{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:26:32Z","timestamp":1760145992724,"version":"build-2065373602"},"reference-count":59,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Erasmus Mundus Joint Master Degree (EMJMD) program, funded by the European Union"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study investigates the immobilization of cyanobacterial photosystem I (PSI) from Synechocystis sp. PCC 6803 onto fluorine-doped tin oxide (FTO) conducting glass plates to create photoelectrodes for biohybrid solar cells. The fabrication of these PSI\u2013FTO photoelectrodes is based on two immobilization processes: rapid electrodeposition driven by an external electric field and slower adsorption during solvent evaporation, both influenced by gravitational sedimentation. Deposition and performance of photoelectrodes was investigated by UV\u2013Vis absorption spectroscopy and photocurrent measurements. We investigated the efficiency of PSI immobilization under varying conditions, including solution pH, applied electric field intensity and duration, and electrode polarization, with the goals to control (1) the direction of migration and (2) the orientation of the PSI particles on the substrate surface. Variation in the pH value of the PSI solution alters the surface charge distribution, affecting the net charge and the electric dipole moment of these proteins. Results showed PSI migration to the positively charged electrode at pH 6, 7, and 8, and to the negatively charged electrode at pH 4.4 and 5, suggesting an isoelectric point of PSI between 5 and 6. At acidic pH, the electrophoretic migration was largely hindered by protein aggregation. Notably, photocurrent generation was consistently cathodic and correlated with PSI layer thickness, and no conclusions can be drawn on the orientation of the immobilized proteins. Overall, these findings suggest mediated electron transfer from FTO to PSI by the used electrolyte containing 10 mM sodium ascorbate and 200 \u03bcM dichlorophenolindophenol.<\/jats:p>","DOI":"10.3390\/ijms25189772","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T04:35:24Z","timestamp":1725942924000},"page":"9772","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dependence of Protein Immobilization and Photocurrent Generation in PSI\u2013FTO Electrodes on the Electrodeposition Parameters"],"prefix":"10.3390","volume":"25","author":[{"given":"Theresa","family":"Kehler","sequence":"first","affiliation":[{"name":"Faculty of Physics, Adam Mickiewicz University, ul. Uniwersytetu Pozna\u0144skiego 2, 61-614 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Szewczyk","sequence":"additional","affiliation":[{"name":"Faculty of Physics, Adam Mickiewicz University, ul. Uniwersytetu Pozna\u0144skiego 2, 61-614 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1803-6282","authenticated-orcid":false,"given":"Krzysztof","family":"Gibasiewicz","sequence":"additional","affiliation":[{"name":"Faculty of Physics, Adam Mickiewicz University, ul. Uniwersytetu Pozna\u0144skiego 2, 61-614 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/S0005-2728(96)00112-0","article-title":"Electron transfer and arrangement of the redox cofactors in photosystem I","volume":"1318","author":"Brettel","year":"1997","journal-title":"Biochim. Biophys. Acta Bioenerg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0005-2728(01)00202-X","article-title":"Electron transfer in Photosystem I","volume":"1507","author":"Brettel","year":"2001","journal-title":"Biochim. Biophys. 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