{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:34:23Z","timestamp":1782282863001,"version":"3.54.5"},"reference-count":39,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T00:00:00Z","timestamp":1724544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Uniformed Services the Armed Forces Radiobiology Research Institute\/University of the Health Sciences","award":["AFR-12080"],"award-info":[{"award-number":["AFR-12080"]}]},{"name":"Uniformed Services the Armed Forces Radiobiology Research Institute\/University of the Health Sciences","award":["DZRO-FVZ22-ZHN II"],"award-info":[{"award-number":["DZRO-FVZ22-ZHN II"]}]},{"name":"Ministry of Defence of Czech Republic","award":["AFR-12080"],"award-info":[{"award-number":["AFR-12080"]}]},{"name":"Ministry of Defence of Czech Republic","award":["DZRO-FVZ22-ZHN II"],"award-info":[{"award-number":["DZRO-FVZ22-ZHN II"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the last decade, geopolitical instability across the globe has increased the risk of a large-scale radiological event, when radiation biomarkers would be needed for an effective triage of an irradiated population. Ionizing radiation elicits a complex response in the proteome, genome, and metabolome and hence can be leveraged as rapid and sensitive indicators of irradiation-induced damage. We analyzed the plasma of total-body irradiated (TBI) leukemia patients (n = 24) and nonhuman primates (NHPs; n = 10) before and 24 h after irradiation, and we performed a global metabolomic study aiming to provide plasma metabolites as candidate radiation biomarkers for biological dosimetry. Peripheral blood samples were collected according to the appropriate ethical approvals, and metabolites were extracted and analyzed by liquid chromatography mass spectrometry. We identified an array of metabolites significantly altered by irradiation, including bilirubin, cholesterol, and 18-hydroxycorticosterone, which were detected in leukemia patients and NHPs. Pathway analysis showed overlapping perturbations in steroidogenesis, porphyrin metabolism, and steroid hormone biosynthesis and metabolism. Additionally, we observed dysregulation in bile acid biosynthesis and tyrosine metabolism in the TBI patient cohort. This investigation is, to our best knowledge, among the first to provide valuable insights into a comparison between human and NHP irradiation models. The findings from this study could be leveraged for translational biological dosimetry.<\/jats:p>","DOI":"10.3390\/ijms25179208","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T07:53:37Z","timestamp":1724658817000},"page":"9208","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Radiation Signature in Plasma Metabolome of Total-Body Irradiated Nonhuman Primates and Clinical Patients"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8262-5703","authenticated-orcid":false,"given":"Ales","family":"Tichy","sequence":"first","affiliation":[{"name":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, 662 10 Brno, Czech Republic"},{"name":"Biomedical Research Centre, University Hospital Hradec Kr\u00e1lov\u00e9, 500 05 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2108-2274","authenticated-orcid":false,"given":"Alana D.","family":"Carpenter","sequence":"additional","affiliation":[{"name":"Division of Radioprotectants, Department of Pharmacology and Molecular Therapeutics, F. Edward H\u00e9bert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9200-1016","authenticated-orcid":false,"given":"Yaoxiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Rydlova","sequence":"additional","affiliation":[{"name":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, 662 10 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3263-4107","authenticated-orcid":false,"given":"Pavel","family":"Rehulka","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Pathology, Faculty of Military Health Sciences, University of Defence, 500 01 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marketa","family":"Markova","sequence":"additional","affiliation":[{"name":"Department of Haematology and Blood Transfusion, University Hospital Na Bulovce, 128 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2339-6197","authenticated-orcid":false,"given":"Marcela","family":"Milanova","sequence":"additional","affiliation":[{"name":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, 662 10 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3542-9080","authenticated-orcid":false,"given":"Vojtech","family":"Chmil","sequence":"additional","affiliation":[{"name":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, 662 10 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4877-7583","authenticated-orcid":false,"given":"Amrita K.","family":"Cheema","sequence":"additional","affiliation":[{"name":"Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA"},{"name":"Department of Biochemistry, Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 2057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6631-3849","authenticated-orcid":false,"given":"Vijay K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Radioprotectants, Department of Pharmacology and Molecular Therapeutics, F. Edward H\u00e9bert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.mrrev.2017.01.001","article-title":"Ionizing radiation biomarkers in epidemiological studies\u2014An update","volume":"771","author":"Hall","year":"2017","journal-title":"Mutat. Res. Rev. Mutat. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1080\/09553002.2016.1269218","article-title":"Metabolomic applications in radiation biodosimetry: Exploring radiation effects through small molecules","volume":"93","author":"Pannkuk","year":"2017","journal-title":"Int. J. Radiat. 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