{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:35:00Z","timestamp":1777566900472,"version":"3.51.4"},"reference-count":22,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pioneer and Leading Goose R&amp;D Program of Zhejiang","award":["2023C02047"],"award-info":[{"award-number":["2023C02047"]}]},{"name":"Pioneer and Leading Goose R&amp;D Program of Zhejiang","award":["2020C02032"],"award-info":[{"award-number":["2020C02032"]}]},{"name":"Zhejiang Provincial Key R&amp;D Program of China","award":["2023C02047"],"award-info":[{"award-number":["2023C02047"]}]},{"name":"Zhejiang Provincial Key R&amp;D Program of China","award":["2020C02032"],"award-info":[{"award-number":["2020C02032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The synergetic strategy has created tremendous advantages in drug-resistance bacterial infection treatment, whereas challenges related to novel compound discovery and identifying drug-binding targets still remain. The mechanisms of antimicrobial resistance involving \u03b2-lactamase catalysis and the degradation of \u03b2-lactam antibiotics are being revealed, with relevant therapies promising to improve the efficacy of existing major classes of antibiotics in the foreseeable future. In this study, it is demonstrated that nordalbergin, a coumarin isolated from the wood bark of Dalbergia sissoo, efficiently potentiated the activities of \u03b2-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA) by suppressing \u03b2-lactamase performance and improving the bacterial biofilm susceptibility to antibiotics. Nordalbergin was found to destabilize the cell membrane and promote its permeabilization. Moreover, nordalbergin efficiently improved the therapeutic efficacy of amoxicillin against MRSA pneumonia in mice, as supported by the lower bacterial load, attenuated pathological damage, and decreased inflammation level. These results demonstrate that nordalbergin might be a promising synergist of amoxicillin against MRSA infections. This study provided a new approach for developing potentiators for \u03b2-lactam antibiotics against MRSA infections.<\/jats:p>","DOI":"10.3390\/ijms25147704","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T15:17:06Z","timestamp":1721056626000},"page":"7704","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nordalbergin Synergizes with Novel \u03b2-Lactam Antibiotics against MRSA Infection"],"prefix":"10.3390","volume":"25","author":[{"given":"Haiting","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangyu","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China"},{"name":"Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzhu","family":"Pei","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China"},{"name":"Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4849-7127","authenticated-orcid":false,"given":"Hongxiang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Harkins, C.P., Pichon, B., Doumith, M., Parkhill, J., Westh, H., Tomasz, A., De Lencastre, H., Bentley, S.D., Kearns, A.M., and Holden, M.T.G. 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