{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:47:45Z","timestamp":1789714065494,"version":"4.0.1"},"reference-count":104,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"L\u2019ANRS Maladies infectieuses \u00e9mergentes","award":["ANRS0379"],"award-info":[{"award-number":["ANRS0379"]}]},{"name":"L\u2019ANRS Maladies infectieuses \u00e9mergentes","award":["ANRS0386b"],"award-info":[{"award-number":["ANRS0386b"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The development of direct-acting antivirals (DAAs) against hepatitis C virus (HCV) has revolutionized the management of this pathology, as their use allows viral elimination in a large majority of patients. Nonetheless, HCV remains a major public health problem due to the multiple challenges associated with its diagnosis, treatment availability and development of a prophylactic vaccine. Moreover, HCV-cured patients still present an increased risk of developing hepatic complications such as hepatocellular carcinoma. In the present review, we aim to summarize the impact that HCV infection has on a wide variety of peripheral and intrahepatic cell populations, the alterations that remain following DAA treatment and the potential molecular mechanisms implicated in their long-term persistence. Finally, we consider how recent developments in single-cell multiomics could refine our understanding of this disease in each specific intrahepatic cell population and drive the field to explore new directions for the development of chemo-preventive strategies.<\/jats:p>","DOI":"10.3390\/ijms25137073","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T13:26:43Z","timestamp":1719494803000},"page":"7073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["After the Storm: Persistent Molecular Alterations Following HCV Cure"],"prefix":"10.3390","volume":"25","author":[{"given":"Coline","family":"Seurre","sequence":"first","affiliation":[{"name":"INSERM U1052, CNRS 5286, Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69434 Lyon, France"},{"name":"The Lyon Hepatology Institute EVEREST, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando Andres","family":"Roca Suarez","sequence":"additional","affiliation":[{"name":"INSERM U1052, CNRS 5286, Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69434 Lyon, France"},{"name":"The Lyon Hepatology Institute EVEREST, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5588-5465","authenticated-orcid":false,"given":"Barbara","family":"Testoni","sequence":"additional","affiliation":[{"name":"INSERM U1052, CNRS 5286, Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69434 Lyon, France"},{"name":"The Lyon Hepatology Institute EVEREST, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2245-0083","authenticated-orcid":false,"given":"Fabien","family":"Zoulim","sequence":"additional","affiliation":[{"name":"INSERM U1052, CNRS 5286, Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69434 Lyon, France"},{"name":"The Lyon Hepatology Institute EVEREST, 69003 Lyon, France"},{"name":"Hospices Civils de Lyon, 69002 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0376-7779","authenticated-orcid":false,"given":"Boyan","family":"Grigorov","sequence":"additional","affiliation":[{"name":"INSERM U1052, CNRS 5286, Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69434 Lyon, France"},{"name":"The Lyon Hepatology Institute EVEREST, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1126\/science.2523562","article-title":"Isolation of a CDNA Clone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis Genome","volume":"244","author":"Choo","year":"1989","journal-title":"Science"},{"key":"ref_2","unstructured":"European Association for the Study of the Liver. 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