{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:29:04Z","timestamp":1790753344699,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T00:00:00Z","timestamp":1721520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Previous studies have revealed the medicinal and therapeutic effects of Galla chinensis. However, no studies have focused on the antihypertensive effects of G. chinensis. Therefore, we aimed to determine the vasorelaxant and hypotensive effects of G. chinensis 50% ethanolic extract (GCE). To evaluate the vascular relaxing effect of GCE, experiments were conducted using aortic segments dissected from Sprague Dawley rats. GCE showed a vasorelaxant effect via the nitric oxide\/cyclic guanosine 3\u2032,5\u2032-monophosphate pathway, inhibiting Ca2+ channels, and activating K+ channels. The hypotensive effects of GCE were evaluated in spontaneously hypertensive rats (SHRs). The SHRs were randomly divided into a control group and orally administered GCE group (100 or 300 mg\/kg). The systolic and diastolic blood pressure decreased significantly by \u221219.47 \u00b1 4.58% and \u221231.14 \u00b1 7.66% in the GCE 100 mg\/kg group, and \u221221.64 \u00b1 2.40% and \u221231.91 \u00b1 5.75% in the GCE 300 mg\/kg group at 4 h after administration. Considering its vasorelaxant and hypotensive effects, our results indicate that GCE may be a valuable solution for the control of hypertension. However, further studies on the long-term administration and toxicity of GCE are required.<\/jats:p>","DOI":"10.3390\/ijms25147962","type":"journal-article","created":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T07:57:23Z","timestamp":1721721443000},"page":"7962","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vasorelaxant and Hypotensive Effects of Galla chinensis in Rats"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5583-473X","authenticated-orcid":false,"given":"Sujin","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5958-4944","authenticated-orcid":false,"given":"Junkyu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0345-3000","authenticated-orcid":false,"given":"Ho-Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngmin","family":"Bu","sequence":"additional","affiliation":[{"name":"Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-9480","authenticated-orcid":false,"given":"Kyungjin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"0963689720920830","DOI":"10.1177\/0963689720920830","article-title":"Cardiovascular Changes Associated with Hypertensive Heart Disease and Aging","volume":"29","author":"Saheera","year":"2020","journal-title":"Cell Transplant."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E558","DOI":"10.1161\/CIR.0000000000000570","article-title":"Projected Costs of Informal Caregiving for Cardiovascular Disease: 2015 to 2035 A Policy Statement from the American Heart Association","volume":"137","author":"Dunbar","year":"2018","journal-title":"Circulation"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1016\/j.jacc.2020.04.038","article-title":"Association of Age of Onset of Hypertension with Cardiovascular Diseases and Mortality","volume":"75","author":"Wang","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_4","unstructured":"NCD Risk Factor Collaboration (NCD-RisC) (2017). Worldwide trends in blood pressure from 1975 to 2015: A pooled analysis of 1479 population-based measurement studies with 19.1 million participants. Lancet, 389, 37\u201355."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1097\/HJH.0000000000002294","article-title":"Medication adherence in hypertension","volume":"38","author":"Poulter","year":"2020","journal-title":"J. Hypertens."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Budreviciute, A., Damiati, S., Sabir, D.K., Onder, K., Schuller-Goetzburg, P., Plakys, G., Katileviciute, A., Khoja, S., and Kodzius, R. (2020). Management and Prevention Strategies for Non-communicable Diseases (NCDs) and Their Risk Factors. Front. Public Health, 8.","DOI":"10.3389\/fpubh.2020.574111"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Yuan, H., Ma, Q., Ye, L., and Piao, G. (2016). The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21.","DOI":"10.3390\/molecules21050559"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5430407","DOI":"10.1155\/2020\/5430407","article-title":"Natural Drugs as a Treatment Strategy for Cardiovascular Disease through the Regulation of Oxidative Stress","volume":"2020","author":"Chang","year":"2020","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"624","DOI":"10.2174\/0113816128295053240207090928","article-title":"The Potential of Natural Products in the Management of Cardiovascular Disease","volume":"30","author":"Singhai","year":"2024","journal-title":"Curr. Pharm. Design"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1039\/c3np20120a","article-title":"21st Century natural product research and drug development and traditional medicines","volume":"30","author":"Ngo","year":"2013","journal-title":"Nat. Prod. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"114247","DOI":"10.1016\/j.jep.2021.114247","article-title":"Galla Chinensis, a Traditional Chinese Medicine: Comprehensive review of botany, traditional uses, chemical composition, pharmacology and toxicology","volume":"278","author":"Ren","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1002\/ptr.3215","article-title":"Rhus chinensis and Galla Chinensis\u2014Folklore to Modern Evidence: Review","volume":"24","author":"Djakpo","year":"2010","journal-title":"Phytother. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4930371","DOI":"10.1155\/2018\/4930371","article-title":"Gallnuts: A Potential Treasure in Anticancer Drug Discovery","volume":"2018","author":"Gao","year":"2018","journal-title":"Evid-Based Compl Alt."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Shin, S., Park, J., Choi, H.-Y., Bu, Y., and Lee, K. (2024). Blood-Pressure-Lowering and Endothelium-Dependent Vasorelaxant Effects of Nutgall Tree in Rats. Foods, 13.","DOI":"10.3390\/foods13071041"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Cabou, C., and Martinez, L.O. (2022). The Interplay of Endothelial P2Y Receptors in Cardiovascular Health: From Vascular Physiology to Pathology. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23115883"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sheikh, A.M., Yano, S., Tabassum, S., and Nagai, A. (2024). The Role of the Vascular System in Degenerative Diseases: Mechanisms and Implications. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25042169"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/labinvest.3700215","article-title":"The role of shear stress in the pathogenesis of atherosclerosis","volume":"85","author":"Cunningham","year":"2005","journal-title":"Lab. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1080\/10408363.2017.1394267","article-title":"The vascular endothelium: A regulator of arterial tone and interface for the immune system","volume":"54","author":"Mallat","year":"2017","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Othman, Z.A., Zakaria, Z., Suleiman, J.B., Nna, V.U., Romli, A.C., Ghazali, W.S.W., and Mohamed, M. (2021). Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS\/NO\/cGMP-Signaling Pathway. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22084225"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"S27","DOI":"10.1097\/01.hjh.0000358834.18311.fc","article-title":"Endothelial dysfunction in spontaneously hypertensive rats: Focus on methodological aspects","volume":"27","author":"Bernatova","year":"2009","journal-title":"J. Hypertens."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ahmad, A., Dempsey, S.K., Daneva, Z., Azam, M., Li, N., Li, P.-L., and Ritter, J.K. (2018). Role of Nitric Oxide in the Cardiovascular and Renal Systems. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092605"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"E146","DOI":"10.1161\/ATVBAHA.119.312004","article-title":"Ion Channels and Vascular Diseases","volume":"39","author":"Cheng","year":"2019","journal-title":"Arterioscler. Thromb. Vasc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/cvr\/cvy023","article-title":"Vascular smooth muscle contraction in hypertension","volume":"114","author":"Touyz","year":"2018","journal-title":"Cardiovasc. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"51","DOI":"10.2147\/JRLCR.S36062","article-title":"K+ channels in biological processes: Vascular K+ channels in the regulation of blood pressure","volume":"7","author":"Werner","year":"2014","journal-title":"J. Recept. Ligand Channel Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"C799","DOI":"10.1152\/ajpcell.1995.268.4.C799","article-title":"Physiological roles and properties of potassium channels in arterial smooth muscle","volume":"268","author":"Nelson","year":"1995","journal-title":"Am. J. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1161\/CIRCRESAHA.116.303587","article-title":"Hypertension: Renin-Angiotensin-Aldosterone System Alterations","volume":"116","author":"Riet","year":"2015","journal-title":"Circ. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Tain, Y.-L., and Hsu, C.-N. (2024). The Renin-Angiotensin System and Cardiovascular-Kidney-Metabolic Syndrome: Focus on Early-Life Programming. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25063298"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Shin, S., Park, J., Choi, H.-Y., Bu, Y., and Lee, K. (2024). Sakuranetin as a Potential Regulator of Blood Pressure in Spontaneously Hypertensive Rats by Promoting Vasorelaxation through Calcium Channel Blockade. Biomedicines, 12.","DOI":"10.3390\/biomedicines12020346"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Park, J., Shin, S., Bu, Y., Choi, H.-Y., and Lee, K. (2024). Vasorelaxant and Blood Pressure-Lowering Effects of Cnidium monnieri Fruit Ethanol Extract in Sprague Dawley and Spontaneously Hypertensive Rats. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25084223"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0008-6363(98)00076-5","article-title":"Rat models of hypertension, cardiac hypertrophy and failure","volume":"39","author":"Doggrell","year":"1998","journal-title":"Cardiovasc. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1161\/01.HYP.7.4.483","article-title":"Aortic endothelial junctions in developing hypertension","volume":"7","author":"McGuire","year":"1985","journal-title":"Hypertension"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1161\/01.HYP.35.1.480","article-title":"Gender differences in development of hypertension in spontaneously hypertensive rats: Role of the renin-angiotensin system","volume":"35","author":"Reckelhoff","year":"2000","journal-title":"Hypertension"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1042\/CS20201017","article-title":"Sex differences in hypertension: Lessons from spontaneously hypertensive rats (SHR)","volume":"135","author":"Elmarakby","year":"2021","journal-title":"Clin. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jep.2014.12.021","article-title":"Acute and subchronic toxicity as well as evaluation of safety pharmacology of Galla chinensis solution","volume":"162","author":"Xiang","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Jung, J., Shin, S., Park, J., Lee, K., and Choi, H.-Y. (2023). Hypotensive and Vasorelaxant Effects of Sanguisorbae Radix Ethanol Extract in Spontaneously Hypertensive and Sprague Dawley Rats. Nutrients, 15.","DOI":"10.3390\/nu15214510"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/14\/7962\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:20:40Z","timestamp":1760109640000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/14\/7962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,21]]},"references-count":35,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["ijms25147962"],"URL":"https:\/\/doi.org\/10.3390\/ijms25147962","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,21]]}}}