{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:42:42Z","timestamp":1790876562314,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Grant Agency of Slovak Republic VEGA","award":["1\/0661\/24"],"award-info":[{"award-number":["1\/0661\/24"]}]},{"name":"Scientific Grant Agency of Slovak Republic VEGA","award":["1\/0429\/21"],"award-info":[{"award-number":["1\/0429\/21"]}]},{"name":"Scientific Grant Agency of Slovak Republic VEGA","award":["APVV-23-0349"],"award-info":[{"award-number":["APVV-23-0349"]}]},{"DOI":"10.13039\/501100005357","name":"Research and Development Agency","doi-asserted-by":"publisher","award":["1\/0661\/24"],"award-info":[{"award-number":["1\/0661\/24"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Research and Development Agency","doi-asserted-by":"publisher","award":["1\/0429\/21"],"award-info":[{"award-number":["1\/0429\/21"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Research and Development Agency","doi-asserted-by":"publisher","award":["APVV-23-0349"],"award-info":[{"award-number":["APVV-23-0349"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Several novel copper (II) complexes of reduced Schiff bases containing fluoride substituents were prepared and structurally characterized by single-crystal X-ray diffraction. The complexes exhibited diverse structures, with the central atom in distorted tetrahedral geometry. The biological effects of the products were evaluated, specifically their cytotoxicity, antimicrobial, and antiurease activities, as well as affinity for albumin (BSA) and DNA (ct-DNA). The complexes showed marked cytotoxic activities in the HepG2 hepatocellular carcinoma cell line, considerably higher than the standard cisplatin. The cytotoxicity depended significantly on the substitution pattern. The best activity was observed in the complex with a trifluoromethyl group in position 4 of the benzene ring\u2014the dichloro[(\u00b1)-trans-N,N\u2032-bis-(4-trifluoromethylbenzyl)-cyclohexane-1,2-diamine]copper (II) complex, whose activity (IC50 28.7 \u03bcM) was higher than that of the free ligand and markedly better than the activity of the standard cisplatin (IC50 336.8 \u03bcM). The same complex also showed the highest antimicrobial effect in vitro. The affinity of the complexes towards bovine serum albumin (BSA) and calf thymus DNA (ct-DNA) was established as well, indicating only marginal differences between the complexes. In addition, all complexes were shown to be excellent inhibitors of the enzyme urease, with the IC50 values in the lower micromolar region.<\/jats:p>","DOI":"10.3390\/ijms25179166","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T05:16:24Z","timestamp":1724649384000},"page":"9166","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Novel Copper (II) Complexes with Fluorine-Containing Reduced Schiff Base Ligands Showing Marked Cytotoxicity in the HepG2 Cancer Cell Line"],"prefix":"10.3390","volume":"25","author":[{"given":"Bianka","family":"Obo\u0148ov\u00e1","sequence":"first","affiliation":[{"name":"Department Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jindra","family":"Valentov\u00e1","sequence":"additional","affiliation":[{"name":"Department Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5336-9819","authenticated-orcid":false,"given":"Miroslava","family":"Liteck\u00e1","sequence":"additional","affiliation":[{"name":"Department of Materials Chemistry, Institute of Inorganic Chemistry of the CAS, Husinec-\u0158e\u017e \u010d.p. 1001, 250 68 \u0158e\u017e, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8732-7054","authenticated-orcid":false,"given":"\u013dudmila","family":"Pa\u0161kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5098-4122","authenticated-orcid":false,"given":"Jana","family":"Hricov\u00edniov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7956-5845","authenticated-orcid":false,"given":"Andrea","family":"Bilkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franti\u0161ek","family":"Bilka","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branislav","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"NMR Laboratory, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladislav","family":"Habala","sequence":"additional","affiliation":[{"name":"Department Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odboj\u00e1rov 10, 832 32 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12264","DOI":"10.3390\/molecules181012264","article-title":"Schiff bases: A short survey on an evergreen chemistry tool","volume":"18","author":"Qin","year":"2013","journal-title":"Molecules"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101179","DOI":"10.1016\/j.rechem.2023.101179","article-title":"Schiff bases-based metallo complexes and their crucial role in the realm of pharmacology. 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