{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T15:09:18Z","timestamp":1771513758997,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UANL-CA-112-Immunology academic group","award":["242078\/256477"],"award-info":[{"award-number":["242078\/256477"]}]},{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n and UAZ-CA-5-Autoimmunidad academic group","award":["242078\/256477"],"award-info":[{"award-number":["242078\/256477"]}]},{"name":"Universidad Aut\u00f3noma de Zacatecas, M\u00e9xico","award":["242078\/256477"],"award-info":[{"award-number":["242078\/256477"]}]},{"name":"CONACYT scholarship","award":["242078\/256477"],"award-info":[{"award-number":["242078\/256477"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pemphigus is an autoimmune disease that affects the skin and mucous membranes, induced by the deposition of pemphigus IgG, which mainly targets desmogleins 1 and 3 (Dsg1 and 3). This autoantibody causes steric interference between Dsg1 and 3 and the loss of cell adhesion, producing acantholysis. This molecule and its cellular effects are clinically reflected as intraepidermal blistering. Pemphigus vulgaris-IgG (PV-IgG) binding involves p38MAPK-signaling-dependent caspase-3 activation. The present work assessed the in vitro effect of PV-IgG on the adherence of HaCaT cells dependent on caspase-3. PV-IgG induced cell detachment and apoptotic changes, as demonstrated by annexin fluorescent assays. The effect of caspase-3 induced by PV-IgG was suppressed in cells pre-treated with caspase-3-shRNA, and normal IgG (N-IgG) as a control had no relevant effects on the aforementioned parameters. The results demonstrated that shRNA reduces caspase-3 expression, as measured via qRT-PCR and via Western blot and immunofluorescence, and increases cell adhesion. In conclusion, shRNA prevented in vitro cell detachment and the late effects of apoptosis induced by PV-IgG on HaCaT cells, furthering our understanding of the molecular role of caspase-3 cell adhesion dependence in pemphigus disease.<\/jats:p>","DOI":"10.3390\/ijms25168864","type":"journal-article","created":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T05:47:11Z","timestamp":1723700831000},"page":"8864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["shRNA-Targeting Caspase-3 Inhibits Cell Detachment Induced by Pemphigus Vulgaris Autoantibodies in HaCaT Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9488-3788","authenticated-orcid":false,"given":"Deyanira","family":"Pacheco-Tovar","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Biological Sciences, UACB, Universidad Aut\u00f3noma de Zacatecas, Av. de la Revoluci\u00f3n Mexicana s\/n, Colonia Tierra y Libertad, Guadalupe CP 98615, Zacatecas, Mexico"},{"name":"School of Chemistry Sciences, Universidad Aut\u00f3noma de Zacatecas, Campus Universitario Siglo XXI, Carretera Zacatecas-Guadalajara, Ejido \u201cLa Escondida\u201d, Zacatecas CP 98160, Zacatecas, Mexico"},{"name":"Department of Immunology and Virology, Faculty of Biological Sciences, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Pedro de Alba s\/n, Ciudad Universitaria, San Nicol\u00e1s de los Garza CP 64450, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda-Guadalupe","family":"Pacheco-Tovar","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Biological Sciences, UACB, Universidad Aut\u00f3noma de Zacatecas, Av. de la Revoluci\u00f3n Mexicana s\/n, Colonia Tierra y Libertad, Guadalupe CP 98615, Zacatecas, Mexico"},{"name":"School of Chemistry Sciences, Universidad Aut\u00f3noma de Zacatecas, Campus Universitario Siglo XXI, Carretera Zacatecas-Guadalajara, Ejido \u201cLa Escondida\u201d, Zacatecas CP 98160, Zacatecas, Mexico"},{"name":"Department of Immunology and Virology, Faculty of Biological Sciences, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Pedro de Alba s\/n, Ciudad Universitaria, San Nicol\u00e1s de los Garza CP 64450, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9495-7994","authenticated-orcid":false,"given":"Santiago","family":"Saavedra-Alonso","sequence":"additional","affiliation":[{"name":"Department of Immunology and Virology, Faculty of Biological Sciences, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Pedro de Alba s\/n, Ciudad Universitaria, San Nicol\u00e1s de los Garza CP 64450, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2986-0755","authenticated-orcid":false,"given":"Pablo","family":"Zapata-Benavides","sequence":"additional","affiliation":[{"name":"Department of Immunology and Virology, Faculty of Biological Sciences, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Pedro de Alba s\/n, Ciudad Universitaria, San Nicol\u00e1s de los Garza CP 64450, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-3316","authenticated-orcid":false,"given":"Felipe-de-Jes\u00fas","family":"Torres-del-Muro","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Biological Sciences, UACB, Universidad Aut\u00f3noma de Zacatecas, Av. de la Revoluci\u00f3n Mexicana s\/n, Colonia Tierra y Libertad, Guadalupe CP 98615, Zacatecas, Mexico"},{"name":"School of Chemistry Sciences, Universidad Aut\u00f3noma de Zacatecas, Campus Universitario Siglo XXI, Carretera Zacatecas-Guadalajara, Ejido \u201cLa Escondida\u201d, Zacatecas CP 98160, Zacatecas, Mexico"},{"name":"Department of Immunology and Virology, Faculty of Biological Sciences, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Pedro de Alba s\/n, Ciudad Universitaria, San Nicol\u00e1s de los Garza CP 64450, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9767-4834","authenticated-orcid":false,"given":"Juan-Jos\u00e9","family":"Bollain-y-Goytia","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Biological Sciences, UACB, Universidad Aut\u00f3noma de Zacatecas, Av. de la Revoluci\u00f3n Mexicana s\/n, Colonia Tierra y Libertad, Guadalupe CP 98615, Zacatecas, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Herrera-Esparza","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Biological Sciences, UACB, Universidad Aut\u00f3noma de Zacatecas, Av. de la Revoluci\u00f3n Mexicana s\/n, Colonia Tierra y Libertad, Guadalupe CP 98615, Zacatecas, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Rodr\u00edguez-Padilla","sequence":"additional","affiliation":[{"name":"Department of Immunology and Virology, Faculty of Biological Sciences, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Pedro de Alba s\/n, Ciudad Universitaria, San Nicol\u00e1s de los Garza CP 64450, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3403-9849","authenticated-orcid":false,"given":"Esperanza","family":"Avalos-D\u00edaz","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Biological Sciences, UACB, Universidad Aut\u00f3noma de Zacatecas, Av. de la Revoluci\u00f3n Mexicana s\/n, Colonia Tierra y Libertad, Guadalupe CP 98615, Zacatecas, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17026","DOI":"10.1038\/nrdp.2017.26","article-title":"Pemphigus","volume":"3","author":"Kasperkiewicz","year":"2017","journal-title":"Nat. Rev. Dis. 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