{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:18:17Z","timestamp":1760145497447,"version":"build-2065373602"},"reference-count":94,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hungarian Brain Research Program NAP 3.0","award":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"],"award-info":[{"award-number":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"]}]},{"name":"Thematic Excellence Program","award":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"],"award-info":[{"award-number":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"]}]},{"name":"Medical School at University of P\u00e9cs","award":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"],"award-info":[{"award-number":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"]}]},{"name":"NKFIH","award":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"],"award-info":[{"award-number":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"]}]},{"name":"University of P\u00e9cs","award":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"],"award-info":[{"award-number":["TKP2021-EGA-16","HUN-REN TKI 14016","NKFIH K135457","KA-2024-10","KA-2024-07","TKP2021-EGA-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The behavior and presence of actin-regulating proteins are characteristic of various clinical diseases. Changes in these proteins significantly impact the cytoskeletal and regenerative processes underlying pathological changes. Pituitary adenylate cyclase-activating polypeptide (PACAP), a cytoprotective neuropeptide abundant in the nervous system and endocrine organs, plays a key role in neuron differentiation and migration by influencing actin. This study aims to elucidate the role of PACAP as an actin-regulating polypeptide, its effect on actin filament formation, and the underlying regulatory mechanisms. We examined PACAP27, PACAP38, and PACAP6-38, measuring their binding to actin monomers via fluorescence spectroscopy and steady-state anisotropy. Functional polymerization tests were used to track changes in fluorescent intensity over time. Unlike PACAP27, PACAP38 and PACAP6-38 significantly reduced the fluorescence emission of Alexa488-labeled actin monomers and increased their anisotropy, showing nearly identical dissociation equilibrium constants. PACAP27 showed weak binding to globular actin (G-actin), while PACAP38 and PACAP6-38 exhibited robust interactions. PACAP27 did not affect actin polymerization, but PACAP38 and PACAP6-38 accelerated actin incorporation kinetics. Fluorescence quenching experiments confirmed structural changes upon PACAP binding; however, all studied PACAP fragments exhibited the same effect. Our findings indicate that PACAP38 and PACAP6-38 strongly bind to G-actin and significantly influence actin polymerization. Further studies are needed to fully understand the biological significance of these interactions.<\/jats:p>","DOI":"10.3390\/ijms25158063","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T15:47:32Z","timestamp":1721836052000},"page":"8063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Role of Neuropeptide PACAP in Cytoskeletal Function Using Spectroscopic Methods"],"prefix":"10.3390","volume":"25","author":[{"given":"Roland G\u00e1bor","family":"V\u00e9kony","sequence":"first","affiliation":[{"name":"Department of Biophysics, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Tam\u00e1s","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e1s","family":"Luk\u00e1cs","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5936-7286","authenticated-orcid":false,"given":"Zolt\u00e1n","family":"Ujfalusi","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9nes","family":"L\u0151rinczy","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Tak\u00e1cs-Koll\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5201-1885","authenticated-orcid":false,"given":"P\u00e9ter","family":"Bukovics","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.2170\/jjphysiol.48.301","article-title":"Perspectives on Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) in the Neuroendocrine, Endocrine, and Nervous Systems","volume":"48","author":"Arimura","year":"1998","journal-title":"Jpn. 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