{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:27:05Z","timestamp":1791343625695,"version":"4.3.1"},"reference-count":60,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T00:00:00Z","timestamp":1725062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science &amp; Technology Innovation Talents in Universities of Henan Province","award":["21HASTIT035"],"award-info":[{"award-number":["21HASTIT035"]}]},{"name":"Science &amp; Technology Innovation Talents in Universities of Henan Province","award":["2101102A"],"award-info":[{"award-number":["2101102A"]}]},{"name":"Science &amp; Technology Innovation Talents in Universities of Henan Province","award":["(2021)44"],"award-info":[{"award-number":["(2021)44"]}]},{"name":"Luoyang City Science and Technology Planning","award":["21HASTIT035"],"award-info":[{"award-number":["21HASTIT035"]}]},{"name":"Luoyang City Science and Technology Planning","award":["2101102A"],"award-info":[{"award-number":["2101102A"]}]},{"name":"Luoyang City Science and Technology Planning","award":["(2021)44"],"award-info":[{"award-number":["(2021)44"]}]},{"name":"Top Young Talents in Central Plains","award":["21HASTIT035"],"award-info":[{"award-number":["21HASTIT035"]}]},{"name":"Top Young Talents in Central Plains","award":["2101102A"],"award-info":[{"award-number":["2101102A"]}]},{"name":"Top Young Talents in Central Plains","award":["(2021)44"],"award-info":[{"award-number":["(2021)44"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sulfur dioxide (SO2) is the most effective preservative for table grapes as it reduces the respiratory intensity of berries and inhibits mold growth. However, excessive SO2 causes berry abscission during storage, resulting in an economic loss postharvest. In this study, grapes were exogenously treated with SO2, SO2 + 1.5% chitosan, SO2 + 1.5% eugenol, and SO2 + eugenol-loaded chitosan nanoparticles (SN). In comparison to SO2 treatment, SN treatment reduced the berries\u2019 abscission rate by 74% while maintaining the quality of the berries. Among the treatments, SN treatment most effectively inhibited berry abscission and maintained berry quality. RNA-sequencing (RNA-seq) revealed that SN treatment promoted the expression of genes related to cell wall metabolism. Among these genes, VlCOMT was detected as the central gene, playing a key role in mediating the effects of SN. Dual luciferase and yeast one-hybrid (Y1H) assays demonstrated that VlbZIP14 directly activated VlCOMT by binding to the G-box motif in the latter\u2019s promoter, which then participated in lignin synthesis. Our results provide key insights into the molecular mechanisms underlying the SN-mediated inhibition of berry abscission and could be used to improve the commercial value of SO2-treated postharvest table grapes.<\/jats:p>","DOI":"10.3390\/ijms25179479","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T09:56:42Z","timestamp":1725357402000},"page":"9479","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Transcription Factor VlbZIP14 Inhibits Postharvest Grape Berry Abscission by Directly Activating VlCOMT and Promoting Lignin Biosynthesis"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6396-2272","authenticated-orcid":false,"given":"Peng","family":"Yu","sequence":"first","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songqi","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadan","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxuan","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaofang","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7240-7975","authenticated-orcid":false,"given":"Yihe","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.plaphy.2019.09.010","article-title":"Grape (Vitis vinifera) VvDOF3 Functions as a Transcription Activator and Enhances Powdery Mildew Resistance","volume":"143","author":"Yu","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1093\/pcp\/pcaa118","article-title":"Grapevine U-Box E3 Ubiquitin Ligase VlPUB38 Negatively Regulates Fruit Ripening by Facilitating Abscisic-Aldehyde Oxidase Degradation","volume":"61","author":"Yu","year":"2020","journal-title":"Plant Cell Physiol."},{"key":"ref_3","unstructured":"(2024, August 28). Home|OIV. Available online: https:\/\/www.oiv.int\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.foodchem.2007.06.012","article-title":"Physiological Responses and Quality Attributes of Table Grape Fruit to Chitosan Preharvest Spray and Postharvest Coating during Storage","volume":"106","author":"Meng","year":"2008","journal-title":"Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, M., Huang, Z., You, X., Zhang, Y., Wei, P., Zhou, K., and Wang, Y. (2020). Relationships between Cell Structure Alterations and Berry Abscission in Table Grapes. Front. Nutr., 7.","DOI":"10.3389\/fnut.2020.00069"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2396","DOI":"10.1093\/plphys\/kiac212","article-title":"The HD-Zip Transcription Factor SlHB15A Regulates Abscission by Modulating Jasmonoyl-Isoleucine Biosynthesis","volume":"189","author":"Liu","year":"2022","journal-title":"Plant Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1093\/jxb\/eraa162","article-title":"KNOX Protein KNAT1 Regulates Fruitlet Abscission in Litchi by Repressing Ethylene Biosynthetic Genes","volume":"71","author":"Zhao","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/s41438-021-00540-z","article-title":"Brassinosteroids Suppress Ethylene-Induced Fruitlet Abscission through LcBZR1\/2-Mediated Transcriptional Repression of LcACS1\/4 and LcACO2\/3 in Litchi","volume":"8","author":"Ma","year":"2021","journal-title":"Hortic. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3485","DOI":"10.1105\/tpc.19.00695","article-title":"Auxin Regulates Sucrose Transport to Repress Petal Abscission in Rose (Rosa hybrida)","volume":"32","author":"Liang","year":"2020","journal-title":"Plant Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4388","DOI":"10.1093\/plcell\/koac254","article-title":"A SlCLV3-SlWUS Module Regulates Auxin and Ethylene Homeostasis in Low Light-Induced Tomato Flower Abscission","volume":"34","author":"Cheng","year":"2022","journal-title":"Plant Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.postharvbio.2019.02.012","article-title":"Edible Coatings Inhibit the Postharvest Berry Abscission of Table Grapes Caused by Sulfur Dioxide during Storage","volume":"152","author":"Chen","year":"2019","journal-title":"Postharvest Biol. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S1671-2927(09)60009-2","article-title":"Effects of ABA Content on the Development of Abscission Zone and Berry Falling after Harvesting of Grapes","volume":"8","author":"Zhang","year":"2009","journal-title":"Agric. Sci. China"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"504","DOI":"10.5344\/ajev.2014.14038","article-title":"Methyl Jasmonate and 1-Aminocyclopropane-1-Carboxylic Acid Interact to Promote Grape Berry Abscission","volume":"65","author":"Uzquiza","year":"2014","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wu, P., Xin, F., Xu, H., Chu, Y., Du, Y., Tian, H., and Zhu, B. (2021). Chitosan Inhibits Postharvest Berry Abscission of \u2018Kyoho\u2019 Table Grapes by Affecting the Structure of Abscission Zone, Cell Wall Degrading Enzymes and SO2 Permeation. Postharvest Biol. Technol., 176.","DOI":"10.1016\/j.postharvbio.2021.111507"},{"key":"ref_15","first-page":"133","article-title":"The Relation of Grape Berry Abscission to Changes of Enzyme Activit in Abscission Zone and Plant Growth Regulators during Storag","volume":"29","author":"Chen","year":"2003","journal-title":"Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = J. Plant Physiol. Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111280","DOI":"10.1016\/j.scienta.2022.111280","article-title":"Susceptibility of Two Grape Varieties to Berry Abscission","volume":"304","author":"Zhu","year":"2022","journal-title":"Sci. Hortic."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1038\/s41438-020-00385-y","article-title":"A Genome-Wide Association Study Uncovers a Critical Role of the RsPAP2 Gene in Red-Skinned Raphanus sativus L","volume":"7","author":"Fan","year":"2020","journal-title":"Hortic. Res."},{"key":"ref_18","first-page":"28","article-title":"The Relationship Between Fruit Structure and Storability in Table Grapes","volume":"01","author":"Zhou","year":"2006","journal-title":"Acta Horticulturae Sinica."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.postharvbio.2008.03.006","article-title":"Extended Storage of \u2018Red Globe\u2019 Grapes in Modified SO2 Generating Pads","volume":"50","author":"Zutahy","year":"2008","journal-title":"Postharvest Biol. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"919","DOI":"10.21273\/HORTSCI.31.6.919","article-title":"Alternatives to Sulfur Dioxide Fumigation for Table Grape Storage","volume":"31","author":"Ahmedullah","year":"1996","journal-title":"HortScience"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1080\/13102818.2022.2027816","article-title":"Antimicrobial Activity of Chitosan Nanoparticles","volume":"35","author":"Bora","year":"2021","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1089\/jmf.2010.0197","article-title":"Antioxidant Activity of Eugenol: A Structure\u2013Activity Relationship Study","volume":"14","year":"2011","journal-title":"J. Med. Food."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1105\/tpc.13.1.73","article-title":"Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin","volume":"13","author":"Guo","year":"2001","journal-title":"Plant Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1016\/j.msec.2013.01.010","article-title":"Chitosan Based Edible Films and Coatings: A Review","volume":"33","author":"Elsabee","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1080\/15538362.2016.1219290","article-title":"Strawberry Shelf Life, Composition, and Enzymes Activity in Response to Edible Chitosan Coatings","volume":"17","author":"Badawy","year":"2017","journal-title":"Int. J. Fruit Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.postharvbio.2004.01.008","article-title":"Edible Coatings to Improve Storability and Enhance Nutritional Value of Fresh and Frozen Strawberries (Fragaria \u00d7 Ananassa) and Raspberries (Rubus ideaus)","volume":"33","author":"Han","year":"2004","journal-title":"Postharvest Biol. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Nguyen, V.T.B., Nguyen, D.H.H., and Nguyen, H.V.H. (2020). Combination Effects of Calcium Chloride and Nano-Chitosan on the Postharvest Quality of Strawberry (Fragaria x Ananassa Duch.). Postharvest Biol. Technol., 162.","DOI":"10.1016\/j.postharvbio.2019.111103"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"575","DOI":"10.21273\/HORTSCI.35.4.575","article-title":"The Role of Lipid Peroxidation in Storage Disorders of Fresh Fruits and Vegetables","volume":"35","author":"Shewfelt","year":"2000","journal-title":"HortScience"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.scienta.2018.11.006","article-title":"The Variation of Berry Development and DNA Methylation after Treatment with 5-azaC on \u2018Kyoho\u2019 Grape","volume":"246","author":"Guo","year":"2019","journal-title":"Sci. Hortic."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Fenech, M., Amaya, I., Valpuesta, V., and Botella, M.A. (2019). Vitamin C Content in Fruits: Biosynthesis and Regulation. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.02006"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.foodchem.2018.12.078","article-title":"Effects of Graft Copolymer of Chitosan and Salicylic Acid on Reducing Rot of Postharvest Fruit and Retarding Cell Wall Degradation in Grapefruit during Storage","volume":"283","author":"Shi","year":"2019","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.postharvbio.2016.03.002","article-title":"van Increased Hydrolase Gene Expression and Hydrolase Activity in the Abscission Zone Involved in Chilling-Induced Abscission of Dendrobium Flowers","volume":"117","author":"Phetsirikoon","year":"2016","journal-title":"Postharvest Biol. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.postharvbio.2006.07.013","article-title":"Studies of Postharvest Berry Abscission of \u2018Kyoho\u2019 Table Grapes during Cold Storage and High Oxygen Atmospheres","volume":"43","author":"Deng","year":"2007","journal-title":"Postharvest Biol. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1111\/tpj.15201","article-title":"LcERF2 Modulates Cell Wall Metabolism by Directly Targeting a UDP-Glucose-4-Epimerase Gene to Regulate Pedicel Development and Fruit Abscission of Litchi","volume":"106","author":"Yi","year":"2021","journal-title":"Plant J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1093\/mp\/ssp106","article-title":"Phenylpropanoid Biosynthesis","volume":"3","author":"Vogt","year":"2010","journal-title":"Mol. Plant"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Qiu, Z., Wen, Z., Hou, Q., Qiao, G., Yang, K., Hong, Y., and Wen, X. (2021). Cross-Talk between Transcriptome, Phytohormone and HD-ZIP Gene Family Analysis Illuminates the Molecular Mechanism Underlying Fruitlet Abscission in Sweet Cherry (Prunus avium L). BMC Plant Biol., 21.","DOI":"10.1186\/s12870-021-02940-8"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.molp.2019.05.008","article-title":"Phosphorylation of LTF1, an MYB Transcription Factor in Populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells","volume":"12","author":"Gui","year":"2019","journal-title":"Mol. Plant."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/s41438-020-00451-5","article-title":"Lignin Provides Mechanical Support to Herbaceous Peony (Paeonia lactiflora Pall.) Stems","volume":"7","author":"Zhao","year":"2020","journal-title":"Hortic. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.cj.2021.09.005","article-title":"A Lignified-Layer Bridge Controlled by a Single Recessive Gene Is Associated with High Pod-Shatter Resistance in Brassica napus L","volume":"10","author":"Chu","year":"2022","journal-title":"Crop J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1105\/tpc.18.00304","article-title":"The APETALA2-like Transcription Factor SUPERNUMERARY BRACT Controls Rice Seed Shattering and Seed Size","volume":"31","author":"Jiang","year":"2019","journal-title":"Plant Cell"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Liu, J., Chen, N., Chen, F., Cai, B., Dal Santo, S., Tornielli, G.B., Pezzotti, M., and Cheng, Z.-M. (2014). Genome-Wide Analysis and Expression Profile of the bZIP Transcription Factor Gene Family in Grapevine (Vitis vinifera). BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-281"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1096\/fasebj.8.2.8119490","article-title":"Plant bZIP Proteins Gather at ACGT Elements","volume":"8","author":"Foster","year":"1994","journal-title":"FASEB J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s11103-008-9298-4","article-title":"A bZIP Transcription Factor, OsABI5, Is Involved in Rice Fertility and Stress Tolerance","volume":"66","author":"Zou","year":"2008","journal-title":"Plant Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.carbpol.2012.08.117","article-title":"Eugenol-Loaded Chitosan Nanoparticles: I. Thermal Stability Improvement of Eugenol through Encapsulation","volume":"96","author":"Woranuch","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.nbt.2016.07.015","article-title":"Linking Hormonal Profiles with Variations in Sugar and Anthocyanin Contents during the Natural Development and Ripening of Sweet Cherries","volume":"33","author":"Teribia","year":"2016","journal-title":"New Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.hpj.2017.07.004","article-title":"Effect of Storage Temperature on Vitamin C, Total Phenolics, UPLC Phenolic Acid Profile and Antioxidant Capacity of Eleven Potato (Solanum tuberosum) Varieties","volume":"3","author":"Mankad","year":"2017","journal-title":"Hortic. Plant J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.postharvbio.2011.07.001","article-title":"Effects of a Phospholipase D Inhibitor on Postharvest Enzymatic Browning and Oxidative Stress of Litchi Fruit","volume":"62","author":"Sun","year":"2011","journal-title":"Postharvest Biol. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.postharvbio.2014.09.001","article-title":"Postharvest ASM Dipping and DPI Pre-Treatment Regulated Reactive Oxygen Species Metabolism in Muskmelon (Cucumis melo L.) Fruit","volume":"99","author":"Ge","year":"2015","journal-title":"Postharvest Biol. Technol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e5580","DOI":"10.7717\/peerj.5580","article-title":"Cell-Wall-Degrading Enzymes Produced in Vitro and in Vivo by Rhizoctonia Solani, the Causative Fungus of Peanut Sheath Blight","volume":"6","author":"Xue","year":"2018","journal-title":"PeerJ"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.foodres.2005.03.003","article-title":"Changes in Firmness, Cell Wall Composition and Cell Wall Hydrolases of Grapes Stored in High Oxygen Atmospheres","volume":"38","author":"Deng","year":"2005","journal-title":"Food Res. Int."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9162","DOI":"10.1073\/pnas.1633612100","article-title":"The Lipoxygenase Gene ALOXE3 Implicated in Skin Differentiation Encodes a Hydroperoxide Isomerase","volume":"100","author":"Yu","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Li, B., and Dewey, C.N. (2011). RSEM: Accurate Transcript Quantification from RNA-Seq Data with or without a Reference Genome. BMC Bioinf., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Klopfenstein, D.V., Zhang, L., Pedersen, B.S., Ram\u00edrez, F., Warwick Vesztrocy, A., Naldi, A., Mungall, C.J., Yunes, J.M., Botvinnik, O., and Weigel, M. (2018). GOATOOLS: A Python Library for Gene Ontology Analyses. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-28948-z"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Langfelder, P., and Horvath, S. (2008). WGCNA: An R Package for Weighted Correlation Network Analysis. BMC Bioinf., 9.","DOI":"10.1186\/1471-2105-9-559"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing Real-Time PCR Data by the Comparative CT Method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_57","unstructured":"Liu, S. (2022). Determination of Chemical Components in Flax Fibers. [Master\u2019s Thesis, Donghua University]."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.biombioe.2015.10.007","article-title":"Comparison of Lignin, Cellulose, and Hemicellulose Contents for Biofuels Utilization among 4 Types of Lignocellulosic Crops","volume":"83","author":"Jung","year":"2015","journal-title":"Biomass Bioenergy"},{"key":"ref_59","unstructured":"\u017d\u00e1rsk\u00fd, V., and Cvr\u010dkov\u00e1, F. (2014). Identifying Subcellular Protein Localization with Fluorescent Protein Fusions after Transient Expression in Onion Epidermal Cells. Plant Cell Morphogenesis: Methods and Protocols, Humana Press."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1111\/nph.13857","article-title":"Plant VAP27 Proteins: Domain Characterization, Intracellular Localization and Role in Plant Development","volume":"210","author":"Wang","year":"2016","journal-title":"New Phytol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9479\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:46:10Z","timestamp":1760111170000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,31]]},"references-count":60,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ijms25179479"],"URL":"https:\/\/doi.org\/10.3390\/ijms25179479","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,31]]}}}