{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T12:02:31Z","timestamp":1774440151900,"version":"3.50.1"},"reference-count":68,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"the Canadian Institutes for Health Research (CIHR)","doi-asserted-by":"publisher","award":["FDN-143213"],"award-info":[{"award-number":["FDN-143213"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"the Canadian Institutes for Health Research (CIHR)","doi-asserted-by":"publisher","award":["PJT-180441"],"award-info":[{"award-number":["PJT-180441"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Quebec Cell, Tissue and Gene Therapy Network\u2014Th\u00e9Cell (a thematic network supported by the Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS))","award":["FDN-143213"],"award-info":[{"award-number":["FDN-143213"]}]},{"name":"Quebec Cell, Tissue and Gene Therapy Network\u2014Th\u00e9Cell (a thematic network supported by the Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS))","award":["PJT-180441"],"award-info":[{"award-number":["PJT-180441"]}]},{"name":"Fondation des Pompiers du Qu\u00e9bec pour les Grands Br\u00fbl\u00e9s (FPQGB)","award":["FDN-143213"],"award-info":[{"award-number":["FDN-143213"]}]},{"name":"Fondation des Pompiers du Qu\u00e9bec pour les Grands Br\u00fbl\u00e9s (FPQGB)","award":["PJT-180441"],"award-info":[{"award-number":["PJT-180441"]}]},{"name":"postdoctoral fellowship from FRQS","award":["FDN-143213"],"award-info":[{"award-number":["FDN-143213"]}]},{"name":"postdoctoral fellowship from FRQS","award":["PJT-180441"],"award-info":[{"award-number":["PJT-180441"]}]},{"name":"studentship from FRQS","award":["FDN-143213"],"award-info":[{"award-number":["FDN-143213"]}]},{"name":"studentship from FRQS","award":["PJT-180441"],"award-info":[{"award-number":["PJT-180441"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The immunogenicity of allogeneic skin fibroblasts in transplantation has been controversial. Whether this controversy comes from a natural heterogeneity among fibroblast subsets or species-specific differences between human and mouse remains to be addressed. In this study, we sought to investigate whether fibroblasts derived from either adult or neonatal human skin tissues could induce different immune responses toward phagocytosis and T cell activation using in vitro co-culture models. Our results indicate that both phagocytosis and T cell proliferation are reduced in the presence of neonatal skin fibroblasts compared to adult skin fibroblasts. We also show that neonatal skin fibroblasts secrete paracrine factors that are responsible for reduced T cell proliferation. In addition, we show that neonatal skin fibroblasts express less class II human leukocyte antigen (HLA) molecules than adult skin fibroblasts after interferon gamma priming, which might also contribute to reduced T cell proliferation. In conclusion, this study supports the use of allogeneic neonatal skin fibroblasts as a readily available cell source for tissue production and transplantation to treat patients with severe injuries.<\/jats:p>","DOI":"10.3390\/ijms25136965","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T05:11:39Z","timestamp":1719465099000},"page":"6965","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Human Neonatal Skin Fibroblast, an Available Cell Source for Tissue Production and Transplantation, Exhibits Low Risk of Immunogenicity In Vitro"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-1144","authenticated-orcid":false,"given":"Brice","family":"Magne","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec City, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en 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Laval, Qu\u00e9bec City, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Quebec City, QC G1J 5B3, Canada"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec City, QC G1E 6W2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin A.","family":"Barbier","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec City, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Quebec City, QC G1J 5B3, Canada"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec City, QC G1E 6W2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Am\u00e9lie","family":"Morissette","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec City, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Quebec City, QC G1J 5B3, Canada"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec City, QC G1E 6W2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Larouche","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec City, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Quebec City, QC G1J 5B3, Canada"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec City, QC G1E 6W2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanel","family":"Beaudoin-Cloutier","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of 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