{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:15:25Z","timestamp":1760112925213,"version":"build-2065373602"},"reference-count":100,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Scientists study the molecular activities of the hepatitis B virus (HBV). However, in vivo experiments are scarce. Some microRNAs are HBV-related, but their exact mechanisms are unknown. Our study provides an up-to-date view of the associations between microRNAs and HBV-DNA levels in chronically infected individuals. We conducted this large-scale research on five databases according to PRISMA guidance. Joanna Briggs Institute tools and Newcastle Ottawa Quality Assessment scores helped with quality evaluations. R 4.2.2 performed statistical computations for the meta-analysis. DIANA-microT 2023 and g:Profiler enriched the predictions of liver genes associated with miR-122 and miR-192-5p. From the 1313 records, we eliminated those irrelevant to our theme, non-article methodologies, non-English entries, and duplicates. We assessed associations between microRNAs and HBV-DNA levels. Overall, the pooled correlations favoured the general idea of the connection between non-coding molecules and viremia levels. MiR-122 and miR-192-5p were the most researched microRNAs, significantly associated with HBV-DNA levels. The connections between miR-122, miR-192-5p, let-7, miR-215, miR-320, and viral loads need further in vivo assessment. To conclude, this study evaluates systematically, for the first time, the correlations between non-coding molecules and viremia levels in patients. Our meta-analysis emphasizes potentially important pathways toward new inhibitors of the viral replication cycle.<\/jats:p>","DOI":"10.3390\/ijms25158410","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T12:38:20Z","timestamp":1722515900000},"page":"8410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B\u2014A Systematic Review and Meta-Analysis"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1164-5878","authenticated-orcid":false,"given":"Marina","family":"Manea","sequence":"first","affiliation":[{"name":"Immunology and Transplant Immunology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 020021 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4284-8654","authenticated-orcid":false,"given":"Ion","family":"M\u0103run\u021belu","sequence":"additional","affiliation":[{"name":"Immunology and Transplant Immunology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 020021 Bucharest, Romania"},{"name":"Centre of Immunogenetics and Virology, Fundeni Clinical Institute, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-2694","authenticated-orcid":false,"given":"Ileana","family":"Constantinescu","sequence":"additional","affiliation":[{"name":"Immunology and Transplant Immunology, University of Medicine and Pharmacy \u201cCarol Davila\u201d, 020021 Bucharest, Romania"},{"name":"Centre of Immunogenetics and Virology, Fundeni Clinical Institute, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,1]]},"reference":[{"key":"ref_1","unstructured":"European Centre for Disease Prevention and Control (2024, May 31). Prevention of Hepatitis B and C in the EU\/EEA, Available online: https:\/\/data.europa.eu\/doi\/10.2900\/703244."},{"key":"ref_2","unstructured":"Hepatitis, B. (2024, May 30). Surveillance Guidance, Available online: https:\/\/www.cdc.gov\/hepatitis\/statistics\/SurveillanceGuidance\/HepatitisB.htm."},{"key":"ref_3","unstructured":"Hepatitis, B. (2024, May 30). Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/hepatitis-b."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Torimiro, J.N.E., Duri, K., Goumkwa, N.M., Atah, S.M., Ondigui, J.-L.N., Lobe, C., Bouyou, M., Ndeboko, B., Moussa, A.M., and Police, C. (2024). Toward the elimination of hepatitis B: Networking to promote the prevention of vertical transmission of hepatitis B virus through population-based interventions and multidisciplinary groups in Africa. Front. Public Health, 12.","DOI":"10.3389\/fpubh.2024.1283350"},{"key":"ref_5","unstructured":"Nasrallah, G.K., Chemaitelly, H., Nizamuddin, P.B., Al-Sadeq, D.W., Shurrab, F.M., Amanullah, F.H., Al-Hamad, T.H., Mohammad, K.N., Alabdulmalek, M.A., and Al Kahlout, R.A. (2024). Prevalence of hepatitis B and C viruses among migrant workers in Qatar. Sci. Rep., 14."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zovich, B., Freeland, C., Moore, H., Sapp, K., Qureshi, A., Holbert, R., Zambrano, J., Bhangoo, D., Cohen, C., and Hass, R.W. (2024). Dismantling Barriers to Hepatitis B and Delta Screening, Prevention, and Linkage to Care among the PWUD Community in Philadelphia. Viruses, 16.","DOI":"10.20944\/preprints202404.0151.v1"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e080281","DOI":"10.1136\/bmjopen-2023-080281","article-title":"Prevalence of hepatitis B virus infection among general population of Armenia in 2021 and factors associated with it: A cross-sectional study","volume":"14","author":"Demirchyan","year":"2024","journal-title":"BMJ Open"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wu, D., Hu, Y., Wang, M., Wu, Y., Dong, J., Liu, J., and Hu, W. (2024). Establishing a screening strategy for non-discriminatory reactive blood donors by constructing a predictive model of hepatitis B virus infection status from a single blood center in China. Front. Public Health, 12.","DOI":"10.3389\/fpubh.2024.1366431"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5518","DOI":"10.12998\/wjcc.v10.i17.5518","article-title":"Occult hepatitis B-the result of the host immune response interaction with different genomic expressions of the virus","volume":"10","author":"Gherlan","year":"2022","journal-title":"World J. Clin. Cases"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kanda, T., Goto, T., Hirotsu, Y., Moriyama, M., and Omata, M. (2019). Molecular Mechanisms Driving Progression of Liver Cirrhosis towards Hepatocellular Carcinoma in Chronic Hepatitis B and C Infections: A Review. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20061358"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Xiong, Y., Wang, Z., Liu, J., Li, K., and Zhang, Y. (2024). The Effect of Low HBV-DNA Viral Load on Recurrence in Hepatocellular Carcinoma Patients Who Underwent Primary Locoregional Treatment and the Development of a Nomogram Prediction Model. Microorganisms, 12.","DOI":"10.3390\/microorganisms12050976"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zhang, D., Guo, S., and Schrodi, S.J. (2021). Mechanisms of DNA Methylation in Virus-Host Interaction in Hepatitis B Infection: Pathogenesis and Oncogenetic Properties. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22189858"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jss.2023.10.002","article-title":"Hepatitis B Virus DNA-Level Change is Associated with Tumor Recurrence in Patients with Resected Hepatitis B Virus Hepatocellular Carcinoma","volume":"295","author":"Cho","year":"2024","journal-title":"J. Surg. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s00705-024-06016-4","article-title":"Characterization of mutations in hepatitis B virus DNA isolated from Japanese HBsAg-positive blood donors in 2021 and 2022","volume":"169","author":"Sedohara","year":"2024","journal-title":"Arch. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3343\/alm.2023.0306","article-title":"Performance Evaluation of the Roche Cobas 5800 HBV and HCV Tests: Comparison of the 200 and 500 \u03bcL Protocols","volume":"44","author":"Jeong","year":"2023","journal-title":"Ann. Lab. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100956","DOI":"10.1016\/j.jhepr.2023.100956","article-title":"HBcrAg-based risk score performs better than the HBV DNA-based scores for HCC prediction in grey zone patients who are HBeAg-negative","volume":"6","author":"Tseng","year":"2024","journal-title":"JHEP Rep."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pietropaolo, V., Prezioso, C., and Moens, U. (2021). Role of Virus-Induced Host Cell Epigenetic Changes in Cancer. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22158346"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"78","DOI":"10.3390\/cimb45010006","article-title":"Liver Damage and microRNAs: An Update","volume":"45","author":"Cione","year":"2022","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Lauterbach-Rivi\u00e8re, L., Bergez, M., M\u00f6nch, S., Qu, B., Riess, M., Vondran, F.W.R., Liese, J., Hornung, V., Urban, S., and K\u00f6nig, R. (2020). Hepatitis B Virus DNA is a Substrate for the cGAS\/STING Pathway but is not Sensed in Infected Hepatocytes. Viruses, 12.","DOI":"10.3390\/v12060592"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kouwaki, T., Okamoto, M., Tsukamoto, H., Fukushima, Y., and Oshiumi, H. (2017). Extracellular Vesicles Deliver Host and Virus RNA and Regulate Innate Immune Response. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18030666"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.3390\/v5112690","article-title":"Roles of microRNAs in the Hepatitis B Virus Infection and Related Diseases","volume":"5","author":"Thirion","year":"2013","journal-title":"Viruses"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Yan, L.-R., Liu, A.-R., Jiang, L.-Y., and Wang, B.-G. (2022). Non-coding RNA and hepatitis B virus-related hepatocellular carcinoma: A bibliometric analysis and systematic review. Front. Med., 9.","DOI":"10.3389\/fmed.2022.995943"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1002\/hep.24809","article-title":"Loss of microRNA 122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G1-modulated P53 activity","volume":"55","author":"Wang","year":"2012","journal-title":"Hepatology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.omtn.2017.04.007","article-title":"Sustained Inhibition of HBV Replication In Vivo after Systemic Injection of AAVs Encoding Artificial Antiviral Primary MicroRNAs","volume":"7","author":"Maepa","year":"2017","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"n160","DOI":"10.1136\/bmj.n160","article-title":"PRISMA 2020 explanation and elaboration: Updated guidance and exemplars for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jclinepi.2020.01.008","article-title":"A full systematic review was completed in 2 weeks using automation tools: A case study","volume":"121","author":"Clark","year":"2020","journal-title":"J. Clin. Epidemiol."},{"key":"ref_27","unstructured":"(2023, February 01). ZOTERO. Available online: http:\/\/www.zotero.org."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Aromataris, E., and Munn, Z. (2020). Chapter 7: Systematic reviews of etiology and risk. JBI Manual for Evidence Synthesis, JBI. Available online: https:\/\/synthesismanual.jbi.global.","DOI":"10.46658\/JBIRM-17-01"},{"key":"ref_29","unstructured":"Wells, G.A., Shea, B., O\u2019Connell, D., Pereson, J., Welch, V., Losos, M., and Tugwell, P. (2024, June 29). The Newcastle-Ottawa Scale (NOS) for Assessing the Quality of Nonrandomized Studies in Meta-Analysis. Available online: https:\/\/www.ohri.ca\/programs\/clinical_epidemiology\/oxford.asp."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Modesti, P.A., Reboldi, G., Cappuccio, F.P., Agyemang, C., Remuzzi, G., Rapi, S., Perruolo, E., and Parati, G. (2016). Settings EWG on CR in LR. Panethnic Differences in Blood Pressure in Europe: A Systematic Review and Meta-Analysis. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0147601"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews Systematic reviews and Meta-Analyses","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"W148","DOI":"10.1093\/nar\/gkad283","article-title":"DIANA-microT 2023: Including predicted targets of virally encoded miRNAs","volume":"51","author":"Tastsoglou","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"W207","DOI":"10.1093\/nar\/gkad347","article-title":"g: Profiler\u2014interoperable web service for functional enrichment analysis and gene identifier mapping (2023 update)","volume":"51","author":"Kolberg","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_34","unstructured":"R Core Team (2022). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_35","unstructured":"Cooper, H., Hedges, L.V., and Valentine, J.C. (2009). The Handbook of Research Synthesis and Metaanalysis, Russell Sage Foundation. [2nd ed.]."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1136\/bmj.327.7414.557","article-title":"Measuring inconsistency in meta-analyses","volume":"327","author":"Higgins","year":"2003","journal-title":"BMJ"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/S0895-4356(01)00377-8","article-title":"Funnel plots for detecting bias in meta-analysis: Guidelines on choice of axis","volume":"54","author":"Sterne","year":"2001","journal-title":"J Clin Epidemiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1136\/bmj.315.7109.629","article-title":"Bias in meta-analysis detected by a simple, graphical test","volume":"315","author":"Egger","year":"1997","journal-title":"BMJ"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1002\/hep.24441","article-title":"MicroRNAs-372\/373 promote the expression of hepatitis B virus through the targeting of nuclear factor I\/B","volume":"54","author":"Guo","year":"2011","journal-title":"Hepatology"},{"key":"ref_40","first-page":"58","article-title":"Serum microRNA-122 levels in different groups of patients with chronic hepatitis B virus infection","volume":"19","author":"Waidmann","year":"2011","journal-title":"J. Viral Hepat."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1002\/jmv.23540","article-title":"Circulating microRNA-22 correlates with microRNA-122 and represents viral replication and liver injury in patients with chronic hepatitis B","volume":"85","author":"Arataki","year":"2013","journal-title":"J. Med. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Coppola, N., Potenza, N., Pisaturo, M., Mosca, N., Tonziello, G., Signoriello, G., Messina, V., Sagnelli, C., Russo, A., and Sagnelli, E. (2013). Liver microRNA hsa-miR-125a-5p in HBV Chronic Infection: Correlation with HBV Replication and Disease Progression. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065336"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.3892\/ol.2013.1638","article-title":"Circulating microRNA-101 as a potential biomarker for hepatitis B virus-related hepatocellular carcinoma","volume":"6","author":"Fu","year":"2013","journal-title":"Oncol. Lett."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Winther, T.N., Bang-Berthelsen, C.H., Heiberg, I.L., Pociot, F., and Hogh, B. (2013). Differential Plasma MicroRNA Profiles in HBeAg Positive and HBeAg Negative Children with Chronic Hepatitis B. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058236"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"110782","DOI":"10.1371\/journal.pone.0110782","article-title":"A Serum MicroRNA Signature Is Associated with the Immune Control of Chronic Hepatitis B Virus Infection","volume":"9","author":"Brunetto","year":"2014","journal-title":"PLoS ONE"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.4161\/cbt.29688","article-title":"Expression profiling of serum microRNA-101 in HBV-associated chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma","volume":"15","author":"Xie","year":"2014","journal-title":"Cancer Biol. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7912","DOI":"10.4238\/2014.September.29.4","article-title":"Expression and clinical significance of miR-122 and miR-29 in hepatitis B virus-related liver disease","volume":"13","author":"Xing","year":"2014","journal-title":"Genet. Mol. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jinf.2014.10.017","article-title":"Differences in serum microRNA profiles in hepatitis B and C virus infection","volume":"70","author":"Akamatsu","year":"2014","journal-title":"J. Infect."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Jin, B.-X., Zhang, Y.-H., Jin, W.-J., Sun, X.-Y., Qiao, G.-F., Wei, Y.-Y., Sun, L.-B., Zhang, W.-H., and Li, N. (2015). MicroRNA panels as disease biomarkers distinguishing hepatitis B virus infection caused hepatitis and liver cirrhosis. Sci. Rep., 5.","DOI":"10.1038\/srep15026"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"28315","DOI":"10.5812\/hepatmon.28315v2","article-title":"Negative Association of Plasma Levels of Vitamin D and mir-378 With Viral Load in Patients with Chronic Hepatitis B Infection","volume":"15","author":"Mohamadkhani","year":"2015","journal-title":"Hepat. Mon."},{"key":"ref_51","first-page":"5611","article-title":"Expression profile and clinical significance of miRNAs at different stages of chronic hepatitis B virus infection","volume":"8","author":"Xing","year":"2015","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"384.e1","DOI":"10.1016\/j.cmi.2015.12.024","article-title":"Serum microRNA-125b correlates with hepatitis B viral replication and liver necroinflammation","volume":"22","author":"Li","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"34483","DOI":"10.5812\/hepatmon.34483","article-title":"Expression of MicroRNA-155 is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Patients","volume":"16","author":"Yu","year":"2016","journal-title":"Hepat. Mon."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1007\/s13277-015-3841-0","article-title":"Low-level expression of microRNA-375 predicts poor prognosis in hepatocellular carcinoma","volume":"37","author":"Zhou","year":"2016","journal-title":"Tumor Biol."},{"key":"ref_55","first-page":"742","article-title":"Expression of microRNA-122 and microRNA-22 in HBV-related liver cancer and the correlation with clinical features","volume":"21","author":"Qiao","year":"2017","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"01919","DOI":"10.1128\/JVI.01919-16","article-title":"Hepatitis B Virus-Encoded MicroRNA Controls Viral Replication","volume":"91","author":"Yang","year":"2017","journal-title":"J. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1002\/jmv.25238","article-title":"Circulating microRNA-122 levels are important predictor of hepatitis B virus surface antigen seroclearance","volume":"90","author":"Akuta","year":"2018","journal-title":"J. Med. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1177\/0004563217741908","article-title":"Dysregulation of serum microRNA-574-3p and its clinical significance in hepatocellular carcinoma","volume":"55","author":"Shen","year":"2017","journal-title":"Ann. Clin. Biochem."},{"key":"ref_59","first-page":"1833","article-title":"HBV infection suppresses the expression of inflammatory macrophage miR-210","volume":"19","author":"Li","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"104355","DOI":"10.1016\/j.micpath.2020.104355","article-title":"The assessment of selected MiRNAs profile in HIV, HBV, HCV, HIV\/HCV, HIV\/HBV Co-infection and elite controllers for determination of biomarker","volume":"147","author":"Yousefpouran","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_61","first-page":"1028","article-title":"Correlation of serum microRNA-122 level with the levels of Alanine aminotransferase and HBV-DNA in Chronic HBV-infected patients","volume":"35","author":"Laleh","year":"2021","journal-title":"Med. J. Islam. Repub. Iran"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1093\/labmed\/lmaa110","article-title":"MicroRNA-138 Regulates T-Cell Function by Targeting PD-1 in Patients with Hepatitis B Virus-Related Liver Diseases","volume":"52","author":"Liu","year":"2021","journal-title":"Lab. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"536","DOI":"10.21037\/atm-22-2119","article-title":"The relationship between serum exosome HBV-miR-3 and current virological markers and its dynamics in chronic hepatitis B patients on antiviral treatment","volume":"10","author":"Gan","year":"2022","journal-title":"Ann. Transl. Med."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Loukachov, V., van Dort, K.A., Jansen, L., Reesink, H.W., and Kootstra, N.A. (2022). Identification of a Novel HBV Encoded miRNA Using Next Generation Sequencing. Viruses, 14.","DOI":"10.3390\/v14061223"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Loukachov, V., van Dort, K., Maurer, I., Takkenberg, R., de Niet, A., Reesink, H., Willemse, S., and Kootstra, N. (2022). Identification of Liver and Plasma microRNAs in Chronic Hepatitis B Virus infection. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.790964"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.cca.2015.08.022","article-title":"Serum microRNA-210 levels in different groups of chronic hepatitis B patients","volume":"450","author":"Loukachov","year":"2015","journal-title":"Clin. Chim. Acta"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1093\/infdis\/jix140","article-title":"Plasma MicroRNA Levels Are Associated with Hepatitis B e Antigen Status and Treatment Response in Chronic Hepatitis B Patients","volume":"215","author":"Jansen","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_68","first-page":"3805","article-title":"Baseline serum miR-125b levels predict virologic response to nucleos(t)ide analogue treatment in patients with HBeAg-positive chronic hepatitis B","volume":"16","author":"Zhou","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Liu, W., He, X., and Huang, F. (2021). Analysis of Serum MicroRNA-122 Expression at Different Stages of Chronic Hepatitis B Virus Infection. BioMed Res. Int., 2021.","DOI":"10.1155\/2021\/9957440"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ijid.2021.05.015","article-title":"Serum microRNAs predict response of patients with chronic hepatitis B to antiviral therapy","volume":"108","author":"Tan","year":"2021","journal-title":"Int. J. Infect. Dis."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1080\/22221751.2022.2037393","article-title":"Human hepatocyte-enriched miRNA-192-3p promotes HBV replication through inhibiting Akt\/mTOR signalling by targeting ZNF143 in hepatic cell lines","volume":"11","author":"Li","year":"2022","journal-title":"Emerg. Microbes Infect."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Nagura, Y., Matsuura, K., Iio, E., Fujita, K., Inoue, T., Matsumoto, A., Tanaka, E., Nishiguchi, S., Kang, J.-H., and Matsui, T. (2022). Serum miR-192-5p levels predict the efficacy of pegylated interferon therapy for chronic hepatitis B. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0263844"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"558","DOI":"10.4254\/wjh.v10.i9.558","article-title":"Micro-RNAs in hepatitis B virus-related chronic liver diseases and hepatocellular carcinoma","volume":"10","author":"Sagnelli","year":"2018","journal-title":"World J. Hepatol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3438","DOI":"10.4081\/ejh.2022.3438","article-title":"Long non-coding RNA WAC antisense RNA 1 mediates hepatitis B virus replication in vitro by reinforcing miR-192-5p\/ATG7-induced autophagy","volume":"66","author":"Cao","year":"2022","journal-title":"Eur. J. Histochem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"02009-17","DOI":"10.1128\/JVI.02009-17","article-title":"MicroRNA 130a Regulates both Hepatitis C Virus and Hepatitis B Virus Replication through a Central Metabolic Pathway","volume":"92","author":"Duan","year":"2018","journal-title":"J. Virol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"11031","DOI":"10.2147\/CMAR.S272983","article-title":"Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p\/TNFAIP3 Axis","volume":"12","author":"Jiang","year":"2020","journal-title":"Cancer Manag. Res."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Huang, J.-Y., Chen, H.-L., and Shih, C. (2016). MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms. Sci. Rep., 6.","DOI":"10.1038\/srep34740"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"4035","DOI":"10.2147\/DMSO.S373385","article-title":"ADAR1 Inhibits HBV DNA Replication via Regulating miR-122-5p in Palmitic Acid Treated HepG2.2.15 Cells","volume":"15","author":"Yang","year":"2022","journal-title":"Diabetes Metab. Syndr. Obes. Targets Ther."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Xu, J., An, P., Winkler, C.A., and Yu, Y. (2020). Dysregulated microRNAs in Hepatitis B Virus-Related Hepatocellular Carcinoma: Potential as Biomarkers and Therapeutic Targets. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.01271"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Karamichali, E., Foka, P., Papadopoulou, G., Loukaki-Gkountara, D., Andresaki, K., Koskinas, I., and Georgopoulou, U. (2022). Hepatitis Viruses Control Host Immune Responses by Modifying the Exosomal Biogenesis Pathway and Cargo. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810862"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Zheng, C., Su, H., Liu, M., Qian, Y., and Fan, H. (2024). miRNA-mRNA network contributes to HBV-related hepatocellular carcinoma via immune infiltration induced by GRB2. Biomed. Rep., 20.","DOI":"10.3892\/br.2024.1777"},{"key":"ref_82","first-page":"00376","article-title":"Differences in serum and plasma levels of microRNAs and their time-course changes after blood collection","volume":"39","author":"Wakabayashi","year":"2024","journal-title":"Pract. Lab. Med."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Wang, K., Yuan, Y., Cho, J.-H., McClarty, S., Baxter, D., and Galas, D.J. (2012). Comparing the MicroRNA Spectrum between Serum and Plasma. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0041561"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1261\/rna.1947110","article-title":"Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression","volume":"16","author":"Git","year":"2010","journal-title":"RNA"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Farr, R.J., Januszewski, A.S., Joglekar, M.V., Liang, H., McAulley, A.K., Hewitt, A.W., Thomas, H.E., Loudovaris, T., Kay, T.W.H., and Jenkins, A. (2015). A comparative analysis of high-throughput platforms for validation of a circulating microRNA signature in diabetic retinopathy. Sci. Rep., 5.","DOI":"10.1038\/srep10375"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Yen, Y.-H., Huang, C.-M., Wei, K.-L., Wang, J.-H., Lu, S.-N., Lee, C.-M., Hung, C.-H., Chen, C.-H., Tseng, P.-L., and Chang, K.-C. (2016). MicroRNA-122 as a predictor of HBsAg seroclearance in hepatitis B and C dual infected patients treated with interferon and ribavirin. Sci. Rep., 6.","DOI":"10.1038\/srep33816"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13027-016-0085-6","article-title":"Hepatitis B virus X protein mediated suppression of miRNA-122 expression enhances hepatoblastoma cell proliferation through cyclin G1-p53 axis","volume":"11","author":"Bandopadhyay","year":"2016","journal-title":"Infect. Agents Cancer"},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Spaniel, C., Honda, M., Selitsky, S.R., Yamane, D., Shimakami, T., Kaneko, S., Lanford, R.E., and Lemon, S.M. (2013). microRNA-122 Abundance in hepatocellular carcinoma and non-tumor liver tissue from Japanese patients with persistent HCV versus HBV infection. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0076867"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Koduru, S.V., Leberfinger, A.N., Kawasawa, Y.I., Mahajan, M., Gusani, N.J., Sanyal, A.J., and Ravnic, D.J. (2018). Non-coding RNAs in Various Stages of Liver Disease Leading to Hepatocellular Carcinoma: Differential Expression of miRNAs, piRNAs, lncRNAs, circRNAs, and sno\/mt-RNAs. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-26360-1"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Ma, L., Song, H., Zhang, C.-Y., and Hou, D. (2024). MiR-192-5p Ameliorates Hepatic Lipid Metabolism in Non-Alcoholic Fatty Liver Disease by Targeting Yy1. Biomolecules, 14.","DOI":"10.3390\/biom14010034"},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Jaeger, A., Zollinger, L., Saely, C.H., Muendlein, A., Evangelakos, I., Nasias, D., Charizopoulou, N., Schofield, J.D., Othman, A., and Soran, H. (2018). Circulating microRNAs-192 and-194 are associated with the presence and incidence of diabetes mellitus. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-32274-9"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1002\/ijc.29802","article-title":"Circulating microRNAs as biomarkers for diagnosis of early hepatocellular carcinoma associated with hepatitis B virus","volume":"138","author":"Hung","year":"2016","journal-title":"Int. J. Cancer"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Zulian, V., Fiscon, G., Paci, P., and Garbuglia, A.R. (2023). Hepatitis B Virus and microRNAs: A Bioinformatics Approach. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242417224"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1002\/iub.2408","article-title":"LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma","volume":"72","author":"Kong","year":"2020","journal-title":"IUBMB Life"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Li, G., Wang, Z., Chen, D., Yin, J., Mo, Z., Sun, B., Yang, T., Zhang, X., Zhai, Z., and Li, Y. (2022). Comprehensive analysis of a TPX2-related TRHDE-AS1\/PKIA ceRNA network involving prognostic signatures in Hepatitis B virus-infected hepatocellular carcinoma. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.1025900"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Qin, J., Zhang, H., Geng, Y., and Ji, Q. (2020). How Kinesin-1 Utilize the Energy of Nucleotide: The Conformational Changes and Mechanochemical Coupling in the Unidirectional Motion of Kinesin-1. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21186977"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Krylova, S.V., and Feng, D. (2023). The Machinery of Exosomes: Biogenesis, Release, and Uptake. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24021337"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Peng, Y., Yang, Y., Li, Y., Shi, T., Luan, Y., and Yin, C. (2023). Exosome and virus infection. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1154217"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/j.ajpath.2013.07.007","article-title":"Comparative Evaluation of miRNA Expression between in Vitro and in Vivo Airway Epithelium Demonstrates Widespread Differences","volume":"183","author":"Chen","year":"2013","journal-title":"Am. J. Pathol."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Sneller, L., Lin, C., Price, A., Kottilil, S., and Chua, J.V. (2024). RNA Interference Therapeutics for Chronic Hepatitis B: Progress, Challenges, and Future Prospects. Microorganisms, 12.","DOI":"10.3390\/microorganisms12030599"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/15\/8410\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:28:11Z","timestamp":1760110091000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/15\/8410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,1]]},"references-count":100,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["ijms25158410"],"URL":"https:\/\/doi.org\/10.3390\/ijms25158410","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,8,1]]}}}