_1","unstructured":"Warwick, D. (2015). Dupuytren\u2019s Disease. FESSH Instructional Course, C. G. Edizioni Medico Scientifiche."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11552-013-9563-0","article-title":"Dupuytren\u2019s disease: Current state of the art","volume":"9","author":"Henry","year":"2014","journal-title":"Hand"},{"key":"ref_3","unstructured":"Tubiana, R., Leclercq, C., Hurst, L.C., Badalamente, M.A., and Mackin, E.J. (2000). Dupuytren\u2019s Disease, Martin Dunitz Ltd."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1302\/2058-5241.4.180021","article-title":"Dupuytren\u2019s disease: Where do we stand?","volume":"4","author":"Grazina","year":"2019","journal-title":"EFORT Open Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s40610-016-0045-3","article-title":"Connective Tissue Degeneration: Mechanisms of Palmar Fascia Degeneration (Dupuytren\u2019s Disease)","volume":"2","author":"Karkampouna","year":"2016","journal-title":"Curr. Mol. Biol. Rep."},{"key":"ref_6","first-page":"443","article-title":"The superficial transverse fibers of the palmar aponeuroses and their significance in Dupuytren\u2019s contracture","volume":"47","author":"Skoog","year":"1967","journal-title":"Surg. Clin. N. Am."},{"key":"ref_7","first-page":"V10140564","article-title":"Behandling af Dupuytrens kontraktur [Dupuytren\u2019s disease treatment]","volume":"177","author":"Dahlin","year":"2015","journal-title":"Ugeskr. Laeger"},{"key":"ref_8","first-page":"781","article-title":"Dupuytren\u2019s Disease-Etiology and Treatment","volume":"118","author":"Ruettermann","year":"2021","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_9","first-page":"648","article-title":"Dupuytren\u2019s Contracture: Current Treatment Methods","volume":"19","author":"Feldman","year":"2017","journal-title":"Isr. Med. Assoc. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s12079-016-0331-0","article-title":"Update on the role of molecular factors and fibroblasts in the pathogenesis of Dupuytren\u2019s disease","volume":"10","author":"Tripoli","year":"2016","journal-title":"J. Cell Commun. Signal"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1002\/ca.23076","article-title":"Hand fasciae innervation: The palmar aponeurosis","volume":"31","author":"Stecco","year":"2018","journal-title":"Clin. Anat."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jhsa.2006.12.011","article-title":"Immunohistochemical evidence of nerve growth factor in Dupuytren\u2019s diseased Palmar Fascia","volume":"32","author":"Lubahn","year":"2007","journal-title":"J. Hand Surg. Am."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.molmed.2022.12.001","article-title":"Dupuytren\u2019s disease: A localised and accessible human fibrotic disorder","volume":"29","author":"Layton","year":"2023","journal-title":"Trends Mol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"787","DOI":"10.2106\/00004623-198163050-00014","article-title":"Biochemical changes in the collagen of the palmar fascia in patients with Dupuytren\u2019s disease","volume":"63","author":"Glimcher","year":"1981","journal-title":"J. Bone Jt. Surg. Am."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"231","DOI":"10.12688\/f1000research.17779.1","article-title":"Recent advances in the understanding of Dupuytren\u2019s disease","volume":"8","author":"Layton","year":"2019","journal-title":"F1000Research"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"130","DOI":"10.5312\/wjo.v9.i9.130","article-title":"Assessment of palmar subcutaneous tissue vascularization in patients with Dupuytren\u2019s contracture","volume":"9","author":"Shchudlo","year":"2018","journal-title":"World J. Orthop."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"420","DOI":"10.3109\/17453674.2013.814011","article-title":"Expression of VEGF, its receptors, and HIF-1\u03b1 in Dupuytren\u2019s disease","volume":"84","author":"Holzer","year":"2013","journal-title":"Acta Orthop."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1002\/jor.25059","article-title":"Circulating inflammatory cytokines alter transcriptional activity within fibrotic tissue of Dupuytren\u2019s disease patients","volume":"40","author":"Wang","year":"2022","journal-title":"J. Orthop. Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Alfonso-Rodr\u00edguez, C.A., Garz\u00f3n, I., Garrido-G\u00f3mez, J., Oliveira, A.C.X., Mart\u00edn-Piedra, M.A., Scionti, G., Carriel, V., Hern\u00e1ndez-Cort\u00e9s, P., Campos, A., and Alaminos, M. (2014). Identification of histological patterns in clinically affected and unaffected palm regions in Dupuytren\u2019s disease. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112457"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.5858\/2000-124-1275-COBABP","article-title":"Changes of biochemical and biomechanical properties in Dupuytren disease","volume":"124","author":"Melling","year":"2000","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1093\/oxfordjournals.jbchem.a124921","article-title":"Ultrastructural and biochemical modifications of collagen from tissue of Morbus Dupuytren patients","volume":"118","author":"Notbohm","year":"1995","journal-title":"J. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.jhsa.2023.06.007","article-title":"Targeting Myofibroblasts as a Treatment Modality for Dupuytren Disease","volume":"48","author":"Ziegler","year":"2023","journal-title":"J. Hand Surg. Am."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1016\/j.jhsa.2006.08.012","article-title":"Factors in the pathogenesis of Dupuytren\u2019s contracture","volume":"31","author":"Qattan","year":"2006","journal-title":"J. Hand Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/978-1-4939-7113-8_19","article-title":"Type I Collagen Purification from Rat Tail Tendons","volume":"1627","year":"2017","journal-title":"Meth. Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Satish, L., Gallo, P.H., Baratz, M.E., Johnson, S., and Kathju, S. (2011). Reversal of TGF-\u03b21 stimulation of \u03b1-smooth muscle actin and extracellular matrix components by cyclic AMP in Dupuytren\u2019s-derived fibroblasts. BMC Musculoskelet. Disord., 12.","DOI":"10.1186\/1471-2474-12-113"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/ase.218","article-title":"Body parts removed during surgery: A useful training source","volume":"4","author":"Macchi","year":"2011","journal-title":"Anat. Sci. Ed."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ab.2019.03.010","article-title":"Ponceau S waste: Ponceau S staining for total protein normalization","volume":"575","author":"Sander","year":"2019","journal-title":"Anal. Biochem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/6865\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:02:49Z","timestamp":1760108569000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/6865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,22]]},"references-count":27,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ijms25136865"],"URL":"https:\/\/doi.org\/10.3390\/ijms25136865","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,22]]}}}