{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T19:27:31Z","timestamp":1784834851938,"version":"3.55.0"},"reference-count":42,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["2022R1G1A1008848"],"award-info":[{"award-number":["2022R1G1A1008848"]}]},{"name":"Korean government (MSIT)","award":["2022R1G1A1008848"],"award-info":[{"award-number":["2022R1G1A1008848"]}]},{"name":"EVERBIO","award":["2022R1G1A1008848"],"award-info":[{"award-number":["2022R1G1A1008848"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Androgenetic alopecia is a genetic disorder that commonly causes progressive hair loss in men, leading to diminished self-esteem. Although cannabinoids extracted from Cannabis sativa are used in hair loss treatments, no study has evaluated the effects of germinated hemp seed extract (GHSE) and exosomes derived from the calli of germinated hemp seeds on alopecia. Therefore, this study aimed to demonstrate their preventive effects against alopecia using various methodologies, including quantitative PCR, flow cytometry, ELISA, and immunocytochemistry. Our research highlights the preventive functions of GHSE (GE2000: 2000 \u00b5g\/mL) and exosomes from the calli of germinated hemp seeds (E40: 40 \u03bcg\/mL) in three biochemical categories: genetic modulation in hair follicle dermal papilla stem cells (HFDPSCs), cellular differentiation, and immune system modulation. Upon exposure to dihydrotestosterone (DT), both biomaterials upregulated genes preventing alopecia (Wnt, \u03b2-catenin, and TCF) in HFDPSCs and suppressed genes activating alopecia (STAT1, 5\u03b1-reductase type 1, IL-15R). Additionally, they suppressed alopecia-related genes (NKG2DL, IL2-R\u03b2, JAK1, STAT1) in CD8+ T cells. Notably, E40 exhibited more pronounced effects compared to GE2000. Consequently, both E40 and GE2000 effectively mitigated DT-induced stress, activating mechanisms promoting hair formation. Given the limited research on alopecia using these materials, their pharmaceutical development promises significant economic and health benefits.<\/jats:p>","DOI":"10.3390\/ijms25147823","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T16:35:58Z","timestamp":1721234158000},"page":"7823","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Biomaterials Derived from Germinated Hemp Seeds on Stressed Hair Stem Cells and Immune Cells"],"prefix":"10.3390","volume":"25","author":[{"given":"Donghyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, Andong National University, Andong 36729, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-1084","authenticated-orcid":false,"given":"Namsoo Peter","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Cannabis Certificate, Makers\u2019 Station, Washington, DC 20001, USA"},{"name":"Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4029-3824","authenticated-orcid":false,"given":"Boyong","family":"Kim","sequence":"additional","affiliation":[{"name":"EVERBIO, 131, Jukhyeon-gil, Gwanghyewon-myeon, Jincheon-gun 27809, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.15406\/jdc.2021.05.00190","article-title":"Alopecia types, current and future treatment","volume":"5","author":"Rambwawasvika","year":"2021","journal-title":"J. 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