{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:28:33Z","timestamp":1790310513835,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Nacional de Cardiologia Ignacio Chavez"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Type 2 diabetes mellitus (T2DM) is associated with various complications, including diabetic foot, which can lead to significant morbidity and mortality. Non-healing foot ulcers in diabetic patients are a major risk factor for infections and amputations. Despite conventional treatments, which have limited efficacy, there is a need for more effective therapies. MicroRNAs (miRs) are small non-coding RNAs that play a role in gene expression and have been implicated in diabetic wound healing. miR expression was analyzed through RT-qPCR in 41 diabetic foot Mexican patients and 50 controls. Diabetic foot patients showed significant increases in plasma levels of miR-17-5p (p = 0.001), miR-191-5p (p = 0.001), let-7e-5p (p = 0.001), and miR-33a-5p (p = 0.005) when compared to controls. Elevated levels of miR-17, miR-191, and miR-121 correlated with higher glucose levels in patients with diabetic foot ulcers (r = 0.30, p = 0.004; r = 0.25, p = 0.01; and r = 0.21, p = 0.05, respectively). Levels of miR-17 showed the highest diagnostic potential (AUC 0.903, p = 0.0001). These findings underscore the possible role of these miRs in developing diabetes complications. Our study suggests that high miR-17, miR-191, and miR-121 expression is strongly associated with higher glucose levels and the development of diabetic foot ulcers.<\/jats:p>","DOI":"10.3390\/ijms25137078","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T13:26:43Z","timestamp":1719494803000},"page":"7078","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Analysis of Circulating miRNA Expression Profiles in Type 2 Diabetes Patients with Diabetic Foot Complications"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1819-8444","authenticated-orcid":false,"given":"Giovanny","family":"Fuentevilla-Alvarez","sequence":"first","affiliation":[{"name":"Endocrinology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1. Col. Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1332-2888","authenticated-orcid":false,"given":"Mar\u00eda Elena","family":"Soto","sequence":"additional","affiliation":[{"name":"Research Direction, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1. Col. Secci\u00f3n XVI, Mexico City 14080, Mexico"},{"name":"Cardiovascular Line in American British Cowdary (ABC) Medical Center, Sur 136 No. 116 Col. Las Am\u00e9ricas, Mexico City 01120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Jaziel","family":"Robles-Herrera","sequence":"additional","affiliation":[{"name":"Phisiology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1. Col. Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7916-5163","authenticated-orcid":false,"given":"Gilberto","family":"Vargas-Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Research Direction, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1. Col. Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reyna","family":"S\u00e1mano","sequence":"additional","affiliation":[{"name":"Coordinaci\u00f3n de Nutrici\u00f3n y Bioprogramaci\u00f3n, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Enrique","family":"Meza-Toledo","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional (IPN), Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6806-3484","authenticated-orcid":false,"given":"Claudia","family":"Huesca-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Phisiology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1. Col. Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Gamboa","sequence":"additional","affiliation":[{"name":"Phisiology Department, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1. Col. Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.tcm.2018.08.002","article-title":"MicroRNAs in diabetic wound healing: Pathophysiology and therapeutic opportunities","volume":"29","author":"Ozdemir","year":"2019","journal-title":"Trends. Cardiovasc. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s13643-017-0488-5","article-title":"Epidemiology of diabetic foot disease and diabetes-related lower-extremity amputation in Australia: A systematic review protocol","volume":"6","author":"Baba","year":"2017","journal-title":"Syst. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.mcna.2013.03.007","article-title":"The Pathway to Foot Ulceration in Diabetes","volume":"97","author":"Boulton","year":"2013","journal-title":"Med. Clin. N. Am."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.arcmed.2018.04.005","article-title":"10 years Analysis of Diabetes-related Major Lower Extremity Amputations in Mexico","volume":"49","year":"2018","journal-title":"Arch. Med. Res."},{"key":"ref_5","first-page":"284","article-title":"Pie diab\u00e9tico en M\u00e9xico: Factores de riesgo para mortalidad posterior a una amputaci\u00f3n mayor, a 5 a\u00f1os, en un hospital de salud p\u00fablica de segundo nivel","volume":"89","year":"2021","journal-title":"Cirug\u00eda Cir."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1742-481X.2007.00392.x","article-title":"Guest Editorial: Are diabetes-related wounds and amputations worse than cancer?","volume":"4","author":"Armstrong","year":"2007","journal-title":"Int. Wound J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1210\/er.2003-0012","article-title":"Diabetic Cardiomyopathy: Evidence, Mechanisms, and Therapeutic Implications","volume":"25","author":"Fang","year":"2004","journal-title":"Endocr. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1152\/physrev.00030.2020","article-title":"Obesity cardiomyopathy: Evidence, mechanisms, and therapeutic implications","volume":"101","author":"Ren","year":"2021","journal-title":"Physiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.4239\/wjd.v13.i12.1001","article-title":"Non-coding RNAs: Role in diabetic foot and wound healing","volume":"13","author":"Tang","year":"2022","journal-title":"World J. Diabetes"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1080\/10717544.2021.2023699","article-title":"Milk exosomes-mediated miR-31-5p delivery accelerates diabetic wound healing through promoting angiogenesis","volume":"29","author":"Yan","year":"2022","journal-title":"Drug Deliv."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Petkovic, M., S\u00f8rensen, A.E., Leal, E.C., Carvalho, E., and Dalgaard, L.T. (2020). Mechanistic Actions of microRNAs in Diabetic Wound Healing. Cells, 9.","DOI":"10.3390\/cells9102228"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ohtsuka, M., Iwamoto, K., Naito, A., Imasato, M., Hyuga, S., Nakahara, Y., Mikamori, M., Furukawa, K., Moon, J., and Asaoka, T. (2021). Circulating MicroRNAs in Gastrointestinal Cancer. Cancers, 13.","DOI":"10.3390\/cancers13133348"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/ijo.2015.170","article-title":"MicroRNAs as regulators of metabolic disease: Pathophysiologic significance and emerging role as biomarkers and therapeutics","volume":"40","author":"Deiuliis","year":"2016","journal-title":"Int. J. Obes."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"804","DOI":"10.2337\/dbi19-0016","article-title":"MicroRNA Networks in Pancreatic Islet Cells: Normal Function and Type 2 Diabetes","volume":"69","author":"Eliasson","year":"2020","journal-title":"Diabetes"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8611","DOI":"10.1038\/s41598-023-35836-8","article-title":"Circulating miRNA expression in long-standing type 1 diabetes mellitus","volume":"13","author":"Lambert","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8605","DOI":"10.1002\/jcp.26576","article-title":"Let-7e inhibits TNF-\u03b1 expression by targeting the methyl transferase EZH2 in DENV2-infected THP-1 cells","volume":"233","author":"Zhang","year":"2018","journal-title":"J. Cell Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.scr.2012.11.007","article-title":"miR-17-5p and miR-106a are involved in the balance between osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells","volume":"10","author":"Li","year":"2013","journal-title":"Stem. Cell Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1126\/science.1189862","article-title":"MiR-33 Contributes to the Regulation of Cholesterol Homeostasis","volume":"328","author":"Rayner","year":"2010","journal-title":"Science"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/s41371-018-0102-x","article-title":"Increased expression of miR-33a in monocytes from Mexican hypertensive patients in elevated carotid intima-media thickness","volume":"32","author":"Soto","year":"2018","journal-title":"J. Hum. Hypertens."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"27914","DOI":"10.1038\/srep27914","article-title":"miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.biocel.2014.01.005","article-title":"MiR-144 regulates hematopoiesis and vascular development by targeting meis1 during zebrafish development","volume":"49","author":"Su","year":"2014","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/S0140-6736(05)67700-8","article-title":"Wound healing and its impairment in the diabetic foot","volume":"366","author":"Falanga","year":"2005","journal-title":"Lancet"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/s41580-021-00354-w","article-title":"MicroRNA-mediated regulation of glucose and lipid metabolism","volume":"22","author":"Agbu","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1161\/CIRCRESAHA.116.309528","article-title":"MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol","volume":"120","author":"Nishiga","year":"2017","journal-title":"Circ. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4334","DOI":"10.1172\/JCI81676","article-title":"MicroRNA-33\u2013dependent regulation of macrophage metabolism directs immune cell polarization in atherosclerosis","volume":"125","author":"Ouimet","year":"2015","journal-title":"J. Clin. Investig."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1758","DOI":"10.1096\/fj.10-179267","article-title":"Cholesterol regulation of receptor-interacting protein 140 via microRNA-33 in inflammatory cytokine production","volume":"25","author":"Ho","year":"2011","journal-title":"FASEB J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1016\/j.celrep.2018.01.074","article-title":"Genetic Ablation of miR-33 Increases Food Intake, Enhances Adipose Tissue Expansion, and Promotes Obesity and Insulin Resistance","volume":"22","author":"Price","year":"2018","journal-title":"Cell Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1007\/s11033-021-06425-7","article-title":"Serum microRNA-33 levels in pre-diabetic and diabetic patients","volume":"48","author":"Shahouzehi","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1172\/JCI57275","article-title":"Antagonism of miR-33 in mice promotes reverse cholesterol transport and regression of atherosclerosis","volume":"121","author":"Rayner","year":"2011","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1002\/kjm2.12610","article-title":"MiR-33-5p alleviates spinal cord injury in rats and protects <scp>PC12<\/scp> cells from lipopolysaccharide-induced apoptosis","volume":"39","author":"Li","year":"2023","journal-title":"Kaohsiung J. Med. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Pan, Q., Xu, X., He, W., Wang, Y., Xiang, Z., Jin, X., Tang, Q., Zhao, T., and Ma, X. (2023). Enrichment of miR-17-5p enhances the protective effects of EPC-EXs on vascular and skeletal muscle injury in a diabetic hind limb ischemia model. Biol. Res., 56.","DOI":"10.1186\/s40659-023-00418-5"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.yexcr.2019.04.039","article-title":"Glucose impairs angiogenesis and promotes ventricular remodelling following myocardial infarction via upregulation of microRNA-17","volume":"381","author":"Yan","year":"2019","journal-title":"Exp. Cell Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.ijcard.2019.05.036","article-title":"Postpartum profiling of microRNAs involved in pathogenesis of cardiovascular\/cerebrovascular diseases in women exposed to pregnancy-related complications","volume":"291","author":"Hromadnikova","year":"2019","journal-title":"Int. J. Cardiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hromadnikova, I., Kotlabova, K., Hympanova, L., and Krofta, L. (2015). Cardiovascular and Cerebrovascular Disease Associated microRNAs Are Dysregulated in Placental Tissues Affected with Gestational Hypertension, Preeclampsia and Intrauterine Growth Restriction. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0138383"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1080\/09537104.2020.1810223","article-title":"Contribution of platelet-derived microRNAs to serum microRNAs in healthy men","volume":"32","author":"Wakabayashi","year":"2021","journal-title":"Platelets"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1161\/ATVBAHA.114.305048","article-title":"Impairment of Wound Healing in Patients With Type 2 Diabetes Mellitus Influences Circulating MicroRNA Patterns via Inflammatory Cytokines","volume":"35","author":"Dangwal","year":"2015","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3321","DOI":"10.1096\/fj.201601263R","article-title":"miR-191 suppresses angiogenesis by activation of NF-kB signaling","volume":"31","author":"Gu","year":"2017","journal-title":"FASEB J."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Hsu, A., Chen, S.-J., Chang, Y.-S., Chen, H.-C., and Chu, P.-H. (2014). Systemic Approach to Identify Serum microRNAs as Potential Biomarkers for Acute Myocardial Infarction. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/418628"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s40001-015-0148-y","article-title":"Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction","volume":"20","author":"Li","year":"2015","journal-title":"Eur. J. Med. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cjca.2020.03.018","article-title":"Circulating miRNAs Related to Long-term Adverse Cardiovascular Events in STEMI Patients: A Nested Case-Control Study","volume":"37","author":"Yang","year":"2021","journal-title":"Can. J. Cardiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1161\/CIRCRESAHA.110.226357","article-title":"Plasma MicroRNA Profiling Reveals Loss of Endothelial MiR-126 and Other MicroRNAs in Type 2 Diabetes","volume":"107","author":"Zampetaki","year":"2010","journal-title":"Circ. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1007\/s00592-014-0617-8","article-title":"Serum miR-23a, a potential biomarker for diagnosis of pre-diabetes and type 2 diabetes","volume":"51","author":"Yang","year":"2014","journal-title":"Acta Diabetol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s00592-016-0915-4","article-title":"MicroRNA-126 and micro-\/macrovascular complications of type 1 diabetes in the EURODIAB Prospective Complications Study","volume":"54","author":"Barutta","year":"2017","journal-title":"Acta Diabetol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0194-y","article-title":"A plasma circulating miRNAs profile predicts type 2 diabetes mellitus and prediabetes: From the CORDIOPREV study","volume":"50","author":"Camargo","year":"2018","journal-title":"Exp. Mol. Med."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"De Rosa, S., Arcidiacono, B., Chiefari, E., Brunetti, A., Indolfi, C., and Foti, D.P. (2018). Type 2 Diabetes Mellitus and Cardiovascular Disease: Genetic and Epigenetic Links. Front. Endocrinol., 9.","DOI":"10.3389\/fendo.2018.00002"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ueta, M., Nishigaki, H., Komai, S., Mizushima, K., Tamagawa-Mineoka, R., Naito, Y., Katoh, N., Sotozono, C., and Kinoshita, S. (2022). Positive regulation of innate immune response by miRNA-let-7a-5p. Front. Genet., 13.","DOI":"10.3389\/fgene.2022.1025539"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6051874","DOI":"10.1155\/2017\/6051874","article-title":"miR-Let7A Controls the Cell Death and Tight Junction Density of Brain Endothelial Cells under High Glucose Condition","volume":"2017","author":"Song","year":"2017","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8887","DOI":"10.1038\/s41598-019-43793-4","article-title":"Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes","volume":"9","author":"Mononen","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Karolina, D.S., Armugam, A., Tavintharan, S., Wong, M.T.K., Lim, S.C., Sum, C.F., and Jeyaseelan, K. (2011). MicroRNA 144 Impairs Insulin Signaling by Inhibiting the Expression of Insulin Receptor Substrate 1 in Type 2 Diabetes Mellitus. PLoS ONE, 6.","DOI":"10.1371\/annotation\/698b7123-174f-4a09-95c9-fd6f5017d622"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Okamura, T., Okada, H., Hashimoto, Y., Majima, S., Senmaru, T., Nakanishi, N., Asano, M., Yamazaki, M., Hamaguchi, M., and Fukui, M. (2021). Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.791363"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"111190","DOI":"10.1016\/j.intimp.2023.111190","article-title":"Diabetic and nondiabetic BMSC\u2013derived exosomes affect bone regeneration via regulating miR-17-5p\/SMAD7 axis","volume":"125","author":"Li","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1016\/j.drudis.2022.05.003","article-title":"Key regulatory miRNAs in lipid homeostasis: Implications for cardiometabolic diseases and development of novel therapeutics","volume":"27","author":"Khan","year":"2022","journal-title":"Drug Discov. Today"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1080\/21623945.2020.1798632","article-title":"Circulating microRNA changes in patients with impaired glucose regulation","volume":"9","author":"Fachim","year":"2020","journal-title":"Adipocyte"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ortega, R., Liu, B., and Persaud, S.J. (2023). Effects of miR-33 Deficiency on Metabolic and Cardiovascular Diseases: Implications for Therapeutic Intervention. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241310777"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"World Medical Association (2013). World Medical Association Declaration of Helsinki. JAMA, 310, 2191.","DOI":"10.1001\/jama.2013.281053"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1016\/S0022-2275(20)31581-9","article-title":"High prevalence of low HDL cholesterol concentrations and mixed hyperlipidemia in a Mexican nationwide survey","volume":"42","author":"Olaiz","year":"2001","journal-title":"J. Lipid Res."},{"key":"ref_57","unstructured":"(2012). Mexican Official Norm NOM-037-SSA2-2012. For the prevention, treatment and control of dyslipidemias. Rev. Mex. Cardiol., 23, 91\u2013124."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gkaa467","article-title":"miRNet 2.0: Network-based visual analytics for miRNA functional analysis and systems biology","volume":"48","author":"Chang","year":"2020","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/7078\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:06:36Z","timestamp":1760108796000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/7078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,27]]},"references-count":59,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ijms25137078"],"URL":"https:\/\/doi.org\/10.3390\/ijms25137078","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,27]]}}}