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Neurochemistry [CAEN]","award":["APQ-04985-23"],"award-info":[{"award-number":["APQ-04985-23"]}]},{"name":"Society for Neurochemistry [CAEN]","award":["425669\/2018-1"],"award-info":[{"award-number":["425669\/2018-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Neurodegenerative disorders, including traumatic injuries to the central nervous system (CNS) and neurodegenerative diseases, are characterized by early axonal damage, which does not regenerate in the adult mammalian CNS, leading to permanent neurological deficits. One of the primary causes of the loss of regenerative ability is thought to be a developmental decline in neurons\u2019 intrinsic capability for axon growth. Different molecules are involved in the developmental loss of the ability for axon regeneration, including many transcription factors. However, the function of microRNAs (miRNAs), which are also modulators of gene expression, in axon re-growth is still unclear. Among the various miRNAs recently identified with roles in the CNS, miR-17, which is highly expressed during early development, emerges as a promising target to promote axon regeneration. Here, we used adeno-associated viral (AAV) vectors to overexpress miR-17 (AAV.miR-17) in primary cortical neurons and evaluate its effects on neurite and axon regeneration in vitro. Although AAV.miR-17 had no significant effect on neurite outgrowth and arborization, it significantly enhances neurite regeneration after scratch lesion and axon regeneration after axotomy of neurons cultured in microfluidic chambers. Target prediction and functional annotation analyses suggest that miR-17 regulates gene expression associated with autophagy and cell metabolism. Our findings suggest that miR-17 promotes regenerative response and thus could mitigate neurodegenerative effects.<\/jats:p>","DOI":"10.3390\/ijms25169057","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T05:16:24Z","timestamp":1724649384000},"page":"9057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["AAV-Mediated Expression of miR-17 Enhances Neurite and Axon Regeneration In Vitro"],"prefix":"10.3390","volume":"25","author":[{"given":"Raquel Alves","family":"Almeida","sequence":"first","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Pres. Ant\u00f4nio Carlos, 6627, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Gomes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Pres. Ant\u00f4nio Carlos, 6627, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Ulysses Souza","family":"Matos","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Pres. Ant\u00f4nio Carlos, 6627, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Meireles","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Pres. Ant\u00f4nio Carlos, 6627, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Pimenta","family":"Braga","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4959-2169","authenticated-orcid":false,"given":"Lucas","family":"Caldi Gomes","sequence":"additional","affiliation":[{"name":"Clinical Department of Neurology, TUM School of Medicine, Rechts der Isar Hospital, Technical University of Munich, 81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5390-5266","authenticated-orcid":false,"given":"Erika Cristina","family":"Jorge","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Pres. Ant\u00f4nio Carlos, 6627, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico Marianetti","family":"Soriani","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Michel","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medicine G\u00f6ttingen, 37075 G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8161-786X","authenticated-orcid":false,"given":"Vinicius Toledo","family":"Ribas","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Pres. Ant\u00f4nio Carlos, 6627, Belo Horizonte 31279-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,21]]},"reference":[{"key":"ref_1","first-page":"515","article-title":"Diverse cellular and molecular modes of axon degeneration","volume":"24","author":"Neukomm","year":"2014","journal-title":"Cell Press"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1126\/science.1068428","article-title":"Amacrine-Signaled Loss of Intrinsic Axon Growth Ability by Retinal Ganglion Cells","volume":"296","author":"Goldberg","year":"2002","journal-title":"Science"},{"key":"ref_3","first-page":"617","article-title":"Glial inhibition of CNS axon regeneration","volume":"7","author":"Yiu","year":"2006","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8318","DOI":"10.1523\/JNEUROSCI.23-23-08318.2003","article-title":"The Developmental Loss of the Ability of Purkinje Cells to Regenerate Their Axons Occurs in the Absence of Myelin: Na In Vitro Model to Prevent Myelination","volume":"23","author":"Wehrle","year":"2003","journal-title":"J. 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