{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T14:35:21Z","timestamp":1776436521604,"version":"3.51.2"},"reference-count":83,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Education and Science through a subsidy for the University of Agriculture in Krakow","award":["21\u201347159L"],"award-info":[{"award-number":["21\u201347159L"]}]},{"name":"Polish Ministry of Education and Science through a subsidy for the University of Agriculture in Krakow","award":["LM2023064"],"award-info":[{"award-number":["LM2023064"]}]},{"name":"Czech Science Foundation (GA\u010cR)","award":["21\u201347159L"],"award-info":[{"award-number":["21\u201347159L"]}]},{"name":"Czech Science Foundation (GA\u010cR)","award":["LM2023064"],"award-info":[{"award-number":["LM2023064"]}]},{"name":"METRO FOOD-CZ research infrastructure project","award":["21\u201347159L"],"award-info":[{"award-number":["21\u201347159L"]}]},{"name":"METRO FOOD-CZ research infrastructure project","award":["LM2023064"],"award-info":[{"award-number":["LM2023064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Foods enriched with insects can potentially prevent several health disorders, including cardiovascular diseases, by reducing inflammation and improving antioxidant status. In this study, Tenebrio molitor and Gryllus assimilis were selected to determine the effect on the development of atherosclerosis in ApoE\/LDLR\u2212\/\u2212 mice. Animals were fed AIN-93G-based diets (control) with 10% Tenebrio molitor (TM) and 10% Gryllus assimilis (GA) for 8 weeks. The nutritional value as well as antioxidant activity of selected insects were determined. The lipid profile, liver enzyme activity, and the fatty acid composition of liver and adipose tissue of model mice were evaluated. Quantitative analysis of atherosclerotic lesions in the entire aorta was performed using the en face method, and for aortic roots, the cross-section method was used. The antioxidant status of the GA cricket was significantly higher compared to the TM larvae. The results showed that the area of atherosclerosis (en face method) was not significantly different between groups. Dietary GA reduced plaque formation in the aortic root; additionally, significant differences were observed in sections at 200 and 300 \u00b5m compared to other groups. Furthermore, liver enzyme ALT activity was lower in insect-fed groups compared to the control group. The finding suggests that a diet containing edible insect GA potentially prevents atherosclerotic plaque development in the aortic root, due to its high antioxidant activity.<\/jats:p>","DOI":"10.3390\/ijms25137256","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T12:58:42Z","timestamp":1719925122000},"page":"7256","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Diets with the Addition of Edible Insects on the Development of Atherosclerotic Lesions in ApoE\/LDLR\u2212\/\u2212 Mice"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8103-1216","authenticated-orcid":false,"given":"Hayat","family":"Hassen","sequence":"first","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, A. Mickiewicz Av. 21, 31-120 Krak\u00f3w, Poland"},{"name":"Department of Human Nutrition, Faculty of Chemical and Food Engineering, Bahir Dar Institute of Technology, Bahir Dar P.O. Box 26, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4591-9323","authenticated-orcid":false,"given":"Petra","family":"\u0160kvorov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kam\u00fdck\u00e1 129, 165 00 Praha-Suchdol, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5655-6046","authenticated-orcid":false,"given":"Kshitiz","family":"Pokhrel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kam\u00fdck\u00e1 129, 165 00 Praha-Suchdol, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3430-1392","authenticated-orcid":false,"given":"Martin","family":"Kulma","sequence":"additional","affiliation":[{"name":"Department of Zoology and Fisheries, Czech University of Life Sciences Prague, Kam\u00fdck\u00e1 129, 165 00 Praha-Suchdol, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Pi\u0105tkowska","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, A. Mickiewicz Av. 21, 31-120 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata B.","family":"Kostogrys","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, A. Mickiewicz Av. 21, 31-120 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-7239","authenticated-orcid":false,"given":"Lenka","family":"Kou\u0159imsk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kam\u00fdck\u00e1 129, 165 00 Praha-Suchdol, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9166-1805","authenticated-orcid":false,"given":"Tomasz","family":"Tarko","sequence":"additional","affiliation":[{"name":"Department of Fermentation Technology and Microbiology, Faculty of Food Technology, University of Agriculture in Krakow, A. Mickiewicz Av. 21, 31-120 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7379-4054","authenticated-orcid":false,"given":"Magdalena","family":"Franczyk-\u017bar\u00f3w","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, A. Mickiewicz Av. 21, 31-120 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1016\/j.jacc.2020.11.021","article-title":"The global burden of cardiovascular diseases and risks: A compass for global action","volume":"76","author":"Roth","year":"2020","journal-title":"J. Am. Coll. 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