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It primarily causes fetal microcephaly and neuronal defects through maternal transmission and induces neurological complications in adults. Recent studies report elevated proinflammatory cytokines and persistent neurological alterations post recovery, but the in vivo mechanisms remain unclear. In our study, viral RNA loads in the brains of mice infected with ZIKV peaked at 7 days post infection and returned to baseline by day 21, indicating recovery. RNA sequencing of the cerebral cortex at 7 and 21 days revealed upregulated genes related to neuroinflammation and microglial activation. Histological analyses indicated neuronal cell death and altered neurite morphology owing to severe neuroinflammation. Additionally, sustained microglial activation was associated with increased phospho-Tau levels, constituting a marker of neurodegeneration. These findings highlight how persistent microglial activation leads to neuronal dysfunction post ZIKV recovery, providing insights into the molecular pathogenesis of ZIKV-induced brain abnormalities.<\/jats:p>","DOI":"10.3390\/ijms25179451","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T09:39:32Z","timestamp":1725010772000},"page":"9451","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Sustained Microglial Activation Promotes Synaptic Loss and Neuronal Dysfunction after Recovery from ZIKV Infection"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8109-7276","authenticated-orcid":false,"given":"Nahyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanul","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Bio-Industrial Technologies, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9894-4362","authenticated-orcid":false,"given":"Uijin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1681-5557","authenticated-orcid":false,"given":"Young Bong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Republic of Korea"},{"name":"Department of Bio-Industrial Technologies, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8553-2107","authenticated-orcid":false,"given":"Ha Youn","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika virus. I. Isolations and serological specificity","volume":"46","author":"Dick","year":"1952","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1016\/S1473-3099(16)30318-8","article-title":"Association between Zika virus infection and microcephaly in Brazil, January to May, 2016: Preliminary report of a case-control study","volume":"16","author":"Rodrigues","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Sher, A.A., Glover, K.K.M., and Coombs, K.M. (2019). Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00596"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1001\/jamaneurol.2017.1703","article-title":"Neurologic Complications Associated With the Zika Virus in Brazilian Adults","volume":"74","author":"Frontera","year":"2017","journal-title":"JAMA Neurol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1177\/1352458518781992","article-title":"Zika virus found in brain tissue of a multiple sclerosis patient undergoing an acute disseminated encephalomyelitis-like episode","volume":"25","author":"Lima","year":"2019","journal-title":"Mult. Scler. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s00430-015-0445-7","article-title":"Cytokine kinetics of Zika virus-infected patients from acute to reconvalescent phase","volume":"205","author":"Tappe","year":"2016","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.autrev.2017.02.002","article-title":"Autoimmunity in Guillain-Barr\u00e9 syndrome associated with Zika virus infection and beyond","volume":"16","author":"Monsalve","year":"2017","journal-title":"Autoimmun. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3890","DOI":"10.1038\/s41467-019-11866-7","article-title":"Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice","volume":"10","author":"Figueiredo","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1002\/acn3.575","article-title":"Impact of Zika Virus on adult human brain structure and functional organization","volume":"5","author":"Wirsich","year":"2018","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1016\/S0140-6736(16)00644-9","article-title":"Acute myelitis due to Zika virus infection","volume":"387","author":"Mecharles","year":"2016","journal-title":"Lancet"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/s41426-018-0151-9","article-title":"Long-term clinical outcomes of Zika-associated Guillain-Barre syndrome","volume":"7","author":"Chang","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.stem.2016.04.009","article-title":"Understanding How Zika Virus Enters and Infects Neural Target Cells","volume":"18","author":"Miner","year":"2016","journal-title":"Cell Stem Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1523\/JNEUROSCI.3124-16.2017","article-title":"Zika Virus Targeting in the Developing Brain","volume":"37","author":"Mao","year":"2017","journal-title":"J. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7117","DOI":"10.1073\/pnas.1719266115","article-title":"Human induced pluripotent stem cell-derived glial cells and neural progenitors display divergent responses to Zika and dengue infections","volume":"115","author":"Muffat","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/nn1472","article-title":"ATP mediates rapid microglial response to local brain injury in vivo","volume":"8","author":"Davalos","year":"2005","journal-title":"Nat. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1124\/jpet.102.035048","article-title":"Role of microglia in inflammation-mediated neurodegenerative diseases: Mechanisms and strategies for therapeutic intervention","volume":"304","author":"Liu","year":"2003","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s12974-015-0458-6","article-title":"Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes","volume":"12","author":"Marinelli","year":"2015","journal-title":"J. Neuroinflamm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1523\/JNEUROSCI.1136-17.2017","article-title":"Microglia-Mediated Synapse Loss in Alzheimer\u2019s Disease","volume":"38","author":"Rajendran","year":"2018","journal-title":"J. Neurosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1742-2094-7-56","article-title":"LPS-induced inflammation exacerbates phospho-tau pathology in rTg4510 mice","volume":"7","author":"Lee","year":"2010","journal-title":"J. Neuroinflamm."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1242\/jcs.01558","article-title":"Tau phosphorylation in neuronal cell function and dysfunction","volume":"117","author":"Johnson","year":"2004","journal-title":"J. Cell Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bachiller, S., Jimenez-Ferrer, I., Paulus, A., Yang, Y., Swanberg, M., Deierborg, T., and Boza-Serrano, A. (2018). Microglia in Neurological Diseases: A Road Map to Brain-Disease Dependent-Inflammatory Response. Front. Cell Neurosci., 12.","DOI":"10.3389\/fncel.2018.00488"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1038\/s41593-018-0242-x","article-title":"Microglia in neurodegeneration","volume":"21","author":"Hickman","year":"2018","journal-title":"Nat. Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1093\/infdis\/jiy129","article-title":"NLRP3 inflammasome activation mediates Zika virus\u2013associated inflammation","volume":"217","author":"He","year":"2018","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Diop, F., Vial, T., Ferraris, P., Wichit, S., Bengue, M., Hamel, R., Talignani, L., Liegeois, F., Pompon, J., and Yssel, H. (2018). Zika virus infection modulates the metabolomic profile of microglial cells. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0206093"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Russo, F.B., Jungmann, P., and Beltrao-Braga, P.C.B. (2017). Zika infection and the development of neurological defects. Cell Microbiol., 19.","DOI":"10.1111\/cmi.12744"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Bos, S., Viranaicken, W., Frumence, E., Li, G., Despres, P., Zhao, R.Y., and Gadea, G. (2019). The Envelope Residues E152\/156\/158 of Zika Virus Influence the Early Stages of Virus Infection in Human Cells. Cells, 8.","DOI":"10.3390\/cells8111444"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lunardelli, V.A.S., de Souza Apostolico, J., Souza, H.F.S., Coirada, F.C., Martinho, J.A., Astray, R.M., Boscardin, S.B., and Rosa, D.S. (2022). ZIKV-envelope proteins induce specific humoral and cellular immunity in distinct mice strains. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-20183-x"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.stem.2016.04.017","article-title":"Zika Virus Disrupts Neural Progenitor Development and Leads to Microcephaly in Mice","volume":"19","author":"Li","year":"2016","journal-title":"Cell Stem Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104987","DOI":"10.1016\/j.neuint.2021.104987","article-title":"Neuroendocrine, neuroinflammatory and pathological outcomes of chronic stress: A story of microglial remodeling","volume":"145","author":"Picard","year":"2021","journal-title":"Neurochem. Int."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Di Castro, M.A., Trettel, F., Milior, G., Maggi, L., Ragozzino, D., and Limatola, C. (2016). The chemokine CXCL16 modulates neurotransmitter release in hippocampal CA1 area. Sci. Rep., 6.","DOI":"10.1038\/srep34633"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1186\/s12974-022-02668-8","article-title":"Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response","volume":"19","author":"Manet","year":"2022","journal-title":"J. Neuroinflamm."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Chun, H., Marriott, I., Lee, C.J., and Cho, H. (2018). Elucidating the interactive roles of glia in Alzheimer\u2019s disease using established and newly developed experimental models. Front. Neurol., 9.","DOI":"10.3389\/fneur.2018.00797"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1126\/science.aad8373","article-title":"Complement and microglia mediate early synapse loss in Alzheimer mouse models","volume":"352","author":"Hong","year":"2016","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-4328(02)00272-3","article-title":"Glutamate receptor function in learning and memory","volume":"140","author":"Riedel","year":"2003","journal-title":"Behav. Brain Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1038\/ng.677","article-title":"Mutations in GRIN2A and GRIN2B encoding regulatory subunits of NMDA receptors cause variable neurodevelopmental phenotypes","volume":"42","author":"Endele","year":"2010","journal-title":"Nat. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.neuron.2017.02.014","article-title":"Single-cell profiling of an in vitro model of human interneuron development reveals temporal dynamics of cell type production and maturation","volume":"93","author":"Close","year":"2017","journal-title":"Neuron"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Tang, G.-B., Zeng, Y.-Q., Liu, P.-P., Mi, T.-W., Zhang, S.-F., Dai, S.-K., Tang, Q.-Y., Yang, L., Xu, Y.-J., and Yan, H.-L. (2017). The histone H3K27 demethylase UTX regulates synaptic plasticity and cognitive behaviors in mice. Front. Mol. Neurosci., 10.","DOI":"10.3389\/fnmol.2017.00267"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Govindpani, K., Turner, C., Waldvogel, H.J., Faull, R.L., and Kwakowsky, A. (2020). Impaired expression of GABA signaling components in the Alzheimer\u2019s disease middle temporal gyrus. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228704"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ibror.2020.07.005","article-title":"Overexpression of serotonin receptor 5b expression rescues neuronal and behavioral deficits in a mouse model of Kabuki syndrome","volume":"9","author":"Tang","year":"2020","journal-title":"IBRO Rep."},{"key":"ref_40","first-page":"190","article-title":"Role of gabra2, GABA(A) receptor alpha-2 subunit, in CNS development","volume":"3","year":"2015","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Smith, D.R., Hollidge, B., Daye, S., Zeng, X., Blancett, C., Kuszpit, K., Bocan, T., Koehler, J.W., Coyne, S., and Minogue, T. (2017). Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005296"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.nurt.2010.05.014","article-title":"Microglial activation and chronic neurodegeneration","volume":"7","author":"Lull","year":"2010","journal-title":"Neurotherapeutics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1177\/1352458514533230","article-title":"The role of microglial activation in disease progression","volume":"20","author":"Correale","year":"2014","journal-title":"Mult. Scler. J."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Dehmelt, L., and Halpain, S. (2005). The MAP2\/Tau family of microtubule-associated proteins. Genome Biol., 6.","DOI":"10.1186\/gb-2004-6-1-204"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.1093\/brain\/awx243","article-title":"Tau pathology and neurodegeneration contribute to cognitive impairment in Alzheimer\u2019s disease","volume":"140","author":"Bejanin","year":"2017","journal-title":"Brain"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1038\/nature11729","article-title":"NLRP3 is activated in Alzheimer\u2019s disease and contributes to pathology in APP\/PS1 mice","volume":"493","author":"Heneka","year":"2013","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1093\/brain\/awv081","article-title":"Reactive microglia drive tau pathology and contribute to the spreading of pathological tau in the brain","volume":"138","author":"Maphis","year":"2015","journal-title":"Brain"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"109720","DOI":"10.1016\/j.celrep.2021.109720","article-title":"Proteopathic tau primes and activates interleukin-1\u03b2 via myeloid-cell-specific MyD88-and NLRP3-ASC-inflammasome pathway","volume":"36","author":"Jiang","year":"2021","journal-title":"Cell Rep."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Granholm, A.-C. (2023). Long-term effects of SARS-CoV-2 in the brain: Clinical consequences and molecular mechanisms. J. Clin. Med., 12.","DOI":"10.3390\/jcm12093190"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/1040841X.2023.2190405","article-title":"Long-Term effects of COVID-19: A review of current perspectives and mechanistic insights","volume":"50","author":"Lenz","year":"2024","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Blackhurst, B.M., and Funk, K.E. (2023). Molecular and Cellular Mechanisms Underlying Neurologic Manifestations of Mosquito-Borne Flavivirus Infections. Viruses, 15.","DOI":"10.3390\/v15112200"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1038\/s41593-019-0427-y","article-title":"T cells promote microglia-mediated synaptic elimination and cognitive dysfunction during recovery from neuropathogenic flaviviruses","volume":"22","author":"Garber","year":"2019","journal-title":"Nat. Neurosci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1038\/nm.3232","article-title":"The role of autophagy in neurodegenerative disease","volume":"19","author":"Nixon","year":"2013","journal-title":"Nat. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1016\/j.neurobiolaging.2011.11.009","article-title":"Autophagic degradation of tau in primary neurons and its enhancement by trehalose","volume":"33","author":"Kruger","year":"2012","journal-title":"Neurobiol. Aging"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.4269\/ajtmh.16-0111","article-title":"Characterization of a novel murine model to study Zika virus","volume":"94","author":"Rossi","year":"2016","journal-title":"Am. J. Trop. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Nazerai, L., Pravsgaard Christensen, J., and Randrup Thomsen, A. (2019). A \u2018furry-tale\u2019of Zika virus infection: What have we learned from animal models?. Viruses, 11.","DOI":"10.3390\/v11010029"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/j.chom.2018.04.003","article-title":"An Immunocompetent Mouse Model of Zika Virus Infection","volume":"23","author":"Gorman","year":"2018","journal-title":"Cell Host Microbe"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9451\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:45:54Z","timestamp":1760111154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,30]]},"references-count":57,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ijms25179451"],"URL":"https:\/\/doi.org\/10.3390\/ijms25179451","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,30]]}}}