{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:19:05Z","timestamp":1790695145336,"version":"4.1.0"},"reference-count":81,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-66\/2024-03\/200007"],"award-info":[{"award-number":["451-03-66\/2024-03\/200007"]}]},{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-66\/2024-03\/200017"],"award-info":[{"award-number":["451-03-66\/2024-03\/200017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In patients with Alzheimer\u2019s disease (AD) and in animal models, the increased accumulation of amyloid \u03b2 (A\u03b2) in retinal blood vessels strongly correlates with brain amyloid deposits and cognitive decline. The accumulation of A\u03b2 in blood vessels may result from impaired transcytosis and a dysfunctional ocular glymphatic system in AD. High-dose fish oil (FO) supplementation has been shown to significantly change the expression of major facilitator superfamily domain-containing protein 2a (Mfsd2a), a key regulator of transcytosis, and Aquaporin 4 (Aqp4), an essential component of the glymphatic system in the retinas of WT mice. We examined the expression of Mfsd2a and Aqp4 in the retinas of 4-month-old 5xFAD female mice supplemented with high-dose FO for three weeks. There was a significant increase in Mfsd2a expression in 5xFAD retinas supplemented with FO compared to control 5xFAD mice. Additionally, the increase in Aqp4 expression observed in 4-month-old 5xFAD retinas, indicative of an impaired glymphatic system, was significantly decreased. Simultaneously, A\u03b2 accumulation in 5xFAD retinal blood vessels was reduced following FO supplementation. These findings suggest that high-dose FO supplementation could serve as an adjunct in developing new treatments aimed at improving the regulation of transcytosis or the function of the glymphatic system in the AD retina.<\/jats:p>","DOI":"10.3390\/ijms25179400","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T11:33:37Z","timestamp":1724931217000},"page":"9400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Impact of High-Dose Fish Oil Supplementation on Mfsd2a, Aqp4, and Amyloid-\u03b2 Expression in Retinal Blood Vessels of 5xFAD Alzheimer\u2019s Mouse Model"],"prefix":"10.3390","volume":"25","author":[{"given":"Irena","family":"Jovanovic Macura","sequence":"first","affiliation":[{"name":"Institute for Biological Research \u201cSinisa Stankovic\u201d, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9799-0335","authenticated-orcid":false,"given":"Desanka","family":"Milanovic","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSinisa Stankovic\u201d, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7247-1034","authenticated-orcid":false,"given":"Vesna","family":"Tesic","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSinisa Stankovic\u201d, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Major","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0941-6988","authenticated-orcid":false,"given":"Milka","family":"Perovic","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSinisa Stankovic\u201d, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4234-7275","authenticated-orcid":false,"given":"Miroslav","family":"Adzic","sequence":"additional","affiliation":[{"name":"Vinca\u2013Institute for Nuclear Sciences, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7977-3166","authenticated-orcid":false,"given":"Sanja","family":"Ivkovic","sequence":"additional","affiliation":[{"name":"Vinca\u2013Institute for Nuclear Sciences, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0197-4580(99)00110-4","article-title":"Vascular risk factors for Alzheimer\u2019s disease: An epidemiologic perspective","volume":"21","author":"Breteler","year":"2000","journal-title":"Neurobiol. 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