{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:05:56Z","timestamp":1791925556322,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:00:00Z","timestamp":1726790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Petr Shegay","award":["125284"],"award-info":[{"award-number":["125284"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Radiotherapy is one of the risk factors for radiation-induced premature ovarian failure and infertility in cancer patients. The development of methods for ovarian radioprotection remains relevant. Moreover, electrons are a little-studied and promising method of radiation with the least toxic effect on normal tissues. The assessment of intracellular mechanisms regulating the protective effects of leukocyte-poor platelet-rich plasma in a model of radiation-induced premature ovarian failure caused by electron irradiation. Wistar rats were divided into four groups, namely a control group, irradiation group (electron exposure), irradiation + leukocyte-poor platelet-rich plasma group, and only leukocyte-poor platelet-rich plasma group. Fragments of ovaries were removed and hormonal, oxidant, histological, and morphometric studies were carried out. The cell cycle of ovarian follicles and the inflammatory and vascular response were assessed using immunohistochemistry. The activity of MAPK, ERK, and PI3K pathways was also assessed using the RT-qPCR. We found that electron irradiation causes a decrease in the functional activity of the ovaries and the death of follicular cells through apoptosis. The administration of LP-PRP led to a partial restoration of the cytokine balance. In addition, minor ovarian damage and mild inflammation were observed in this group. Leukocyte-poor platelet-rich plasma components have anti-inflammatory, angiogenetic, and radioprotective effects, reducing the activation of the NOX4, caspase and cytokine cascades, and inflammatory response severity through the MAPK\/p38\/JNK signaling pathway. This leads to the induction of endogenous antioxidant protection, the repair of post-radiation follicular damage, and slowing down the development of radiation-induced premature ovarian failure after electron irradiation.<\/jats:p>","DOI":"10.3390\/ijms251810115","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T07:38:57Z","timestamp":1726817937000},"page":"10115","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Novel Molecular Mechanisms Underlying the Ameliorative Effect of Platelet-Rich Plasma against Electron Radiation-Induced Premature Ovarian Failure"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8447-2600","authenticated-orcid":false,"given":"Grigory","family":"Demyashkin","sequence":"first","affiliation":[{"name":"Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia"},{"name":"Laboratory of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology, Sechenov University, Trubetskaya St., 8\/2, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6235-1020","authenticated-orcid":false,"given":"Matvey","family":"Vadyukhin","sequence":"additional","affiliation":[{"name":"Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaira","family":"Murtazalieva","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology, Sechenov University, Trubetskaya St., 8\/2, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Pugacheva","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology, Sechenov University, Trubetskaya St., 8\/2, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3763-7454","authenticated-orcid":false,"given":"Vladimir","family":"Schekin","sequence":"additional","affiliation":[{"name":"Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia"},{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples\u2019 Friendship University of Russia (RUDN University), Miklouho-Maclay St., 6, 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makka","family":"Bimurzaeva","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology, Sechenov University, Trubetskaya St., 8\/2, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1339-5422","authenticated-orcid":false,"given":"Svetlana","family":"Pesegova","sequence":"additional","affiliation":[{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples\u2019 Friendship University of Russia (RUDN University), Miklouho-Maclay St., 6, 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Shegay","sequence":"additional","affiliation":[{"name":"Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Kaprin","sequence":"additional","affiliation":[{"name":"Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia"},{"name":"Department of Urology and Operative Nephrology, Peoples\u2019 Friendship University of Russia (RUDN University), Miklouho-Maclay St., 6, 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Reiser, E., Bazzano, M.V., Solano, M.E., Haybaeck, J., Schatz, C., Mangesius, J., Ganswindt, U., and Toth, B. 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