{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:57:43Z","timestamp":1779908263656,"version":"3.53.1"},"reference-count":61,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T00:00:00Z","timestamp":1724198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Technology Development Program","award":["S3383209"],"award-info":[{"award-number":["S3383209"]}]},{"name":"National University Development Project at Jeonbuk National University","award":["S3383209"],"award-info":[{"award-number":["S3383209"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the current study, two Salmonella Typhimurium strains, JOL 912 and JOL 1800, were engineered from the wild-type JOL 401 strain through in-frame deletions of the lon and cpxR genes, with JOL 1800 also lacking rfaL. These deletions significantly attenuated the strains, impairing their intracellular survival and creating unique immunological profiles. This study investigates the response of these strains to various abiotic stress conditions commonly experienced in vivo, including temperature, acidity, osmotic, and oxidative stress. Notably, cold stress induced a non-significant trend towards increased invasion by Salmonella compared to other stressors. Despite the observed attenuation, no significant alterations in entry mechanisms (trigger vs. zipper) were noted between these strains, although variations were evident depending on the host cell type. Both strains effectively localized within the cytoplasm, demonstrating their ability to invade and interact with the intracellular environment. Immunologically, JOL 912 elicited a robust response, marked by substantial activation of nuclear factor kappa B (NF-kB), and chemokines, interleukin 8 (CXCL 8) and interleukin 10 (CXCL 10), comparable to the wild-type JOL 401 (over a fourfold increase compared to JOL 1800). In contrast, JOL 1800 exhibited a minimal immune response. Additionally, these attenuations influenced the expression of cyclins D1 and B1 and caspases 3 and 7, indicating cell cycle arrest at the G2\/M phase and promotion of the G0\/G1 to S phase transition, alongside apoptosis in infected cells. These findings provide valuable insights into the mechanisms governing the association, internalization, and survival of Salmonella mutants, enhancing our understanding of their regulatory effects on host cell physiology.<\/jats:p>","DOI":"10.3390\/ijms25169056","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T05:16:24Z","timestamp":1724649384000},"page":"9056","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Intracellular Survival and Pathogenicity Modulation of Salmonella Lon, CpxR, and RfaL Mutants Used as Live Bacterial Vectors under Abiotic Stress, Unveiling the Link between Stress Response and Virulence in Epithelial Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9638-1615","authenticated-orcid":false,"given":"Perumalraja","family":"Kirthika","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Senevirathne","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungwoo","family":"Park","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram Prasad","family":"Aganja","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In-Shik","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Institute of Animal Transplantation, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5193-508X","authenticated-orcid":false,"given":"Hyun-Jin","family":"Tae","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Institute of Animal Transplantation, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-2207","authenticated-orcid":false,"given":"John Hwa","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1590\/S1517-83822014000400001","article-title":"Live bacterial vaccine vectors: An overview","volume":"45","author":"Zangirolami","year":"2014","journal-title":"Braz. J. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1038\/s41467-024-46775-x","article-title":"A live attenuated vaccine to prevent severe neonatal Escherichia coli K1 infections","volume":"15","author":"Sereme","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ji, H.J., Jang, A.Y., Song, J.Y., Ahn, K.B., Han, S.H., Bang, S.J., Jung, H.K., Hur, J., and Seo, H.S. (2022). Development of Live Attenuated Salmonella Typhimurium Vaccine Strain Using Radiation Mutation Enhancement Technology (R-MET). Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.931052"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Flickinger, J.C., Rodeck, U., and Snook, A.E. (2018). Listeria monocytogenes as a Vector for Cancer Immunotherapy: Current Understanding and Progress. Vaccines, 6.","DOI":"10.3390\/vaccines6030048"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1038\/s41388-022-02222-z","article-title":"Safety and efficacy of prophylactic and therapeutic vaccine based on live-attenuated Listeria monocytogenes in hepatobiliary cancers","volume":"41","author":"Hochnadel","year":"2022","journal-title":"Oncogene"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"MacLennan, C.A., Grow, S., Ma, L.F., and Steele, A.D. (2022). The Shigella Vaccines Pipeline. Vaccines, 10.","DOI":"10.3390\/vaccines10091376"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21315\/mjms2022.29.2.1","article-title":"Live, Genetically Attenuated, Cold-Chain-Free Cholera Vaccine-A Research and Development Journey: Light at the End of a Long Tunnel","volume":"29","author":"Ravichandran","year":"2022","journal-title":"Malays. J. Med. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mohamad, A., Mursidi, F.A., Zamri-Saad, M., Amal, M.N.A., Annas, S., Monir, M.S., Loqman, M., Hairudin, F., Al-Saari, N., and Ina-Salwany, M.Y. (2022). Laboratory and Field Assessments of Oral Vibrio Vaccine Indicate the Potential for Protection against Vibriosis in Cultured Marine Fishes. Animals, 12.","DOI":"10.3390\/ani12020133"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Uriza, P.J., Trautman, C., Palomino, M.M., Fina Martin, J., Ruzal, S.M., Roset, M.S., and Briones, G. (2020). Development of an Antigen Delivery Platform Using Lactobacillus acidophilus Decorated With Heterologous Proteins: A Sheep in Wolf\u2019s Clothing Story. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.509380"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zhou, M., Tang, Y., Xu, W., Hao, X., Li, Y., Huang, S., Xiang, D., and Wu, J. (2023). Bacteria-based immunotherapy for cancer: A systematic review of preclinical studies. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1140463"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/978-1-0716-0795-4_17","article-title":"Attenuation Methods for Live Vaccines","volume":"2183","author":"Hajra","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1128\/CVI.05265-11","article-title":"Long-term antibody and immune memory response induced by pulmonary delivery of the influenza Iscomatrix vaccine","volume":"19","author":"Vujanic","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1586\/14760584.2013.825454","article-title":"Salmonella as a vaccine delivery vehicle","volume":"12","author":"Roland","year":"2013","journal-title":"Expert Rev. Vaccines"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Senevirathne, A., Aganja, R.P., Hewawaduge, C., and Lee, J.H. (2023). Inflammation-Related Immune-Modulatory SLURP1 Prevents the Proliferation of Human Colon Cancer Cells, and Its Delivery by Salmonella Demonstrates Cross-Species Efficacy against Murine Colon Cancer. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15102462"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0378-1135(96)01279-5","article-title":"Evaluation of an aroA mutant Salmonella typhimurium vaccine in chickens using modified semisolid Rappaport Vassiliadis medium to monitor faecal shedding","volume":"54","author":"Tan","year":"1997","journal-title":"Vet. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.vaccine.2010.11.039","article-title":"Evaluation of safety and protection efficacy on cpxR and lon deleted mutant of Salmonella Gallinarum as a live vaccine candidate for fowl typhoid","volume":"29","author":"Matsuda","year":"2011","journal-title":"Vaccine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1200\/JCO.2002.20.1.142","article-title":"Phase I study of the intravenous administration of attenuated Salmonella typhimurium to patients with metastatic melanoma","volume":"20","author":"Toso","year":"2002","journal-title":"J. Clin. Oncol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1080\/21645515.2018.1491499","article-title":"Deletions in guaBA and htrA but not clpX or rfaL constitute a live-attenuated vaccine strain of Salmonella Newport to protect against serogroup C(2-)C(3)Salmonella in mice","volume":"15","author":"Fuche","year":"2019","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1093\/infdis\/156.1.167","article-title":"Construction of defined galE mutants of Salmonella for use as vaccines","volume":"156","author":"Hone","year":"1987","journal-title":"J. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hussain, A., Ong, E.B.B., Balaram, P., Ismail, A., and Kien, P.K. (2023). Deletion of Salmonella enterica serovar Typhi tolC reduces bacterial adhesion and invasion toward host cells. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1301478"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Belousov, M.V., Kosolapova, A.O., Fayoud, H., Sulatsky, M.I., Sulatskaya, A.I., Romanenko, M.N., Bobylev, A.G., Antonets, K.S., and Nizhnikov, A.A. (2023). OmpC and OmpF Outer Membrane Proteins of Escherichia coli and Salmonella enterica Form Bona Fide Amyloids. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242115522"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"38780","DOI":"10.18632\/oncotarget.16353","article-title":"A consensus-hemagglutinin-based vaccine delivered by an attenuated Salmonella mutant protects chickens against heterologous H7N1 influenza virus","volume":"8","author":"Hyoung","year":"2017","journal-title":"Oncotarget"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"25441","DOI":"10.18632\/oncotarget.25192","article-title":"Engineering of a rough auxotrophic mutant Salmonella Typhimurium for effective delivery","volume":"9","author":"Lalsiamthara","year":"2018","journal-title":"Oncotarget"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1080\/19490976.2020.1777923","article-title":"Deletion of the lon gene augments expression of Salmonella Pathogenicity Island (SPI)-1 and metal ion uptake genes leading to the accumulation of bactericidal hydroxyl radicals and host pro-inflammatory cytokine-mediated rapid intracellular clearance","volume":"11","author":"Kirthika","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Shetty, D., Abrahante, J.E., Chekabab, S.M., Wu, X., Korber, D.R., and Vidovic, S. (2019). Role of CpxR in Biofilm Development: Expression of Key Fimbrial, O-Antigen and Virulence Operons of Salmonella Enteritidis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20205146"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.chom.2014.12.003","article-title":"Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton","volume":"17","author":"Lhocine","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.mib.2021.08.007","article-title":"Salmonella effector driven invasion of the gut epithelium: Breaking in and setting the house on fire","volume":"64","author":"Fattinger","year":"2021","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.cell.2007.06.049","article-title":"A common mechanism of cellular death induced by bactericidal antibiotics","volume":"130","author":"Kohanski","year":"2007","journal-title":"Cell"},{"key":"ref_30","first-page":"2698","article-title":"Metal peroxides for cancer treatment","volume":"6","author":"He","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.clim.2004.07.014","article-title":"Targeting of the actin cytoskeleton during infection by Salmonella strains","volume":"114","author":"Guiney","year":"2005","journal-title":"Clin. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2158663","DOI":"10.1080\/21505594.2022.2158663","article-title":"Epithelial cell invasion by salmonella typhimurium induces modulation of genes controlled by aryl hydrocarbon receptor signaling and involved in extracellular matrix biogenesis","volume":"14","author":"Chausse","year":"2023","journal-title":"Virulence"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/cr.2010.45","article-title":"Rck of Salmonella enterica, subspecies enterica serovar enteritidis, mediates zipper-like internalization","volume":"20","author":"Rosselin","year":"2010","journal-title":"Cell Res."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Barilleau, E., Vedrine, M., Koczerka, M., Burlaud-Gaillard, J., Kempf, F., Grepinet, O., Virlogeux-Payant, I., Velge, P., and Wiedemann, A. (2021). Investigation of the invasion mechanism mediated by the outer membrane protein PagN of Salmonella Typhimurium. BMC Microbiol., 21.","DOI":"10.1186\/s12866-021-02187-1"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"17023","DOI":"10.1038\/sigtrans.2017.23","article-title":"NF-kappaB signaling in inflammation","volume":"2","author":"Liu","year":"2017","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Sokol, C.L., and Luster, A.D. (2015). The chemokine system in innate immunity. Cold Spring Harb. Perspect. Biol., 7.","DOI":"10.1101\/cshperspect.a016303"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3901","DOI":"10.1128\/IAI.71.7.3901-3908.2003","article-title":"Activation of toll-like receptor 2 (TLR2) and TLR4\/MD2 by Neisseria is independent of capsule and lipooligosaccharide (LOS) sialylation but varies widely among LOS from different strains","volume":"71","author":"Pridmore","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e51280","DOI":"10.15252\/embr.202051280","article-title":"Toll-like receptor 4 is activated by platinum and contributes to cisplatin-induced ototoxicity","volume":"22","author":"Babolmorad","year":"2021","journal-title":"EMBO Rep."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Yan, J., Racaud-Sultan, C., Pezier, T., Edir, A., Rolland, C., Claverie, C., Burlaud-Gaillard, J., Olivier, M., Velge, P., and Lacroix-Lamande, S. (2024). Intestinal organoids to model Salmonella infection and its impact on progenitors. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-65485-4"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Mambu, J., Barilleau, E., Fragnet-Trapp, L., Le Vern, Y., Olivier, M., Sadrin, G., Grepinet, O., Taieb, F., Velge, P., and Wiedemann, A. (2020). Rck of Salmonella Typhimurium Delays the Host Cell Cycle to Facilitate Bacterial Invasion. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.586934"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1080\/15548627.2021.1999561","article-title":"Dysregulated endolysosomal trafficking in cells arrested in the G(1) phase of the host cell cycle impairs Salmonella vacuolar replication","volume":"18","author":"Lisowski","year":"2022","journal-title":"Autophagy"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Hajam, I.A., and Lee, J.H. (2017). An Influenza HA and M2e Based Vaccine Delivered by a Novel Attenuated Salmonella Mutant Protects Mice against Homologous H1N1 Infection. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00872"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1128\/CVI.00407-10","article-title":"Enhancement of immune responses by an attenuated Salmonella enterica serovar Typhimurium strain secreting an Escherichia coli heat-labile enterotoxin B subunit protein as an adjuvant for a live Salmonella vaccine candidate","volume":"18","author":"Hur","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/mbo3.28","article-title":"Multiplicity of Salmonella entry mechanisms, a new paradigm for Salmonella pathogenesis","volume":"1","author":"Velge","year":"2012","journal-title":"Microbiologyopen"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1128\/IAI.72.4.2002-2013.2004","article-title":"Lon protease activity causes down-regulation of Salmonella pathogenicity island 1 invasion gene expression after infection of epithelial cells","volume":"72","author":"Boddicker","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6943","DOI":"10.1128\/AEM.01994-14","article-title":"Genetic mechanisms underlying the pathogenicity of cold-stressed Salmonella enterica serovar typhimurium in cultured intestinal epithelial cells","volume":"80","author":"Shah","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"711","DOI":"10.4161\/19490976.2014.983774","article-title":"Cronobacter sakazakii: Stress survival and virulence potential in an opportunistic foodborne pathogen","volume":"5","author":"Feeney","year":"2014","journal-title":"Gut Microbes"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Roche, S.M., Holbert, S., Trotereau, J., Schaeffer, S., Georgeault, S., Virlogeux-Payant, I., and Velge, P. (2018). Salmonella Typhimurium Invalidated for the Three Currently Known Invasion Factors Keeps Its Ability to Invade Several Cell Models. Front. Cell. Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00273"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0962-8924(00)88956-4","article-title":"Phagocytosis by zippers and triggers","volume":"5","author":"Swanson","year":"1995","journal-title":"Trends Cell Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1111\/j.1462-5822.2007.00992.x","article-title":"Mechanisms of Salmonella entry into host cells","volume":"9","author":"Ly","year":"2007","journal-title":"Cell. Microbiol"},{"key":"ref_51","first-page":"967","article-title":"Epidermal Growth Factor Receptor: Key to Selective Intracellular Delivery","volume":"85","author":"Rosenkranz","year":"2020","journal-title":"Biochemistry"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Castanheira, S., and Garcia-Del Portillo, F. (2017). Salmonella Populations inside Host Cells. Front. Cell. Infect. Microbiol., 7.","DOI":"10.3389\/fcimb.2017.00432"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/CMR.00046-08","article-title":"Pathogen recognition and inflammatory signaling in innate immune defenses","volume":"22","author":"Mogensen","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5691","DOI":"10.1007\/s00253-016-7557-x","article-title":"Lactic acid bacteria as mucosal delivery vehicles: A realistic therapeutic option","volume":"100","author":"Wang","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1023\/A:1026422111457","article-title":"Cell cycle G2\/M arrest and activation of cyclin-dependent kinases associated with low-dose paclitaxel-induced sub-G1 apoptosis","volume":"2","author":"Shu","year":"1997","journal-title":"Apoptosis"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4718","DOI":"10.1038\/ncomms5718","article-title":"Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection","volume":"5","author":"Maudet","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.mimet.2009.09.025","article-title":"Changes of physiological and biochemical properties of Salmonella enterica serovar Typhimurium by deletion of cpxR and lon genes using allelic exchange method","volume":"79","author":"Kim","year":"2009","journal-title":"J. Microbiol. Methods"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7281","DOI":"10.1128\/AEM.02621-13","article-title":"Preadaptation to cold stress in Salmonella enterica serovar Typhimurium increases survival during subsequent acid stress exposure","volume":"79","author":"Shah","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_59","first-page":"e51759","article-title":"High-throughput assay to phenotype Salmonella enterica Typhimurium association, invasion, and replication in macrophages","volume":"90","author":"Wu","year":"2014","journal-title":"J. Vis. Exp."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/16\/9056\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:40:13Z","timestamp":1760110813000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/16\/9056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,21]]},"references-count":61,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["ijms25169056"],"URL":"https:\/\/doi.org\/10.3390\/ijms25169056","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,21]]}}}