{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:15:26Z","timestamp":1760145326542,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T00:00:00Z","timestamp":1720224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Recovery and Resilience Facility of the European Union","award":["RRF-2.3.1-21-2022-00012"],"award-info":[{"award-number":["RRF-2.3.1-21-2022-00012"]}]},{"name":"HUN-REN Hungarian Research Network","award":["RRF-2.3.1-21-2022-00012"],"award-info":[{"award-number":["RRF-2.3.1-21-2022-00012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The receptive phase of the uterus is marked by structural and functional maturation of the endometrium. During this limited time span, the blastocyst competency is superimposed on the receptive endometrium. It is a well-known fact that lipid signalling in early-stage pregnancy has a crucial role in successful embryogenesis. In our study, CD-1 mouse uteri after normal and in vitro fertilization (IVF) were investigated at 6.5, 8.5, and 10.5 days of pregnancy. Matrix-assisted laser desorption\/ionization time-of-flight imaging mass spectrometry and liquid chromatography coupled tandem mass spectrometry were used for identification of phosphatidylcholine (PC) lipid structures. In the embryonal tissues, PC 32:0 and PC 34:0 were increased, while in the antemesometrial (AM) decidua the two 20:4-containing PCs, PC 36:4 and PC 38:4 were increased. In transferred uterus samples, higher expressions of PC 34:0, PC 34:1, PC 34:2, PC 36:1, and PC 36:2 in mesometrial decidua were seen, whereas the two 20:4-containing PCs, PC 36:4 and PC 38:4 showed increased expression in the AM and lateral decidua. This paper shows a significant spatio-temporal change in lipid metabolism during IVF procedures for the first time.<\/jats:p>","DOI":"10.3390\/ijms25137423","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T12:21:03Z","timestamp":1720441263000},"page":"7423","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of Phosphatidylcholine by MALDI Imaging Mass Spectrometry in Normal and IVF Early-Stage Embryos"],"prefix":"10.3390","volume":"25","author":[{"given":"Stef\u00e1nia","family":"Gitta","sequence":"first","affiliation":[{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3563-2492","authenticated-orcid":false,"given":"\u00c9va","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Sulc","sequence":"additional","affiliation":[{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e9ter","family":"Cz\u00e9t\u00e1ny","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"},{"name":"Urology Clinic, Clinical Center, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"M\u00e1t\u00e9","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"},{"name":"Pannon Reproduction Institute, 8300 Tapolca, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e1s","family":"Ball\u00f3","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"},{"name":"Urology Clinic, Clinical Center, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00edmea","family":"Csabai","sequence":"additional","affiliation":[{"name":"Institute of Biology, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1rp\u00e1d","family":"Sz\u00e1nt\u00f3","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"},{"name":"Urology Clinic, Clinical Center, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"M\u00e1rk","sequence":"additional","affiliation":[{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"},{"name":"Imaging Centre for Life and Material Sciences, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"HUN-REN-PTE, Human Reproduction Research Group, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.clinbiochem.2018.03.012","article-title":"Fertility and infertility: Definition and epidemiology","volume":"62","author":"Wyns","year":"2018","journal-title":"Clin. 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