{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:58:58Z","timestamp":1791773938121,"version":"4.3.4"},"reference-count":62,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFA0806600"],"award-info":[{"award-number":["2022YFA0806600"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human mutations of ADNP and ADNP2 are known to be associated with neural developmental disorders (NDDs), including autism spectrum disorders (ASDs) and schizophrenia (SZ). However, the underlying mechanisms remain elusive. In this study, using CRISPR\/Cas9 gene editing technology, we generated adnp and adnp2 mutant zebrafish models, which exhibited developmental delays, brain deficits, and core behavioral features of NDDs. RNA sequencing analysis of adnpa\u2212\/\u2212; adnpb\u2212\/\u2212 and adnp2a\u2212\/\u2212; adnp2b\u2212\/\u2212 larval brains revealed altered gene expression profiles affecting synaptic transmission, autophagy, apoptosis, microtubule dynamics, hormone signaling, and circadian rhythm regulation. Validation using whole-mount in situ hybridization (WISH) and real-time quantitative PCR (qRT-PCR) corroborated these findings, supporting the RNA-seq results. Additionally, loss of adnp and adnp2 resulted in significant downregulation of pan-neuronal HuC and neuronal fiber network \u03b1-Tubulin signals. Importantly, prolonged low-dose exposure to environmental endocrine disruptors (EEDs) aggravated behavioral abnormalities in adnp and adnp2 mutants. This comprehensive approach enhances our understanding of the complex interplay between genetic mutations and environmental factors in NDDs. Our findings provide novel insights and experimental foundations into the roles of adnp and adnp2 in neurodevelopment and behavioral regulation, offering a framework for future preclinical drug screening aimed at elucidating the pathogenesis of NDDs and related conditions.<\/jats:p>","DOI":"10.3390\/ijms25179469","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T09:56:42Z","timestamp":1725357402000},"page":"9469","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genetic and Environmental Factors Co-Contributing to Behavioral Abnormalities in adnp\/adnp2 Mutant Zebrafish"],"prefix":"10.3390","volume":"25","author":[{"given":"Yongxin","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Sun","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2695-9100","authenticated-orcid":false,"given":"Bo","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0837-2450","authenticated-orcid":false,"given":"Ming","family":"Duan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"The Innovation of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7902-9022","authenticated-orcid":false,"given":"Yuhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"The Innovation of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"Hubei Hongshan Laboratory, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.jfda.2019.01.002","article-title":"Psychobiotics in mental health, neurodegenerative and neurodevelopmental disorders","volume":"27","author":"Cheng","year":"2019","journal-title":"J. Food Drug Anal."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"112922","DOI":"10.1016\/j.psychres.2020.112922","article-title":"Prevalence of comorbid psychiatric disorders among people with autism spectrum disorder: An umbrella review of systematic reviews and meta-analyses","volume":"287","author":"Hossain","year":"2020","journal-title":"Psychiatry Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S2215-0366(16)30376-5","article-title":"Neurodevelopmental disorders","volume":"4","author":"Thapar","year":"2017","journal-title":"Lancet Psychiatry"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.schres.2013.05.028","article-title":"Definition and description of schizophrenia in the DSM-5","volume":"150","author":"Tandon","year":"2013","journal-title":"Schizophr. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1002\/jnr.23808","article-title":"Sexual divergence in activity-dependent neuroprotective protein impacting autism, schizophrenia, and Alzheimer\u2019s disease","volume":"95","author":"Gozes","year":"2017","journal-title":"J. Neurosci. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112867","DOI":"10.1016\/j.celrep.2023.112867","article-title":"POGZ suppresses 2C transcriptional program and retrotransposable elements","volume":"42","author":"Sun","year":"2023","journal-title":"Cell Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13229-022-00502-9","article-title":"Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF","volume":"13","author":"Sun","year":"2022","journal-title":"Mol. Autism"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2984","DOI":"10.1038\/s41467-020-16799-0","article-title":"ADNP promotes neural differentiation by modulating Wnt\/\u03b2-catenin signaling","volume":"11","author":"Sun","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Bainomugisa, C.K., Sutherland, H.G., Parker, R., McRae, A.F., Haupt, L.M., Griffiths, L.R., Heath, A., Nelson, E.C., Wright, M.J., and Hickie, I.B. (2021). Using Monozygotic Twins to Dissect Common Genes in Posttraumatic Stress Disorder and Migraine. Front. Neurosci., 15.","DOI":"10.3389\/fnins.2021.678350"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1111\/j.1471-4159.2007.05173.x","article-title":"Silencing of the ADNP-family member, ADNP2, results in changes in cellular viability under oxidative stress","volume":"105","author":"Kushnir","year":"2008","journal-title":"J. Neurochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1038\/s41586-022-04434-5","article-title":"Mapping genomic loci implicates genes and synaptic biology in schizophrenia","volume":"604","author":"Trubetskoy","year":"2022","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.euroneuro.2010.06.004","article-title":"Activity-dependent neuroprotective protein (ADNP) expression level is correlated with the expression of the sister protein ADNP2: Deregulation in schizophrenia","volume":"21","author":"Dresner","year":"2011","journal-title":"Eur. Neuropsychopharmacol."},{"key":"ref_13","first-page":"157","article-title":"Genome-wide copy number microarray analysis for a boy with autism","volume":"36","author":"He","year":"2019","journal-title":"Zhonghua Yi Xue Yi Chuan Xue Za Zhi"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/s41398-020-01106-8","article-title":"Autism-associated miR-873 regulates ARID1B, SHANK3 and NRXN2 involved in neurodevelopment","volume":"10","author":"Lu","year":"2020","journal-title":"Transl. Psychiatry"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1749-6632.1999.tb07884.x","article-title":"A novel signaling molecule for neuropeptide action: Activity-dependent neuroprotective protein","volume":"897","author":"Gozes","year":"1999","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1074\/jbc.M007416200","article-title":"Cloning and characterization of the human activity-dependent neuroprotective protein","volume":"276","author":"Zamostiano","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0165-3806(03)00162-7","article-title":"Activity-dependent neuroprotective protein: A novel gene essential for brain formation","volume":"144","author":"Pinhasov","year":"2003","journal-title":"Brain Res. Dev. Brain Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1111\/j.1471-4159.2006.03936.x","article-title":"Peptide neuroprotection through specific interaction with brain tubulin","volume":"98","author":"Divinski","year":"2006","journal-title":"J. Neurochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"40173","DOI":"10.1074\/jbc.M112.387027","article-title":"Novel evolutionary-conserved role for the activity-dependent neuroprotective protein (ADNP) family that is important for erythropoiesis","volume":"287","author":"Dresner","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1124\/jpet.107.129551","article-title":"Activity-dependent neuroprotective protein snippet NAP reduces tau hyperphosphorylation and enhances learning in a novel transgenic mouse model","volume":"323","author":"Pinhasov","year":"2007","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4956","DOI":"10.1172\/JCI98199","article-title":"Activity-dependent neuroprotective protein deficiency models synaptic and developmental phenotypes of autism-like syndrome","volume":"128","author":"Sragovich","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"800","DOI":"10.24272\/j.issn.2095-8137.2021.251","article-title":"Understanding autism spectrum disorders with animal models: Applications, insights, and perspectives","volume":"42","author":"Li","year":"2021","journal-title":"Zool. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s13229-022-00516-3","article-title":"Disruption of grin2B, an ASD-associated gene, produces social deficits in zebrafish","volume":"13","author":"Zoodsma","year":"2022","journal-title":"Mol. Autism"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s13229-018-0204-x","article-title":"CRISPR\/Cas9-induced shank3b mutant zebrafish display autism-like behaviors","volume":"9","author":"Liu","year":"2018","journal-title":"Mol. Autism"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Doi, M., Usui, N., and Shimada, S. (2022). Prenatal Environment and Neurodevelopmental Disorders. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.860110"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19770","DOI":"10.1074\/jbc.M115.665729","article-title":"Gene-Environment Interactions Target Mitogen-activated Protein 3 Kinase 1 (MAP3K1) Signaling in Eyelid Morphogenesis","volume":"290","author":"Mongan","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ijomone, O.M., Olung, N.F., Akingbade, G.T., Okoh, C.O.A., and Aschner, M. (2020). Environmental influence on neurodevelopmental disorders: Potential association of heavy metal exposure and autism. J. Trace Elem. Med. Biol., 62.","DOI":"10.1016\/j.jtemb.2020.126638"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100736","DOI":"10.1016\/j.xcrm.2022.100736","article-title":"Gene-environment interactions explain a substantial portion of variability of common neuropsychiatric disorders","volume":"3","author":"Zhang","year":"2022","journal-title":"Cell Rep. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3725","DOI":"10.1016\/j.fct.2012.07.059","article-title":"A review of dietary and non-dietary exposure to bisphenol-A","volume":"50","author":"Geens","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.envres.2017.11.007","article-title":"A prospective cohort study of the association between bisphenol A exposure and the serum levels of liver enzymes in children","volume":"161","author":"Lee","year":"2018","journal-title":"Environ. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s00244-008-9194-6","article-title":"Studies on the toxicological effects of PFOA and PFOS on rats using histological observation and chemical analysis","volume":"56","author":"Cui","year":"2009","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1002\/etc.4815","article-title":"Per- and Polyfluoroalkyl Substances (PFAS) in Fish from European Lakes: Current Contamination Status, Sources, and Perspectives for Monitoring","volume":"40","author":"Valsecchi","year":"2021","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.aquatox.2012.04.003","article-title":"Long-term effects of a binary mixture of perfluorooctane sulfonate (PFOS) and bisphenol A (BPA) in zebrafish (Danio rerio)","volume":"118\u2013119","author":"Keiter","year":"2012","journal-title":"Aquat. Toxicol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1073\/pnas.1417731112","article-title":"Low-dose exposure to bisphenol A and replacement bisphenol S induces precocious hypothalamic neurogenesis in embryonic zebrafish","volume":"112","author":"Kinch","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tukker, A.M., Bouwman, L.M.S., van Kleef, R., Hendriks, H.S., Legler, J., and Westerink, R.H.S. (2020). Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human \u03b1(1)\u03b2(2)\u03b3(2L) GABA(A) receptor and spontaneous neuronal network function in vitro. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-62152-2"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1038\/s41380-019-0454-9","article-title":"Foetal oestrogens and autism","volume":"25","author":"Tsompanidis","year":"2020","journal-title":"Mol. Psychiatry"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/s41586-018-0153-8","article-title":"Activity-dependent neuroprotective protein recruits HP1 and CHD4 to control lineage-specifying genes","volume":"557","author":"Ostapcuk","year":"2018","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1210\/en.2016-1776","article-title":"GABAergic Neurons and Their Modulatory Effects on GnRH3 in Zebrafish","volume":"158","author":"Song","year":"2017","journal-title":"Endocrinology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/s41398-018-0357-6","article-title":"The autism\/neuroprotection-linked ADNP\/NAP regulate the excitatory glutamatergic synapse","volume":"9","author":"Sragovich","year":"2019","journal-title":"Transl. Psychiatry"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2655","DOI":"10.1523\/JNEUROSCI.23-07-02655.2003","article-title":"Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)","volume":"23","author":"Stepanova","year":"2003","journal-title":"J. Neurosci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1038\/mp.2014.97","article-title":"The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins","volume":"19","author":"Oz","year":"2014","journal-title":"Mol. Psychiatry"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/sj.onc.1203308","article-title":"EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue","volume":"19","author":"Nakagawa","year":"2000","journal-title":"Oncogene"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1038\/s41380-019-0563-5","article-title":"Discovery of autism\/intellectual disability somatic mutations in Alzheimer\u2019s brains: Mutated ADNP cytoskeletal impairments and repair as a case study","volume":"26","author":"Hadar","year":"2021","journal-title":"Mol. Psychiatry"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1038\/s41380-018-0198-y","article-title":"Sex-specific impact of prenatal androgens on social brain default mode subsystems","volume":"25","author":"Lombardo","year":"2020","journal-title":"Mol. Psychiatry"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/s41398-020-00904-4","article-title":"Tauopathy in the young autistic brain: Novel biomarker and therapeutic target","volume":"10","author":"Grigg","year":"2020","journal-title":"Transl. Psychiatry"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1002\/cm.21776","article-title":"Tau, ADNP, and sex","volume":"81","author":"Gozes","year":"2024","journal-title":"Cytoskeleton"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.biopsych.2021.09.018","article-title":"Novel ADNP Syndrome Mice Reveal Dramatic Sex-Specific Peripheral Gene Expression With Brain Synaptic and Tau Pathologies","volume":"92","author":"Karmon","year":"2022","journal-title":"Biol. Psychiatry"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1038\/s41380-022-01939-3","article-title":"The cytoplasmic localization of ADNP through 14-3-3 promotes sex-dependent neuronal morphogenesis, cortical connectivity, and calcium signaling","volume":"28","author":"Bennison","year":"2023","journal-title":"Mol. Psychiatry"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e3273","DOI":"10.1002\/brb3.3273","article-title":"Circadian clock crosstalks with autism","volume":"13","author":"Yurdakul","year":"2023","journal-title":"Brain Behav."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lorsung, E., Karthikeyan, R., and Cao, R. (2021). Biological Timing and Neurodevelopmental Disorders: A Role for Circadian Dysfunction in Autism Spectrum Disorders. Front. Neurosci., 15.","DOI":"10.3389\/fnins.2021.642745"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1093\/hmg\/ddn380","article-title":"Reciprocal co-regulation of EGR2 and MECP2 is disrupted in Rett syndrome and autism","volume":"18","author":"Swanberg","year":"2009","journal-title":"Hum. Mol. Genet."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0166-4328(02)00421-7","article-title":"How necessary is the activation of the immediate early gene zif268 in synaptic plasticity and learning?","volume":"142","author":"Davis","year":"2003","journal-title":"Behav. Brain Res."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Colucci-D\u2019Amato, L., Speranza, L., and Volpicelli, F. (2020). Neurotrophic Factor BDNF, Physiological Functions and Therapeutic Potential in Depression, Neurodegeneration and Brain Cancer. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207777"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Andreska, T., Aufmkolk, S., Sauer, M., and Blum, R. (2014). High abundance of BDNF within glutamatergic presynapses of cultured hippocampal neurons. Front. Cell. Neurosci., 8.","DOI":"10.3389\/fncel.2014.00107"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Sragovich, S., Merenlender-Wagner, A., and Gozes, I. (2017). ADNP Plays a Key Role in Autophagy: From Autism to Schizophrenia and Alzheimer\u2019s Disease. Bioessays, 39.","DOI":"10.1002\/bies.201700054"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12031-007-9013-y","article-title":"ADNP differential nucleus\/cytoplasm localization in neurons suggests multiple roles in neuronal differentiation and maintenance","volume":"35","author":"Mandel","year":"2008","journal-title":"J. Mol. Neurosci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.yfrne.2012.08.005","article-title":"The cerebellum as a target for estrogen action","volume":"33","author":"Hedges","year":"2012","journal-title":"Front. Neuroendocrinol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/jcpp.12501","article-title":"Annual Research Review: The role of the environment in the developmental psychopathology of autism spectrum condition","volume":"57","author":"Mandy","year":"2016","journal-title":"J. Child Psychol. Psychiatry"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.envint.2018.02.014","article-title":"Epigenetics as a mechanism linking developmental exposures to long-term toxicity","volume":"114","author":"Barouki","year":"2018","journal-title":"Environ. Int."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zheng, J., Long, F., Cao, X., Xiong, B., and Li, Y. (2022). Knockout of Katnal2 Leads to Autism-like Behaviors and Developmental Delay in Zebrafish. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23158389"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"157816","DOI":"10.1016\/j.scitotenv.2022.157816","article-title":"Perfluorooctane sulfonic acid (PFOS) exposures interfere with behaviors and transcription of genes on nervous and muscle system in zebrafish embryos","volume":"848","author":"Wang","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s10695-023-01284-4","article-title":"BPA induces hepatotoxicity in zebrafish through oxidative stress and apoptosis pathways","volume":"50","author":"Wang","year":"2024","journal-title":"Fish Physiol. Biochem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9469\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:46:04Z","timestamp":1760111164000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9469"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,30]]},"references-count":62,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ijms25179469"],"URL":"https:\/\/doi.org\/10.3390\/ijms25179469","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,30]]}}}