{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:41:07Z","timestamp":1768315267800,"version":"3.49.0"},"reference-count":24,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"HyTest Ltd","award":["current payment"],"award-info":[{"award-number":["current payment"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Immunodetection of cardiac isoforms of troponin I (cTnI) and troponin T (cTnT) in blood samples is widely used for the diagnosis of acute myocardial infarction. The cardiac troponin complex (ITC-complex), comprising cTnI, cTnT, and troponin C (TnC), makes up a large portion of troponins released into the bloodstream after the necrosis of cardiomyocytes. However, the stability of the ITC-complex has not been fully investigated. This study aimed to investigate the stability of the ITC-complex in blood samples. A native ITC-complex was incubated in buffer solutions, serum, and citrate, heparin,\u00a0or EDTA plasma at various temperatures. Western blotting and gel filtration were performed, and troponins were detected using specific monoclonal antibodies. The ITC-complex dissociated at 37 \u00b0C in buffers with or without anticoagulants, in citrate, heparin, and EDTA plasmas, and in serum, into a binary cTnI-TnC complex (IC-complex) and free cTnT. In plasma containing heparin and EDTA, the IC-complex further dissociated into free TnC and cTnI. No dissociation was found at 4 \u00b0C or at room temperature (RT) in all matrices within 24 h except for EDTA plasma. After incubation at 37 \u00b0C in EDTA plasma and serum, dissociation was accompanied by proteolytic degradation of both cTnI and cTnT. The presence of anti-troponin autoantibodies in the sample impeded dissociation of the ITC-complex. The ITC-complex dissociates in vitro to form the IC-complex and free cTnT at 37 \u00b0C but is mostly stable at 4 \u00b0C or RT. Further dissociation of the IC-complex occurs at 37 \u00b0C in plasmas containing heparin and EDTA.<\/jats:p>","DOI":"10.3390\/ijms25168919","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T06:23:20Z","timestamp":1723789400000},"page":"8919","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Influence of Anticoagulants on the Dissociation of Cardiac Troponin Complex in Blood Samples"],"prefix":"10.3390","volume":"25","author":[{"given":"Natalia S.","family":"Riabkova","sequence":"first","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"},{"name":"Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander E.","family":"Kogan","sequence":"additional","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"},{"name":"Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan A.","family":"Katrukha","sequence":"additional","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"},{"name":"Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1624-4141","authenticated-orcid":false,"given":"Alexandra V.","family":"Vylegzhanina","sequence":"additional","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnessa P.","family":"Bogomolova","sequence":"additional","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"},{"name":"Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amina K.","family":"Alieva","sequence":"additional","affiliation":[{"name":"National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, Akademik Chazov str., 15A, 121552 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5290-0065","authenticated-orcid":false,"given":"Dmitry V.","family":"Pevzner","sequence":"additional","affiliation":[{"name":"National Medical Research Centre of Cardiology Named after Academician E.I. Chazov, Akademik Chazov str., 15A, 121552 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia V.","family":"Bereznikova","sequence":"additional","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"},{"name":"Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey G.","family":"Katrukha","sequence":"additional","affiliation":[{"name":"HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland"},{"name":"Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1093\/labmed\/23.5.311","article-title":"Proteins of the Troponin Complex","volume":"23","author":"Katus","year":"1992","journal-title":"Lab. 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