{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T06:30:54Z","timestamp":1770532254073,"version":"3.49.0"},"reference-count":42,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T00:00:00Z","timestamp":1720137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Sichuan Science and Technology Program","doi-asserted-by":"publisher","award":["2023YFS0342"],"award-info":[{"award-number":["2023YFS0342"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Sichuan Science and Technology Program","doi-asserted-by":"publisher","award":["E1D3080001"],"award-info":[{"award-number":["E1D3080001"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010822","name":"Chengdu-CAS Science and Technology Cooperation Program","doi-asserted-by":"publisher","award":["2023YFS0342"],"award-info":[{"award-number":["2023YFS0342"]}],"id":[{"id":"10.13039\/501100010822","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010822","name":"Chengdu-CAS Science and Technology Cooperation Program","doi-asserted-by":"publisher","award":["E1D3080001"],"award-info":[{"award-number":["E1D3080001"]}],"id":[{"id":"10.13039\/501100010822","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hyaluronidase possesses the capacity to degrade high-molecular-weight hyaluronic acid into smaller fragments, subsequently initiating a cascade of inflammatory responses and activating dendritic cells. In cases of bacterial infections, substantial quantities of HAase are generated, potentially leading to severe conditions such as cellulitis. Inhibiting hyaluronidase activity may offer anti-inflammatory benefits. Salvia miltiorrhiza Bunge, a traditional Chinese medicine, has anti-inflammatory properties. However, its effects on skin inflammation are not well understood. This study screened and evaluated the active components of S. miltiorrhiza that inhibit skin inflammation, using ligand fishing, enzyme activity assays, drug combination analysis, and molecular docking. By combining magnetic nanomaterials with hyaluronidase functional groups, we immobilized hyaluronidase on magnetic nanomaterials for the first time in the literature. We then utilized an immobilized enzyme to specifically adsorb the ligand; two ligands were identified as salvianolic acid B and rosmarinic acid by HPLC analysis after desorption of the dangling ligands, to complete the rapid screening of potential anti-inflammatory active ingredients in S. miltiorrhiza roots. The median-effect equation and combination index results indicated that their synergistic inhibition of hyaluronidase at a fixed 3:2 ratio was enhanced with increasing concentrations. Kinetic studies revealed that they acted as mixed-type inhibitors of hyaluronidase. Salvianolic acid B had Ki and Kis values of 0.22 and 0.96 \u03bcM, respectively, while rosmarinic acid had values of 0.54 and 4.60 \u03bcM. Molecular docking revealed that salvianolic acid B had a higher affinity for hyaluronidase than rosmarinic acid. In addition, we observed that a 3:2 combination of SAB and RA significantly decreased the secretion of TNF-\u03b1, IL-1, and IL-6 inflammatory cytokines in UVB-irradiated HaCaT cells. These findings identify salvianolic acid B and rosmarinic acid as key components with the potential to inhibit skin inflammation, as found in S. miltiorrhiza. This research is significant for developing skin inflammation treatments. It demonstrates the effectiveness and broad applicability of the magnetic nanoparticle-based ligand fishing approach for screening enzyme inhibitors derived from herbal extracts.<\/jats:p>","DOI":"10.3390\/ijms25137369","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T12:21:03Z","timestamp":1720441263000},"page":"7369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid Screening of Chemical Components in Salvia miltiorrhiza with the Potential to Inhibit Skin Inflammation"],"prefix":"10.3390","volume":"25","author":[{"given":"Kehang","family":"He","sequence":"first","affiliation":[{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yikao","family":"Hu","sequence":"additional","affiliation":[{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolin","family":"Bai","sequence":"additional","affiliation":[{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9814-9716","authenticated-orcid":false,"given":"Xun","family":"Liao","sequence":"additional","affiliation":[{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118006","DOI":"10.1016\/j.carbpol.2021.118006","article-title":"Hyaluronic acid in ocular drug delivery","volume":"264","author":"Zhang","year":"2021","journal-title":"Carbohyd. Polym."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.addr.2007.08.008","article-title":"Hyaluronan-dependent pericellular matrix","volume":"59","author":"Evanko","year":"2007","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/s41563-019-0462-9","article-title":"Hyaluronic acid-bilirubin nanomedicine for targeted modulation of dysregulated intestinal barrier, microbiome and immune responses in colitis","volume":"19","author":"Lee","year":"2020","journal-title":"Nat. Mater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"127e","DOI":"10.1097\/PRS.0b013e3182a4c282","article-title":"Changing Role of Hyaluronidase in Plastic Surgery","volume":"133","author":"Bailey","year":"2014","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kobayashi, T., Chanmee, T., and Itano, N. (2020). Hyaluronan: Metabolism and Function. Biomolecules, 10.","DOI":"10.3390\/biom10111525"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4419","DOI":"10.1016\/j.ejmech.2011.07.014","article-title":"Design of benzimidazole- and benzoxazole-2-thione derivatives as inhibitors of bacterial hyaluronan lyase","volume":"46","author":"Braun","year":"2011","journal-title":"Eur. J. Med. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1016\/j.bmcl.2016.04.082","article-title":"Interpreting the behavior of concentrationinhibitor as inhibitor inhibitoric Surgery. ponses in colitturbed assay conditions","volume":"26","author":"Tomohara","year":"2016","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"107291","DOI":"10.1016\/j.bioorg.2024.107291","article-title":"Modular preparation of biphenyl triazoles via click chemistry as non-competitive hyaluronidase inhibitors","volume":"146","author":"Qin","year":"2024","journal-title":"Bioorganic Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1080\/14786419.2018.1519708","article-title":"Anti-inflammatory activity of saponins from roots of Impatiens parviflora DC","volume":"34","author":"Grabowska","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.2174\/092986709788453078","article-title":"Hyaluronidase Inhibitors: A Biological and Therapeutic Perspective","volume":"16","author":"Girish","year":"2009","journal-title":"Curr. Med. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4100","DOI":"10.1016\/j.bmcl.2005.06.012","article-title":"Synthesis of benzoyl phenyl benzoates as effective inhibitors for phospholipase A2 and hyaluronidase enzymes","volume":"15","author":"Khanum","year":"2005","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1055\/s-2005-871255","article-title":"Sulphated Oligosaccharides as Inhibitors of Hyaluronidases from Bovine Testis, Bee Venom and Streptococcus agalactiae","volume":"71","author":"Salmen","year":"2005","journal-title":"Planta Medica"},{"key":"ref_13","first-page":"158","article-title":"A glycoprotein from a folk medicinal plant, Withania somnifera, inhibits hyaluronidase activity of snake venoms","volume":"143","author":"Machiah","year":"2006","journal-title":"Comp. Biochem. Phys. C"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mohamed, E.M., Hetta, M.H., Rateb, M.E., Selim, M.A., Aboulmagd, A.M., Badria, F.A., Abdelmohsen, U.R., Alhadrami, H.A., and Hassan, H.M. (2020). Bioassay-Guided Isolation, Metabolic Profiling, and Docking Studies of Hyaluronidase Inhibitors from Ravenala madagascariensis. Molecules, 25.","DOI":"10.3390\/molecules25071714"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Chaiyana, W., Chansakaow, S., Intasai, N., Kiattisin, K., Lee, K.H., Lin, W.C., Lue, S.C., and Leelapornpisid, P. (2020). Chemical Constituents, Antioxidant, Anti-MMPs, and Anti-Hyaluronidase Activities of Thunbergia laurifolia Lindl. Leaf Extracts for Skin Aging and Skin Damage Prevention. Molecules, 25.","DOI":"10.3390\/molecules25081923"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1021\/acs.jnatprod.1c00649","article-title":"Phytochemical Investigation of the Roots of Ipomoea asarifolia and Antiproliferative Activity of the Isolated Compounds against Multiple Myeloma Cells","volume":"85","author":"Saraux","year":"2021","journal-title":"J. Nat. Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1021\/acs.jnatprod.1c00977","article-title":"Tigliane Diterpenoids with Larvicidal, Antifungal, and \u03b1-Glucosidase Inhibitory Activities from Croton damayeshu","volume":"85","author":"Jiang","year":"2022","journal-title":"J. Nat. Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1039\/c2an15824h","article-title":"A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine","volume":"137","author":"Long","year":"2012","journal-title":"Analyst"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1039\/C3AY41295D","article-title":"Enrichment and analysis of typically persistent organic pollutants at trace level using micro-solid phase extraction based on titanium dioxide nanotube arrays","volume":"6","author":"Zhou","year":"2014","journal-title":"Anal. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"121648","DOI":"10.1016\/j.talanta.2020.121648","article-title":"Application of magnetic nanomaterials in magnetic in-tube solid-phase microextraction\u2014ScienceDirect","volume":"221","author":"Safari","year":"2020","journal-title":"Talanta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2904","DOI":"10.1016\/j.materresbull.2007.12.006","article-title":"Fabrication of nanocomposite particles with superparamagnetic and luminescent functionalities","volume":"43","author":"Liu","year":"2008","journal-title":"Mater. Res. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1021\/cm960157j","article-title":"Synthesis and Characterization of Nanometer-Size Fe3O4 and \u03b3-Fe2O3 Particles","volume":"8","author":"Kang","year":"1996","journal-title":"Chem Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"10679","DOI":"10.1021\/jf503687e","article-title":"Lipase ligands in Nelumbo nucifera leaves and study of their binding mechanism","volume":"62","author":"Zhu","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1002\/jssc.201801255","article-title":"ligand fishing of anti-neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B","volume":"42","author":"Wu","year":"2019","journal-title":"J. Sep. Sci."},{"key":"ref_25","first-page":"152","article-title":"Analysis and comparison of the levels of bioactive components in roots of Salvia miltiorrhiza Bunge from different germplasms by high-performance liquid chromatography","volume":"7","author":"Lu","year":"2013","journal-title":"Aust. J. Crop. Sci."},{"key":"ref_26","first-page":"18","article-title":"Rosmarinic Acid, the Active Component of Salvia miltiorrhizae, improves gliquidone transport by regulating the expression and function of P-gp and BCRP in Caco-2 Cells","volume":"75","author":"Li","year":"2020","journal-title":"Pharmazie"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0021-9673(01)01441-8","article-title":"Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography","volume":"943","author":"Li","year":"2002","journal-title":"J. Chromatogr. A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.jchromb.2009.02.013","article-title":"Isolation and purification of salvianolic acid A and salvianolic acid B from Salvia miltiorrhiza by high-speed counter-current chromatography and comparison of their antioxidant activity","volume":"877","author":"Sun","year":"2009","journal-title":"J. Chromatogr. B"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2174\/0929867053363397","article-title":"Chemistry and biological activities of caffeic acid derivatives from Salvia miltiorrhiza","volume":"12","author":"Jiang","year":"2005","journal-title":"Curr. Med. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1749-8546-5-22","article-title":"Pharmacological effects of Salvia miltiorrhiza (Danshen) on cerebral infarction","volume":"5","author":"Lin","year":"2010","journal-title":"Chin. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jep.2005.09.040","article-title":"Traditional Chinese medicine Bao Gan Ning increase phosphorylation of CREB in liver fibrosis in vivo and in vitro","volume":"105","author":"Tan","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.fitote.2013.08.009","article-title":"Diastereomers of lithospermic acid and lithospermic acid B from Monarda fistulosa and Lithospermum erythrorhizon","volume":"91","author":"Murata","year":"2013","journal-title":"Fitoterapia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/j.microc.2019.02.049","article-title":"Monoamine oxidase B immobilized on magnetic nanoparticles for screening of the enzyme\u2019s inhibitors from herbal extracts","volume":"146","author":"Jiang","year":"2019","journal-title":"Microchem. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.micromeso.2007.05.052","article-title":"Covalent-bonded immobilization of enzyme on hydrophilic polymer covering magnetic nanogels","volume":"109","author":"Hong","year":"2008","journal-title":"Micropor. Mesopor. Mat."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3358\/shokueishi.31.233","article-title":"Studies on anti-allergic activity in tea. II. Inhibitory effect of tea extracts on hyaluronidase","volume":"31","author":"Maeda","year":"1990","journal-title":"J. Food Hyg. Soc. Jpn."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1021\/acs.jafc.5b05320","article-title":"Anti-hyaluronidase Activity in Vitro and Amelioration of Mouse Experimental Dermatitis by Tomato Saponin, Esculeoside A","volume":"64","author":"Zhou","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zhou, J.R., Kitahara, N., Nakamura, H., Ono, T., Karashima, R., Fang, J., Nohara, T., and Yokomizo, K. (2022). Decrease of Hyaluronidase Activity and Suppression of Mouse CD4+ T Lymphocyte Activation by Tomato Juice Saponin Esculeoside B, and Its Sapogenol Esculeogenin B. J. Pers. Med., 12.","DOI":"10.3390\/jpm12040579"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1124\/pr.58.3.10","article-title":"Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies","volume":"58","author":"Chou","year":"2006","journal-title":"Pharmacol. Rev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.synres.2016.06.001","article-title":"Drug combination in vivo using combination index method: Taxotere and T607 against colon carcinoma HCT-116 xenograft tumor in nude mice","volume":"3","author":"Fu","year":"2016","journal-title":"Synergy"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"131278","DOI":"10.1016\/j.foodchem.2021.131278","article-title":"Caffeoyl substitution decreased the binding and inhibitory activity of quinic acid against alpha-amylase: The reason why chlorogenic acid is a relatively weak enzyme inhibitor","volume":"371","author":"Song","year":"2022","journal-title":"Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"623","DOI":"10.6026\/97320630017623","article-title":"Molecular docking analysis of COX-2 with compounds from piper longum","volume":"17","author":"Tripathi","year":"2021","journal-title":"Bioinformation"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"103825","DOI":"10.1016\/j.jff.2020.103825","article-title":"Isolation and identification of four antioxidants from Rhodiola crenulata and evaluation of their UV photoprotection capacity in vitro","volume":"66","author":"Wang","year":"2020","journal-title":"J. Funct. Foods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/7369\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:10:30Z","timestamp":1760109030000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/13\/7369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,5]]},"references-count":42,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ijms25137369"],"URL":"https:\/\/doi.org\/10.3390\/ijms25137369","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,5]]}}}