{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:29:46Z","timestamp":1784874586926,"version":"3.55.0"},"reference-count":30,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Recovery and Resilience Facility of the European Union","award":["RRF-2.3.1-21-2022-00012"],"award-info":[{"award-number":["RRF-2.3.1-21-2022-00012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Azoospermia, the absence of sperm cells in semen, affects around 15% of infertile males. Sertoli cell-only syndrome (SCOS) is the most common pathological lesion in the background of non-obstructive azoospermia and is characterised by the complete absence of germinal epithelium, with Sertoli cells exclusively present in the seminiferous tubules. Studies have shown a correlation between successful spermatogenesis and male fertility with lipid composition of spermatozoa, semen, seminal plasma or testis. The aim of this research was to discover the correlation between the Johnsen scoring system and phospholipid expressions in testicular cryosections of SCOS patients. MALDI imaging mass spectrometry is used to determine spatial distributions of molecular species, such as phospholipids. Phosphatidylcholines (PCs), phosphatidylethanolamines (PEs) and sphingomyelins (SMs) are the most abundant phospholipids in mammalian cells and testis. SMs, the structural components of plasma membranes, are crucial for spermatogenesis and sperm function. Plasmalogens, are unique PCs in testis with strong antioxidative properties. This study, using imaging mass spectrometry, demonstrates the local distribution of phospholipids, particularly SMs, PCs, plasmalogens and PEs in human testicular samples with SCOS for the first time. This study found a strong relationship between the Johnsen scoring system and phospholipid expression levels in human testicular tissues. Future findings could enable routine diagnostic techniques during microTESE procedures for successful sperm extraction.<\/jats:p>","DOI":"10.3390\/ijms25158358","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T08:02:27Z","timestamp":1722412947000},"page":"8358","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["MALDI Imaging Mass Spectrometry Reveals Lipid Alterations in Physiological and Sertoli Cell-Only Syndrome Human Testicular Tissue Sections"],"prefix":"10.3390","volume":"25","author":[{"given":"Alexandra","family":"Sulc","sequence":"first","affiliation":[{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7622 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"Cz\u00e9t\u00e1ny","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7622 P\u00e9cs, Hungary"},{"name":"Urology Clinic, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e1bor","family":"M\u00e1t\u00e9","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7622 P\u00e9cs, Hungary"},{"name":"Pannon Reproduction Institute, 8300 Tapolca, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e1s","family":"Ball\u00f3","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7622 P\u00e9cs, Hungary"},{"name":"Urology Clinic, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e1vid","family":"Semj\u00e9n","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1rp\u00e1d","family":"Sz\u00e1nt\u00f3","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7622 P\u00e9cs, Hungary"},{"name":"Urology Clinic, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"M\u00e1rk","sequence":"additional","affiliation":[{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7622 P\u00e9cs, Hungary"},{"name":"Imaging Centre for Life and Material Sciences, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"HUN-REN-PTE, Human Reproduction Research Group, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/humrep\/13.suppl_1.1","article-title":"Spermatogenesis","volume":"13","author":"Loveland","year":"1998","journal-title":"Hum. 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