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Immune cell ratios, like monocyte\/lymphocyte ratio (MLR) and neutrophil\/lymphocyte ratio (NLR), offer a more comprehensive view of immune status compared to individual cell counts. However, research on pig immune cell ratios remains limited. This study investigated MLR and NLR in a Duroc \u00d7 Erhualian F2 resource population. Heritability analysis revealed high values (0.649 and 0.688 for MLR and NLR, respectively), suggesting a strong genetic component. Furthermore, we employed an ensemble-like GWAS (E-GWAS) strategy and functional annotation analysis to identify 11 MLR-associated and 6 NLR-associated candidate genes. These genes were significantly enriched in immune-related biological processes. These findings provide novel genetic markers and candidate genes associated with porcine immunity, thereby providing valuable insights for addressing biosecurity and animal welfare concerns in the pig industry.<\/jats:p>","DOI":"10.3390\/ijms25179745","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T09:21:00Z","timestamp":1725873660000},"page":"9745","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of SNPs and Candidate Genes Associated with Monocyte\/Lymphocyte Ratio and Neutrophil\/Lymphocyte Ratio in Duroc \u00d7 Erhualian F2 Population"],"prefix":"10.3390","volume":"25","author":[{"given":"Jiakun","family":"Qiao","sequence":"first","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4039-2156","authenticated-orcid":false,"given":"Minghang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangjun","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5447-4774","authenticated-orcid":false,"given":"Zhaoxuan","family":"Che","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-4312","authenticated-orcid":false,"given":"Pingping","family":"Han","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Miao","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8931-5022","authenticated-orcid":false,"given":"Mengjin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1016\/S0140-6736(00)04904-7","article-title":"An overview of the immune system","volume":"357","author":"Parkin","year":"2001","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4939-1115-8_1","article-title":"A brief outline of the immune system","volume":"1184","author":"Tomar","year":"2014","journal-title":"Methods Mol. 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