{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:46:41Z","timestamp":1790812001297,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science and Innovation","award":["PID2022-140302OB-I00"],"award-info":[{"award-number":["PID2022-140302OB-I00"]}]},{"name":"Spanish Ministry of Science and Innovation","award":["2021-SGR-01042"],"award-info":[{"award-number":["2021-SGR-01042"]}]},{"name":"Generalitat de Catalunya","award":["PID2022-140302OB-I00"],"award-info":[{"award-number":["PID2022-140302OB-I00"]}]},{"name":"Generalitat de Catalunya","award":["2021-SGR-01042"],"award-info":[{"award-number":["2021-SGR-01042"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Surface modification of drug-loaded particles with polyethylene glycol (PEG) chains is a powerful tool that promotes better transport of therapeutic agents, provides stability, and avoids their detection by the immune system. In this study, we used a new approach to synthesize a biodegradable poly(ester amide) (PEA) and PEGylating surfactant. These were employed to fabricate micro\/nanoparticles with a core\u2013shell structure. Nanoparticle (NP)-protein interactions and self-assembling were subsequently studied by synchrotron radiation-based FTIR microspectroscopy (SR-FTIRM) and transmission electron microscopy (TEM) techniques. The core\u2013shell structure was identified using IR absorption bands of characteristic chemical groups. Specifically, the stretching absorption band of the secondary amino group (3300 cm\u22121) allowed us to identify the poly(ester amide) core, while the band at 1105 cm\u22121 (C-O-C vibration) was useful to demonstrate the shell structure based on PEG chains. By integration of absorption bands, a 2D intensity map of the particle was built to show a core\u2013shell structure, which was further supported by TEM images.<\/jats:p>","DOI":"10.3390\/ijms25136999","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T05:11:39Z","timestamp":1719465099000},"page":"6999","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["PEGylated Micro\/Nanoparticles Based on Biodegradable Poly(Ester Amides): Preparation and Study of the Core\u2013Shell Structure by Synchrotron Radiation-Based FTIR Microspectroscopy and Electron Microscopy"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0568-9399","authenticated-orcid":false,"given":"Davit","family":"Makharadze","sequence":"first","affiliation":[{"name":"Departament de Enginyeria Qu\u00edmica, Universitat Polit\u00e8cnica de Catalunya, EEBE, Av. Eduard Maristany 10-14, 08019 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temur","family":"Kantaria","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7818-8611","authenticated-orcid":false,"given":"Ibraheem","family":"Yousef","sequence":"additional","affiliation":[{"name":"ALBA Synchrotron Light Facility, Carrer de la Llum 2-26, Cerdanyola del Vall\u00e8s, 08290 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9916-1741","authenticated-orcid":false,"given":"Luis J.","family":"del Valle","sequence":"additional","affiliation":[{"name":"Departament de Enginyeria Qu\u00edmica, Universitat Polit\u00e8cnica de Catalunya, EEBE, Av. Eduard Maristany 10-14, 08019 Barcelona, Spain"},{"name":"Barcelona Research Center in Multiscale Science and Engineering, Universitat Polit\u00e8cnica de Catalunya, Campus Diagonal-Bes\u00f2s, Av. Eduard Maristany 10-14, 08019 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2373-1514","authenticated-orcid":false,"given":"Ramaz","family":"Katsarava","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi 0159, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0640-4474","authenticated-orcid":false,"given":"Jordi","family":"Puiggal\u00ed","sequence":"additional","affiliation":[{"name":"Departament de Enginyeria Qu\u00edmica, Universitat Polit\u00e8cnica de Catalunya, EEBE, Av. Eduard Maristany 10-14, 08019 Barcelona, Spain"},{"name":"Barcelona Research Center in Multiscale Science and Engineering, Universitat Polit\u00e8cnica de Catalunya, Campus Diagonal-Bes\u00f2s, Av. Eduard Maristany 10-14, 08019 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5178","DOI":"10.1038\/s41598-017-05461-3","article-title":"Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line","volume":"7","author":"Azizi","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.addr.2009.11.007","article-title":"Fabrication of gold nanoparticles for targeted therapy in pancreatic cancer","volume":"62","author":"Patra","year":"2010","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/j.biomaterials.2006.09.047","article-title":"Formulation of functionalized PLGA\u2013PEG nanoparticles for in vivo targeted drug delivery","volume":"28","author":"Cheng","year":"2007","journal-title":"Biomaterials"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"19347","DOI":"10.1073\/pnas.1115945108","article-title":"In vivo prevention of arterial restenosis with paclitaxel-encapsulated targeted lipid\u2013polymeric nanoparticles","volume":"108","author":"Chan","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijpharm.2012.10.014","article-title":"Polymeric nanoparticles augment the ocular hypotensive effect of melatonin in rabbits","volume":"440","author":"Musumeci","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7595","DOI":"10.1021\/nn301873v","article-title":"Hybrid Dendrimer Hydrogel\/PLGA Nanoparticle Platform Sustains Drug Delivery for One Week and Antiglaucoma Effects for Four Days Following One-Time Topical Administration","volume":"6","author":"Yang","year":"2012","journal-title":"ACS Nano"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1080\/10717544.2018.1428243","article-title":"Therapeutic strategies and nano-drug delivery applications in management of ageing Alzheimer\u2019s disease","volume":"25","author":"Karthivashan","year":"2018","journal-title":"Drug Deliv."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.phrs.2010.03.005","article-title":"Targeting nanoparticles to cancer","volume":"62","author":"Wang","year":"2010","journal-title":"Pharmacol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2848","DOI":"10.1002\/smll.200901048","article-title":"Anti-inflammatory Properties of Cerium Oxide Nanoparticles","volume":"5","author":"Hirst","year":"2009","journal-title":"Small"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7665","DOI":"10.1016\/j.biomaterials.2012.06.101","article-title":"Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins","volume":"33","author":"Whitmire","year":"2012","journal-title":"Biomaterials"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rahman, M., Laurent, S., Tawil, N., Yahia, L., and Mahmoudi, M. (2013). Protein-Nanoparticle Interactions, Springer.","DOI":"10.1007\/978-3-642-37555-2"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.ejpb.2019.05.006","article-title":"Effect of nanoparticle size and PEGylation on the protein corona of PLGA nanoparticles","volume":"141","author":"Partikel","year":"2019","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/S0005-2728(01)00165-7","article-title":"p-Nitrophenylcarbonyl-PEG-PE-liposomes: Fast and simple attachment of specific ligands, including monoclonal antibodies, to distal ends of PEG chains via p-nitrophenylcarbonyl groups","volume":"1511","author":"Torchilin","year":"2001","journal-title":"Biochim. Et Biophys. Acta (BBA) Biomembr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.biomaterials.2015.11.056","article-title":"Effect of surface properties on liposomal siRNA delivery","volume":"79","author":"Xia","year":"2016","journal-title":"Biomaterials"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/0005-2736(93)90039-3","article-title":"Specific targeting with poly(ethylene glycol)-modified liposomes: Coupling of homing devices to the ends of the polymeric chains combines effective target binding with long circulation times","volume":"1149","author":"Blume","year":"1993","journal-title":"Biochim. Et Biophys. Acta (BBA) Biomembr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1021\/acs.nanolett.0c03756","article-title":"Brush Conformation of Polyethylene Glycol Determines the Stealth Effect of Nanocarriers in the Low Protein Adsorption Regime","volume":"21","author":"Li","year":"2021","journal-title":"Nano Lett."},{"key":"ref_17","first-page":"11","article-title":"The effect of PEG molecular weights on dissolution behavior of simvastatin in solid dispersions","volume":"12","author":"Bolourchian","year":"2013","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6996","DOI":"10.1021\/acsnano.5b01326","article-title":"Surface Functionalization of Nanoparticles with Polyethylene Glycol: Effects on Protein Adsorption and Cellular Uptake","volume":"9","author":"Pelaz","year":"2015","journal-title":"ACS Nano"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1002\/jps.2600830432","article-title":"Distribution and Tissue Uptake of Poly(ethylene glycol) with Different Molecular Weights after Intravenous Administration to Mice","volume":"83","author":"Yamaoka","year":"1994","journal-title":"J. Pharm. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ijpharm.2005.10.010","article-title":"Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles","volume":"307","author":"Owensiii","year":"2006","journal-title":"Int. J. Pharm."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1002\/jbm.820251211","article-title":"Protein adsorption to poly(ethylene oxide) surfaces","volume":"25","author":"Gombotz","year":"1991","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0927-7765(99)00156-3","article-title":"\u2018Stealth\u2019 corona-core nanoparticles surface modified by polyethylene glycol (PEG): Influences of the corona (PEG chain length and surface density) and of the core composition on phagocytic uptake and plasma protein adsorption","volume":"18","author":"Gref","year":"2000","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"120287","DOI":"10.1016\/j.ijpharm.2021.120287","article-title":"Preparation and evaluation of PEG-coated zein nanoparticles for oral drug delivery purposes","volume":"597","author":"Reboredo","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1039\/D1MA01079D","article-title":"Coating liposomes with ring-like PEG: The synthesis and stealth effect of cholesterol\u2013PEG\u2013cholesterol","volume":"3","author":"Wang","year":"2022","journal-title":"Mater. Adv."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3720","DOI":"10.1021\/nl201978c","article-title":"Self-Assembly of Phospholipid\u2013PEG Coating on Nanoparticles through Dual Solvent Exchange","volume":"11","author":"Tong","year":"2011","journal-title":"Nano Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1080\/10601329408545880","article-title":"Design of Nanoscopic Vehicles for Drug Targeting Based on Micellization of Amphiphiuc Block Copolymers","volume":"31","author":"Kataoka","year":"1994","journal-title":"J. Macromol. Sci. Part. A"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"215103","DOI":"10.1088\/0957-4484\/21\/21\/215103","article-title":"Biodegradable self-assembled PEG\u2013PCL\u2013PEG micelles for hydrophobic honokiol delivery: I. Preparation and characterization","volume":"21","author":"Gong","year":"2010","journal-title":"Nanotechnology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.0022-2720.2004.01356.x","article-title":"Possible common biomarkers from FTIR microspectroscopy of cervical cancer and melanoma","volume":"215","author":"Mordechai","year":"2004","journal-title":"J. Microsc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.tibtech.2009.09.001","article-title":"FTIR microspectroscopy for improved prostate cancer diagnosis","volume":"27","author":"Mackanos","year":"2009","journal-title":"Trends Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.jpba.2004.08.010","article-title":"FTIR microscopy as a method for identification of bacterial and fungal infections","volume":"37","author":"Erukhimovitch","year":"2005","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1002\/(SICI)1099-0518(19990215)37:4<391::AID-POLA3>3.0.CO;2-E","article-title":"Amino acid-based bioanalogous polymers. Synthesis, and study of regular poly(ester amide)s based on bis(?-amino acid) ?,?-alkylene diesters, and aliphatic dicarboxylic acids","volume":"37","author":"Katsarava","year":"1999","journal-title":"J. Polym. Sci. A Polym. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1126\/science.8128245","article-title":"Biodegradable Long-Circulating Polymeric Nanospheres","volume":"263","author":"Gref","year":"1994","journal-title":"Science"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1039\/c0py00025f","article-title":"Conjugation of RAFT-generated polymers to proteins by two consecutive thiol\u2013ene reactions","volume":"1","author":"Li","year":"2010","journal-title":"Polym. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/ajoc.201800639","article-title":"Thiol\u2212Ene Reaction: Synthetic Aspects and Mechanistic Studies of an Anti-Markovnikov-Selective Hydrothiolation of Olefins","volume":"8","author":"Sinha","year":"2019","journal-title":"Asian J. Org. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1021\/bm100290n","article-title":"Rapid Approach to Biobased Telechelics through Two One-Pot Thiol\u2212Ene Click Reactions","volume":"11","author":"Lluch","year":"2010","journal-title":"Biomacromolecules"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1021\/cm402180t","article-title":"The Thiol-Michael Addition Click Reaction: A Powerful and Widely Used Tool in Materials Chemistry","volume":"26","author":"Nair","year":"2014","journal-title":"Chem. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1351\/PAC-REC-10-12-04","article-title":"Terminology for biorelated polymers and applications (IUPAC Recommendations 2012)","volume":"84","author":"Vert","year":"2012","journal-title":"Pure Appl. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0304-4165(93)90105-H","article-title":"An investigation of the filtration capacity and the fate of large filtered sterically-stabilized microspheres in rat spleen","volume":"1157","author":"Moghimi","year":"1993","journal-title":"Biochim. Et. Biophys. Acta (BBA) Gen. Subj."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1038\/nbt1340","article-title":"Renal clearance of quantum dots","volume":"25","author":"Choi","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"703","DOI":"10.2217\/17435889.3.5.703","article-title":"Clearance properties of nano-sized particles and molecules as imaging agents: Considerations and caveats","volume":"3","author":"Longmire","year":"2008","journal-title":"Nanomedicine"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13572","DOI":"10.1021\/ja1035607","article-title":"Selective recognition and extraction of the uranyl ion","volume":"132","author":"Sather","year":"2010","journal-title":"J. Am. Chem. Soc."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Katsarava, R. (2021). Pseudo-Proteins and Related Synthetic Amino Acid-Based Polymers Promising for Constructing Artificial Vaccines. Synthetic Peptide Vaccine Models, CRC Press.","DOI":"10.1201\/9781003144533-10"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1016\/0378-5173(89)90281-0","article-title":"Nanocapsule formation by interfacial polymer deposition following solvent displacement","volume":"55","author":"Fessi","year":"1989","journal-title":"Int. J. Pharm."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kantaria, T., Kantaria, T., Kobauri, S., Ksovreli, M., Kachlishvili, T., Kulikova, N., Tugushi, D., and Katsarava, R. (2016). Biodegradable Nanoparticles Made of Amino-Acid-Based Ester Polymers: Preparation, Characterization, and In Vitro Biocompatibility Study. Appl. Sci., 6.","DOI":"10.3390\/app6120444"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1021\/acsomega.8b02977","article-title":"Library of Cationic Polymers Composed of Polyamines and Arginine as Gene Transfection Agents","volume":"4","author":"Zavradashvili","year":"2019","journal-title":"ACS Omega"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/6999\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:05:08Z","timestamp":1760108708000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/6999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,26]]},"references-count":45,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ijms25136999"],"URL":"https:\/\/doi.org\/10.3390\/ijms25136999","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,26]]}}}