{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:45:27Z","timestamp":1791819927597,"version":"4.3.4"},"reference-count":192,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T00:00:00Z","timestamp":1725062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon Europe research and innovation program under the Marie Sklodowska Curie","award":["101109357"],"award-info":[{"award-number":["101109357"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Nanotechnology has gained popularity in recent years due to its wide-ranging applications within the scientific community. The three main methods for synthesizing nanoparticles are physical, chemical, and biological. However, the adverse effects associated with physical and chemical methods have led to a growing interest in biological methods. Interestingly, green synthesis using plants has gained prominence in developing new treatments for bacterial infections. Zinc oxide nanoparticles (ZnO NPs) produced using environmentally friendly methods are more biocompatible and have potential applications as antibacterial agents in the biomedical field. As a result, this review discusses the green synthesis of ZnO NPs, factors influencing optimal synthesis, characterization techniques, and the antibacterial activity of some plant-mediated ZnO NPs. It also provides a comprehensive and analytical exploration of ZnO NP biosynthesis, the role of phytochemical compounds as reducing and stabilizing agents, the mechanism of action of their antibacterial properties and further highlights the challenges and prospects in this innovative research area.<\/jats:p>","DOI":"10.3390\/ijms25179500","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T12:22:33Z","timestamp":1725366153000},"page":"9500","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Biogenic Zinc Oxide Nanoparticles as a Promising Antibacterial Agent: Synthesis and Characterization"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7211-714X","authenticated-orcid":false,"given":"Kunle","family":"Okaiyeto","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of \u201cG. d\u2019Annunzio\u201d of Chieti and Pescara, Via dei Vestini, 1, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosa","family":"Gigliobianco","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of \u201cG. d\u2019Annunzio\u201d of Chieti and Pescara, Via dei Vestini, 1, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7657-3556","authenticated-orcid":false,"given":"Piera","family":"Di Martino","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of \u201cG. d\u2019Annunzio\u201d of Chieti and Pescara, Via dei Vestini, 1, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Karam, S.T., and Abdulrahman, A.F. 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