{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:23:11Z","timestamp":1780496591286,"version":"3.54.1"},"reference-count":33,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:00Z","timestamp":1721260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tianjin Science and Technology Commission","award":["22JCQNJC00150"],"award-info":[{"award-number":["22JCQNJC00150"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Epinephrine (EP) is a very important chemical transmitter in the transmission of nerve impulses in the central nervous system of mammals. Ascorbic acid (AA) is considered to be the most important extracellular fluid antioxidant and has important antioxidant properties in the cell. In this study, a series of transition metal\u2013polyhistidine-carboxylated multi-wall carbon nanotube nanocomposites were synthesized, and their simultaneous catalytic effects on epinephrine and ascorbic acid were investigated. The results showed that nanocomposites based on iron ions had the highest catalytic activity. The prepared biosensor expressed high selectivity toward EP and AA with LOD values of 0.1 \u03bc\u039c (AA) and 0.01 \u03bc\u039c (EP), and sensitivity values of 4.18 \u03bcA mM\u22121 with a range of 0.001\u20135 mM (AA), 50.98 \u03bcA mM\u22121 with a range of 0.2\u2013100 \u03bcM (EP), and 265.75 \u03bcA mM\u22121 with a range of 0.1\u20131.0 mM (EP). Moreover, it showed good stability, good repeatability and high selectivity in real sample detection. This work is a reference for the design of new electrochemical enzyme-free biosensors and the detection of biomarkers.<\/jats:p>","DOI":"10.3390\/ijms25147883","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T14:59:50Z","timestamp":1721314790000},"page":"7883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Biosensor for Simultaneous Detection of Epinephrine and Ascorbic Acid Based on Fe(III)\u2013Polyhistidine-Functionalized Multi-Wall Carbon Nanotube Composites"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7120-5973","authenticated-orcid":false,"given":"Bingkai","family":"Han","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, No. 16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin 301617, China"},{"name":"The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Chen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science, Henan University, No. 379 Mingli Road, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilong","family":"Yan","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, No. 16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin 301617, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, No. 16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin 301617, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziru","family":"Huang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, No. 16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin 301617, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, No. 16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin 301617, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"AB2","DOI":"10.1016\/j.jaci.2021.12.050","article-title":"Subanalysis of an Open-Label, Crossover Study to Assess the Relative Bioavailability of Self-administered Nasal Epinephrine Compared to Administration by Trained Health Personnel in Healthy Adult Subjects","volume":"149","author":"Dworaczyk","year":"2022","journal-title":"J. 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