{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:53:56Z","timestamp":1792724036530,"version":"4.5.0"},"reference-count":58,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Peripheral nerve injury (PNI) is a complex clinical challenge resulting in functional disability. Neurological recovery does not always ensure functional recovery, as extracellular matrix (ECM) alterations affect muscle function. This study evaluates hyaluronan (HA) and collagen concentration in the gastrocnemius muscle and thoracolumbar fascia (TLF) in unilateral lower limb PNI rats to explore systemic ECM alterations following PNI and their impacts on functional recovery. Eighteen 8-week-old male Sprague-Dawley rats were divided into experimental (n = 12 left sciatic nerve injury) and control (n = 6) groups. After six weeks, motor function was evaluated. Muscle and TLF samples were analysed for HA and collagen distribution and concentrations. SFI and gait analysis confirmed a functional deficit in PNI rats 6 weeks after surgery. HA concentration in both sides of the muscles decreased by approximately one-third; both sides showed significantly higher collagen concentration than healthy rats (12.74 \u00b1 4.83 \u00b5g\/g), with the left (32.92 \u00b1 11.34 \u00b5g\/g) significantly higher than the right (20.15 \u00b1 7.03 \u00b5g\/g). PNI rats also showed significantly lower HA (left: 66.95 \u00b1 20.08 \u00b5g\/g; right: 112.66 \u00b1 30.53 \u00b5g\/g) and higher collagen (left: 115.89 \u00b1 28.18 \u00b5g\/g; right: 90.43 \u00b1 20.83 \u00b5g\/g) concentrations in both TLF samples compared to healthy rats (HA: 167.18 \u00b1 31.13 \u00b5g\/g; collagen: 47.51 \u00b1 7.82 \u00b5g\/g), with the left TLF more affected. Unilateral lower limb PNI induced HA reduction and collagen accumulation in both the lower limb muscles and the TLF, potentially exacerbating motor function impairment and increasing the risk of low back dysfunctions.<\/jats:p>","DOI":"10.3390\/ijms25168945","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T12:40:07Z","timestamp":1723812007000},"page":"8945","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Impact of Sciatic Nerve Injury on Extracellular Matrix of Lower Limb Muscle and Thoracolumbar Fascia: An Observational Study"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1237-3689","authenticated-orcid":false,"given":"Xiaoxiao","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"},{"name":"Padova Neuroscience Center, University of Padova, 35129 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4372-7889","authenticated-orcid":false,"given":"Caterina","family":"Fede","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Petrelli","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0119-6549","authenticated-orcid":false,"given":"Carmelo","family":"Pirri","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Stocco","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"},{"name":"Department of Women\u2019s and Children\u2019s Health, University of Padova, 35128 Padova, Italy"},{"name":"Department of Surgery, Oncology and Gastroenterology, University of Padova, 35124 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglei","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Porzionato","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7493-563X","authenticated-orcid":false,"given":"Cesare","family":"Tiengo","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Unit, Department of Neuroscience, University of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2307-0277","authenticated-orcid":false,"given":"Raffaele","family":"De Caro","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0361-4898","authenticated-orcid":false,"given":"Stefano","family":"Masiero","sequence":"additional","affiliation":[{"name":"Neurorehabilitation Unit, Department of Neuroscience, General Hospital\u2014University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-4555","authenticated-orcid":false,"given":"Carla","family":"Stecco","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy, Department of Neurosciences, University of Padova, 35121 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/j.bjps.2008.06.041","article-title":"Clinical evaluation of a resorbable wrap-around implant as an alternative to nerve repair: A prospective, assessor-blinded, randomised clinical study of sensory, motor and functional recovery after peripheral nerve repair","volume":"62","author":"Aberg","year":"2009","journal-title":"J. Plast. Reconstr. Aesthet. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.hcl.2013.04.002","article-title":"Nerve physiology: Mechanisms of injury and recovery","volume":"29","author":"Menorca","year":"2013","journal-title":"Hand Clin."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Lopes, B., Sousa, P., Alvites, R., Branquinho, M., Sousa, A.C., Mendon\u00e7a, C., Atayde, L.M., Lu\u00eds, A.L., Varej\u00e3o, A.S.P., and Maur\u00edcio, A.C. (2022). Peripheral Nerve Injury Treatments and Advances: One Health Perspective. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23020918"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3293","DOI":"10.4081\/bam.2014.1.5","article-title":"The Biology of Long-Term Denervated Skeletal Muscle","volume":"24","author":"Carlson","year":"2014","journal-title":"Eur. J. Transl. Myol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Purslow, P.P. (2020). The Structure and Role of Intramuscular Connective Tissue in Muscle Function. Front. Physiol., 11.","DOI":"10.3389\/fphys.2020.00495"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/mus.22094","article-title":"Structure and function of the skeletal muscle extracellular matrix","volume":"44","author":"Gillies","year":"2011","journal-title":"Muscle Nerve"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.matbio.2019.01.002","article-title":"Denervation-induced skeletal muscle fibrosis is mediated by CTGF\/CCN2 independently of TGF-\u03b2","volume":"82","author":"Rebolledo","year":"2019","journal-title":"Matrix Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"251","DOI":"10.2220\/biomedres.34.251","article-title":"Regulation of connective tissue remodeling in the early phase of denervation in a rat skeletal muscle","volume":"34","author":"Ozawa","year":"2013","journal-title":"Biomed. Res."},{"key":"ref_9","first-page":"R897","article-title":"Effects of denervation and immobilization on collagen synthesis in rat skeletal muscle and tendon","volume":"254","author":"Savolainen","year":"1988","journal-title":"Am. J. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"R983","DOI":"10.1152\/ajpregu.00483.2006","article-title":"Denervation does not change the ratio of collagen I and collagen III mRNA in the extracellular matrix of muscle","volume":"292","author":"Arruda","year":"2007","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1002\/mus.880080208","article-title":"Changes in intramuscular collagen and fibronectin in denervation atrophy","volume":"8","author":"Salonen","year":"1985","journal-title":"Muscle Nerve"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.12989\/sss.2011.7.3.213","article-title":"Biomimetics of the Extracellular Matrix: An Integrated Three-Dimensional Fiber-Hydrogel Composite for Cartilage Tissue Engineering","volume":"7","author":"Coburn","year":"2011","journal-title":"Smart Struct. Syst."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Fede, C., Pirri, C., Fan, C., Petrelli, L., Guidolin, D., De Caro, R., and Stecco, C. (2021). A Closer Look at the Cellular and Molecular Components of the Deep\/Muscular Fasciae. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031411"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Singla, S.K. (1987). Nomenclature of hyaluronic acid. Biochem. J., 242.","DOI":"10.1042\/bj2420623"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1007\/s00276-011-0876-9","article-title":"Hyaluronan within fascia in the etiology of myofascial pain","volume":"33","author":"Stecco","year":"2011","journal-title":"Surg. Radiol. Anat."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1054\/jhsb.2000.0546","article-title":"The histochemical structure of the deep fascia and its structural response to surgery","volume":"26","author":"McCombe","year":"2001","journal-title":"J. Hand Surg. Br."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pratt, R.L. (2021). Hyaluronan and the Fascial Frontier. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136845"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"622","DOI":"10.12688\/f1000research.6885.1","article-title":"Viscoelastic Properties of Hyaluronan in Physiological Conditions","volume":"4","author":"Cowman","year":"2015","journal-title":"F1000Research"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1002\/pmrj.12771","article-title":"Hyaluronan homeostasis and its role in pain and muscle stiffness","volume":"14","author":"Amir","year":"2022","journal-title":"PM&R"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1023\/B:JURE.0000035851.12800.39","article-title":"Effects of reduced joint mobility on sarcomere length, collagen fibril arrangement in the endomysium, and hyaluronan in rat soleus muscle","volume":"25","author":"Okita","year":"2004","journal-title":"J. Muscle Res. Cell Motil."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Menon, R.G., Raghavan, P., and Regatte, R.R. (2019). Quantifying muscle glycosaminoglycan levels in patients with post-stroke muscle stiffness using T(1\u03c1) MRI. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-50715-x"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Fede, C., Fan, C., Pirri, C., Petrelli, L., Biz, C., Porzionato, A., Macchi, V., De Caro, R., and Stecco, C. (2022). The Effects of Aging on the Intramuscular Connective Tissue. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911061"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Fantoni, I., Biz, C., Fan, C., Pirri, C., Fede, C., Petrelli, L., Ruggieri, P., De Caro, R., and Stecco, C. (2021). Fascia Lata Alterations in Hip Osteoarthritis: An Observational Cross-Sectional Study. Life, 11.","DOI":"10.3390\/life11111136"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"26927","DOI":"10.3390\/ijms161126003","article-title":"Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats","volume":"16","author":"Wu","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2754\/avb202493020201","article-title":"The study of testosterone and tacrolimus roles on gastrocnemius muscle following experimental sciatic nerve injury in rats","volume":"93","author":"Jazinidorcheh","year":"2024","journal-title":"Acta Vet. Brno"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1002\/mus.26197","article-title":"Alterations in mitochondrial fission, fusion, and mitophagic protein expression in the gastrocnemius of mice after a sciatic nerve transection","volume":"58","author":"Graham","year":"2018","journal-title":"Muscle Nerve"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"393","DOI":"10.4040\/jkan.2009.39.3.393","article-title":"Effects of unilateral sciatic nerve injury on unaffected hindlimb muscles of rats","volume":"39","author":"Kim","year":"2009","journal-title":"J. Korean Acad. Nurs."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1177\/1099800410382291","article-title":"Hindlimb muscle atrophy occurs from peripheral nerve damage in a rat neuropathic pain model","volume":"13","author":"Choe","year":"2011","journal-title":"Biol. Res. Nurs."},{"key":"ref_29","unstructured":"Stecco, C. (2014). Functional Atlas of the Human Fascial System, Elsevier Health Sciences."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1097\/00006534-198901000-00024","article-title":"Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat","volume":"83","author":"Bain","year":"1989","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Stocco, E., Barbon, S., Faccio, D., Petrelli, L., Incendi, D., Zamuner, A., De Rose, E., Confalonieri, M., Tolomei, F., and Todros, S. (2023). Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study. Mater. Today Bio, 22.","DOI":"10.1016\/j.mtbio.2023.100761"},{"key":"ref_32","first-page":"78","article-title":"Hyaluronic Acid in Inflammation and Tissue Regeneration","volume":"28","author":"Litwiniuk","year":"2016","journal-title":"Wounds"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.cellbio.23.090506.123337","article-title":"Hyaluronan in tissue injury and repair","volume":"23","author":"Jiang","year":"2007","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.matbio.2018.08.008","article-title":"Hyaluronic acid, CD44 and RHAMM regulate myoblast behavior during embryogenesis","volume":"78\u201379","author":"Leng","year":"2019","journal-title":"Matrix Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1126\/science.abm9735","article-title":"JMJD3 activated hyaluronan synthesis drives muscle regeneration in an inflammatory environment","volume":"377","author":"Nakka","year":"2022","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ricard-Blum, S. (2011). The collagen family. Cold Spring Harb. Perspect. Biol., 3.","DOI":"10.1101\/cshperspect.a004978"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1091\/mbc.E19-12-0709","article-title":"Collagen, stiffness, and adhesion: The evolutionary basis of vertebrate mechanobiology","volume":"31","author":"Tang","year":"2020","journal-title":"Mol. Biol. Cell"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhang, W., Liu, Y., and Zhang, H. (2021). Extracellular matrix: An important regulator of cell functions and skeletal muscle development. Cell Biosci., 11.","DOI":"10.1186\/s13578-021-00579-4"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liu, J., Liao, Z., Wang, J., Xiang, H., Zhu, X., Che, X., Tang, Y., Xie, J., Mao, C., and Zhao, H. (2022). Research on skeletal muscle impact injury using a new rat model from a bioimpact machine. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.1055668"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.gaitpost.2013.05.009","article-title":"The modulation of forward propulsion, vertical support, and center of pressure by the plantarflexors during human walking","volume":"38","author":"Francis","year":"2013","journal-title":"Gait Posture"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1469-7580.2012.01511.x","article-title":"The thoracolumbar fascia: Anatomy, function and clinical considerations","volume":"221","author":"Willard","year":"2012","journal-title":"J. Anat."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/s00276-010-0757-7","article-title":"3D reconstruction of the crural and thoracolumbar fasciae","volume":"33","author":"Benetazzo","year":"2011","journal-title":"Surg. Radiol. Anat."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.neuroscience.2011.07.066","article-title":"Sensory innervation of the thoracolumbar fascia in rats and humans","volume":"194","author":"Tesarz","year":"2011","journal-title":"Neuroscience"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Chen, B., Cui, S., Xu, M., Zhang, Z., and Liu, C. (2021). Effects of Isometric Plantar-Flexion on the Lower Limb Muscle and Lumbar Tissue Stiffness. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.810250"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Langevin, H.M., Fox, J.R., Koptiuch, C., Badger, G.J., Greenan-Naumann, A.C., Bouffard, N.A., Konofagou, E.E., Lee, W.N., Triano, J.J., and Henry, S.M. (2011). Reduced thoracolumbar fascia shear strain in human chronic low back pain. BMC Musculoskelet. Disord., 12.","DOI":"10.1186\/1471-2474-12-203"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Langevin, H.M. (2021). Fascia Mobility, Proprioception, and Myofascial Pain. Life, 11.","DOI":"10.3390\/life11070668"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kodama, Y., Masuda, S., Ohmori, T., Kanamaru, A., Tanaka, M., Sakaguchi, T., and Nakagawa, M. (2023). Response to Mechanical Properties and Physiological Challenges of Fascia: Diagnosis and Rehabilitative Therapeutic Intervention for Myofascial System Disorders. Bioengineering, 10.","DOI":"10.3390\/bioengineering10040474"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"37578","DOI":"10.52965\/001c.37578","article-title":"Assessment of Motor Function in Peripheral Nerve Injury and Recovery","volume":"14","author":"John","year":"2022","journal-title":"Orthop. Rev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1089\/089771504774129955","article-title":"Methodological evaluation to analyze functional recovery after sciatic nerve injury","volume":"21","year":"2004","journal-title":"J. Neurotrauma"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.jneumeth.2008.01.022","article-title":"Is the Sciatic Functional Index always reliable and reproducible?","volume":"170","author":"Barbieri","year":"2008","journal-title":"J. Neurosci. Methods"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Stocco, E., Barbon, S., Macchi, V., Tiengo, C., Petrelli, L., Rambaldo, A., Borean, A., Capelli, S., Filippi, A., and Romanato, F. (2019). New bioresorbable wraps based on oxidized polyvinyl alcohol and leukocyte-fibrin-platelet membrane to support peripheral nerve neurorrhaphy: Preclinical comparison versus NeuraWrap. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-53812-z"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Stocco, E., Barbon, S., Lora, L., Grandi, F., Sartore, L., Tiengo, C., Petrelli, L., Dalzoppo, D., Parnigotto, P.P., and Macchi, V. (2018). Partially oxidized polyvinyl alcohol conduitfor peripheral nerve regeneration. Sci. Rep., 8.","DOI":"10.1038\/s41598-017-19058-3"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/S0142-9612(03)00504-0","article-title":"Transection of peripheral nerves, bridging strategies and effect evaluation","volume":"25","author":"Jansen","year":"2004","journal-title":"Biomaterials"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/joa.12866","article-title":"Quantification of hyaluronan in human fasciae: Variations with function and anatomical site","volume":"233","author":"Fede","year":"2018","journal-title":"J. Anat."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1002\/ca.23072","article-title":"The fasciacytes: A new cell devoted to fascial gliding regulation","volume":"31","author":"Stecco","year":"2018","journal-title":"Clin. Anat."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Fede, C., Coldebella, L., Petrelli, L., Bassetto, F., Tiengo, C., and Stecco, C. (2024). Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren\u2019s Palmar Aponeurosis and Innovative Clinical Implications. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25136865"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/BF00306130","article-title":"Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions","volume":"5","author":"Scott","year":"1965","journal-title":"Histochemie"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. 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