{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:18:59Z","timestamp":1760145539756,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"],"award-info":[{"award-number":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"]}]},{"name":"National Key R &amp; D Program of China","award":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"],"award-info":[{"award-number":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"]}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"],"award-info":[{"award-number":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Innovation Foundation of Fujian Agriculture and Forestry University","award":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"],"award-info":[{"award-number":["32371894","U1905201","2021YFD1400900","2023J01068","KFB22037","KFB23045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>MaltAtt-1 is an antimicrobial peptide isolated from Monochamus alternatus with nematocidal activity against pine wood nematode. In this study, a eukaryotic expression system based on Komagataella\u00a0phaffii GS115 was established, and its secretory expression of MaltAtt-1 was realized. The basic properties and secondary and tertiary structures of the antimicrobial peptide MaltAtt-1 were identified by bioinformatics analysis. MaltAtt-1 is a hydrophilic stable protein, mainly composed of an \u03b1-helix (Hh), \u03b2-folds (Ee), and irregular curls (Cc). The optimal fermentation conditions for MaltAtt-1 were determined by a single-factor test and the Box\u2013Behnken response surface method, including an induction time of 72 h, induction temperature of 30 \u00b0C, culture medium of pH 7.6, methanol volume fraction of 2.0%, and an initial glycerol concentration of 1%. The stability of MaltAtt-1 indicated its resistant to UV irradiation and repeated freezing and thawing, but the antibacterial activity decreased significantly under the influence of high temperature and a strong acid and base, and it decreased significantly to 1.1 cm and 0.83 cm at pH 2.0 and pH 10.0, respectively. The corrected mortality of B. xylophilus achieved 71.94% in 3 h at a concentration of 300 mg\u00b7L\u22121 MaltAtt-1 exposure. The results provide a theoretical basis for the antimicrobial peptide MaltAtt-1 to become a new green and efficient nematicide.<\/jats:p>","DOI":"10.3390\/ijms25168555","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T08:07:59Z","timestamp":1722931679000},"page":"8555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization and Stability Assessment of Monochamus alternatus Antimicrobial Peptide MaltAtt-1 in Komagataella phaffii GS115 for the Control of Pine Wood Nematode"],"prefix":"10.3390","volume":"25","author":[{"given":"Di","family":"Jiang","sequence":"first","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuhuizi","family":"Xu","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeguang","family":"Wang","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yu","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuda","family":"Xu","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Chu","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Hu","sequence":"additional","affiliation":[{"name":"Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Malewski, T., Borowik, P., Olejarski, I., Berezovska, D., Dyshko, V., Behnke-Borowczyk, J., Pusz, W., Matic, S., and Oszako, T. (2023). Mycobiome of post-agricultural soils 20 years after application of organic substrates and planting of pine seedlings. Forests, 14.","DOI":"10.3390\/f14010036"},{"key":"ref_2","first-page":"385","article-title":"On the taxonomy and morphology of the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer 1934) Nickle 1970","volume":"13","author":"Nickle","year":"1981","journal-title":"J. Nematol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"109198","DOI":"10.1016\/j.ecolind.2022.109198","article-title":"Classification of pine wilt disease at different infection stages by diagnostic hyperspectral bands","volume":"142","author":"Li","year":"2022","journal-title":"Ecol. Indic."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1002\/1526-4998(200010)56:10<937::AID-PS213>3.0.CO;2-B","article-title":"Emamectin benzoate as a candidate for a trunk-injection agent against the pine wood nematode, Bursaphelenchus xylophilus","volume":"56","author":"Takai","year":"2000","journal-title":"Pest. Manag. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s10658-011-9924-x","article-title":"Pine wilt disease: A threat to European forestry","volume":"133","author":"Vicente","year":"2012","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s12600-012-0272-y","article-title":"Prevention of pine wilt disease induced by Bursaphelenchus xylophilus and Monochamus galloprovincialis by trunk injection of emamectin benzoate","volume":"41","author":"Sousa","year":"2013","journal-title":"Phytoparasitica"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zhao, B.G., Futai, K., Sutherland, J.R., and Takeuchi, Y. (2008). Recent advances in the integrated management of the pine wood nematode in China. Pine Wilt Disease, Springer.","DOI":"10.1007\/978-4-431-75655-2"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s13530-020-00068-0","article-title":"A short review of the pinewood nematode, Bursaphelenchus xylophilus","volume":"12","author":"Kim","year":"2020","journal-title":"Toxicol. Environ. Health Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ngegba, P.M., Cui, G., Khalid, M.Z., and Zhong, G. (2022). Use of botanical pesticides in agriculture as an alternative to synthetic pesticides. Agriculture, 12.","DOI":"10.3390\/agriculture12050600"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ayilara, M.S., Adeleke, B.S., Akinola, S.A., Fayose, C.A., Adeyemi, U.T., Gbadegesin, L.A., Omole, R.K., Johnson, R.M., Uthman, Q.O., and Babalola, O.O. (2023). Biopesticides as a promising alternative to synthetic pesticides: A case for microbial pesticides, phytopesticides, and nanobiopesticides. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1040901"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2320","DOI":"10.1016\/j.peptides.2007.09.017","article-title":"Decoralin, a novel linear cationic alpha-helical peptide from the venom of the solitary eumenine wasp Oreumenes decorates","volume":"28","author":"Konno","year":"2007","journal-title":"Peptides"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1093\/bib\/bbz043","article-title":"Characterization and identification of antimicrobial peptides with different functional activities","volume":"21","author":"Chung","year":"2019","journal-title":"Brief. Bioinform."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Li, X., Zuo, S., Wang, B., Zhang, K., and Wang, Y. (2022). Antimicrobial mechanisms and clinical application prospects of antimicrobial peptides. Molecules, 27.","DOI":"10.3390\/molecules27092675"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6566","DOI":"10.3390\/ijms12106566","article-title":"The potential of antimicrobial peptides as biocides","volume":"12","author":"Laverty","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1080\/07388551.2020.1796576","article-title":"Antimicrobial peptides as therapeutic agents: Opportunities and challenges","volume":"40","author":"Mahlapuu","year":"2020","journal-title":"Crit. Rev. BioTechnol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Soares, A.M.S., Alencar, L.M.R., Dias, L.P., Lima, R.C., Rocha Silva, C., Santos-Oliveira, R., Oliveira, J.T.A., Junior, L.M.C., and Souza, P.F.N. (2021). New insights into anthelmintic mechanisms of action of a synthetic peptide: An ultrastructural and nanomechanical approach. Polymers, 13.","DOI":"10.3390\/polym13142370"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Hawamda, A.I.M., Reichert, S., Ali, M.A., Nawaz, M.A., Austerlitz, T., Schekahn, P., Abbas, A., Tenhaken, R., and Bohlmann, H. (2022). Characterization of an Arabidopsis defensin-like gene conferring resistance against nematodes. Plants, 11.","DOI":"10.3390\/plants11030280"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chu, X., Jiang, D., Yu, L., Li, M., Wu, S.Q., Zhang, F.P., and Hu, X. (2023). Heterologous expression and bioactivity determination of Monochamus alternatus antibacterial peptide gene in Komagataella phaffii (Pichia pastoris). Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065421"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5867","DOI":"10.1002\/jcp.29583","article-title":"Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins","volume":"235","author":"Karbalaei","year":"2020","journal-title":"J. Cell Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Tachioka, M., Sugimoto, N., Nakamura, A., Sunagawa, N., Ishida, T., Uchiyama, T., Igarashi, K., and Samejima, M. (2016). Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris. Biotechnol. Biofuels., 9.","DOI":"10.1186\/s13068-016-0613-z"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s00253-008-1666-0","article-title":"Increasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization","volume":"81","author":"Zhao","year":"2008","journal-title":"Appl. Microbiol. Biot."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mao, R., Teng, D., Wang, X., Zhang, Y., Jiao, J., Cao, X., and Wang, J. (2015). Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33. BMC Microbiol., 15.","DOI":"10.1186\/s12866-015-0389-5"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1080\/10408398.2013.763765","article-title":"Nisin as a food preservative: Part 1: Physicochemical properties, antimicrobial activity, and main uses","volume":"56","author":"Gharsallaoui","year":"2016","journal-title":"Crit. Rev. Food Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1002\/1097-0010(200006)80:8<1143::AID-JSFA610>3.0.CO;2-C","article-title":"Physicochemical and enzymatic changes of cod muscle proteins subjected to different freeze\u2013thaw cycles","volume":"80","author":"Benjakul","year":"2000","journal-title":"J. Sci. Food Agric."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"966","DOI":"10.4014\/jmb.0901.018","article-title":"Optimization of the functional expression of Coprinus cinereus peroxidase in Pichia pastoris by varying the host and promoter","volume":"19","author":"Kim","year":"2009","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1021\/bp060012+","article-title":"Evaluation of Mut+ and MutS Pichia pastoris phenotypes for high level extracellular scFv expression under feedback control of the methanol concentration","volume":"22","author":"Pla","year":"2006","journal-title":"Biotechnol. Prog."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s10529-006-9220-6","article-title":"Optimization of an enthanol production medium in very high gravity fermentation","volume":"29","author":"Wang","year":"2007","journal-title":"Biotechnol. Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s11947-011-0633-4","article-title":"Antimicrobial activity of peptide P34 during thermal processing","volume":"6","author":"Utpott","year":"2013","journal-title":"Food Bioprocess. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1093\/jac\/dkt322","article-title":"De novo generation of short antimicrobial peptides with enhanced stability and cell specificity","volume":"69","author":"Kim","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s13568-016-0295-8","article-title":"Antimicrobial activity and stability of the D-amino acid substituted derivatives of antimicrobial peptide polybia-MPI","volume":"6","author":"Zhao","year":"2016","journal-title":"AMB Expr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/bip.20543","article-title":"Advances in antimicrobial peptide immunobiology","volume":"84","author":"Yount","year":"2006","journal-title":"Peptide Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/S0005-2736(03)00233-5","article-title":"The role of the abundant phenylalanines in the mode of action of the antimicrobial peptide clavanin","volume":"1615","author":"Demel","year":"2003","journal-title":"Bba-Biomembranes"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/S0014-5793(98)01238-1","article-title":"Parasin I, an antimicrobial peptide derived from histone H2A in the catfish, Parasilurus asotus","volume":"437","author":"Park","year":"1998","journal-title":"Febs Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1016\/j.ijbiomac.2020.08.067","article-title":"Mining, heterologous expression, purification and characterization of 14 novel bacteriocins from Lactobacillus rhamnosus LS-8","volume":"164","author":"Guo","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Li, Z.X., Cheng, Q., Guo, H.N., Zhang, R.J., and Si, D.Y. (2020). Expression of hybrid peptide EF-1 in Pichia pastoris, its purification, and antimicrobial characterization. Molecules, 25.","DOI":"10.3390\/molecules25235538"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/16\/8555\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:30:35Z","timestamp":1760110235000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/16\/8555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":35,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["ijms25168555"],"URL":"https:\/\/doi.org\/10.3390\/ijms25168555","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,8,6]]}}}