{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:48:37Z","timestamp":1782838117852,"version":"3.54.5"},"reference-count":52,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2021-1063"],"award-info":[{"award-number":["075-15-2021-1063"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The functioning of the human cornea heavily relies on the maintenance of its extracellular matrix (ECM) mechanical properties. Within this context, corneal stromal fibroblasts (CSFs) are essential, as they are responsible for remodeling the corneal ECM. In this study, we used a decellularized human amniotic membrane (dHAM) and a custom fibrillar collagen film (FCF) to explore the effects of fibrillar materials on human CSFs. Our findings indicate that substrates like FCF can enhance the early development of focal adhesions (FAs), leading to the activation and propagation of mechanotransduction signals. This is primarily achieved through FAK autophosphorylation and YAP1 nuclear translocation pathways. Remarkably, inhibiting FAK autophosphorylation negated the observed changes. Proteome analysis further confirmed the central role of FAs in mechanotransduction propagation in CSFs cultured on FCF. This analysis also highlighted complex signaling pathways, including chromatin epigenetic modifications, in response to fibrillar substrates. Overall, our research highlights the potential pathways through which CSFs undergo behavioral changes when exposed to fibrillar substrates, identifying FAs as essential mechanotransducers.<\/jats:p>","DOI":"10.3390\/ijms25168601","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T11:33:52Z","timestamp":1723030432000},"page":"8601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6270-2866","authenticated-orcid":false,"given":"Kirill E.","family":"Zhurenkov","sequence":"first","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"},{"name":"Department of Cytology and Histology, St. Petersburg State University, St. Petersburg 199032, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0930-1171","authenticated-orcid":false,"given":"Arseniy A.","family":"Lobov","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0921-5131","authenticated-orcid":false,"given":"Natalya B.","family":"Bildyug","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elga I.","family":"Alexander-Sinclair","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana M.","family":"Darvish","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3732-0553","authenticated-orcid":false,"given":"Ekaterina V.","family":"Lomert","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7147-1884","authenticated-orcid":false,"given":"Daria V.","family":"Kriger","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bozhana R.","family":"Zainullina","sequence":"additional","affiliation":[{"name":"Centre for Molecular and Cell Technologies, St. Petersburg State University, St. Petersburg 199032, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina S.","family":"Chabina","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia I.","family":"Khorolskaya","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5056-3368","authenticated-orcid":false,"given":"Daria A.","family":"Perepletchikova","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miralda I.","family":"Blinova","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia A.","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Chen, Z., You, J., Liu, X., Cooper, S., Hodge, C., Sutton, G., Crook, J.M., and Wallace, G.G. 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